Conformation of bovine-serum albumin for various degrees of iodination using electron spin resonance

1984 ◽  
Vol 4 (3) ◽  
pp. 327-334 ◽  
Author(s):  
C. Chagalj ◽  
T. C. P. DePaoli ◽  
A. A. Hager ◽  
E. Rubin de Celis ◽  
H. A. Farach ◽  
...  
Keyword(s):  
Electron Spin Resonance ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Electron Spin ◽  
Bovine Serum ◽  
Spin Resonance

Nitroxides XLVIII: A study of the interaction between bovine serum albumin and a modified steroid by electron spin resonance

FEBS Letters ◽  
1971 ◽  
Vol 19 (1) ◽  
pp. 55-59 ◽  
Author(s):  
E. Chambaz ◽  
G. Defaye ◽  
A. Hadjian ◽  
P. Martin ◽  
R. Ramasseul ◽  
...  
Keyword(s):  
Electron Spin Resonance ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Electron Spin ◽  
Bovine Serum ◽  
Spin Resonance

scholarly journals Electron Spin Resonance Spectroscopy of Serum Albumin: A Novel New Test for Cancer Diagnosis and Monitoring

2006 ◽  
Vol 52 (11) ◽  
pp. 2129-2134 ◽  
Author(s):  
Steven C Kazmierczak ◽  
Andrey Gurachevsky ◽  
Gert Matthes ◽  
Vladimir Muravsky
Keyword(s):  
Electron Spin Resonance ◽  
Stearic Acid ◽  
Serum Albumin ◽  
Cancer Patients ◽  
Electron Spin ◽  
Spin Probe ◽  
Esr Spectroscopy ◽  
Resonance Spectroscopy ◽  
Esr Spectrum ◽  
Spin Resonance

Abstract Background: Proteins released by tumor cells can bind to serum albumin, leading to structural and functional modifications. We used electron spin resonance (ESR) spectroscopy to measure these changes in serum albumin and evaluate their utility for the diagnosis and monitoring of cancer. Methods: We used an ESR spectrometer and 16-doxyl stearic acid as spin probe to measure conformational changes in albumin in blood samples from a population of healthy donors and volunteers (n = 349), patients with a wide variety of hematologic and nonhematologic malignancy (n = 135), and patients with chronic diseases such as gastrointestinal and pulmonary disease, diabetes, and cirrhosis (n = 91). We added differing amounts of 16-doxyl stearic acid spin probe in ethanol to 50 μL of serum from each patient to create 3 different aliquots that differed in concentration of spin probe and ethanol, then incubated the aliquots for 10 min at 37 °C with continuous shaking. We measured the ESR spectra of each aliquot in triplicate and used proprietary software (MedInnovation GmbH) to evaluate the ESR spectrum for differences between cancer patients and the other groups. Results: The diagnostic sensitivity and specificity of this test were 87.4% and 95.7%, respectively, for differentiating healthy individuals from cancer patients and 87.4%, and 85.7% for differentiating cancer patients from chronic disease patients. Serial evaluation of albumin conformation changes in several patients followed during the course of their disease showed excellent agreement between the magnitude of abnormality in the ESR spectrum of albumin and clinical and pathologic estimates of disease severity. Conclusions: ESR spectroscopy of serum albumin is a sensitive and noninvasive technique that clearly demonstrates diagnostic utility in patients with cancer. This test also enables monitoring of the disease course through use of serial measurements.

Notizen: Interaction of Ascorbic Acid with Disulfides

1983 ◽  
Vol 38 (9-10) ◽  
pp. 859-861 ◽  
Author(s):  
James E. Fleming ◽  
Klaus G. Bensch ◽  
Jörg Schreiber ◽  
Wolfgang Lohmann
Keyword(s):  
Ascorbic Acid ◽  
Electron Spin Resonance ◽  
Bovine Serum Albumin ◽  
Redox Potential ◽  
Electron Spin ◽  
Disulfide Bond ◽  
Gamma Globulin ◽  
Oxidized Glutathione ◽  
Acid System ◽  
Spin Resonance

Electron spin resonance (EPR) spectra demonstrate the formation of a complex between the ascorbate ion and disulfides such as oxidized glutathione, cystine, gamma globulin, bovine serum albumin, 4-4′ dithiopyridine, and dithiobutyric acid. However, except in the case of 4-4′ dithiopyridine. ascorbic acid (AH2) is not capable of reducing the disulfide bond in these compounds. These results can be explained in terms of the redox potential of ascorbic acid and its radical semidehydroascorbic acid (SDA). These compounds can reduce the S-S bond of 4-4′ dithiopyridine, the redox potential of which is E′ = -0. 1 V and, thus, more positive than the ones deter­mined for the ascorbic acid system.

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