Identification of specific binding sites for nerve growth factor on human blood platelets and membranes from bovine brain

1996 ◽  
Vol 121 (3) ◽  
pp. 271-273 ◽  
Author(s):  
N. D. Chekalina ◽  
T. P. Klyushnik ◽  
O. S. Brusov ◽  
E. V. Danilovskaya ◽  
N. L. Deineko
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Human Blood ◽  
Binding Sites ◽  
Specific Binding ◽  
Blood Platelets ◽  
Bovine Brain ◽  
Nerve Growth ◽  
Specific Binding Sites ◽  
Human Blood Platelets

Specific binding sites for 125I-nerve growth factor in peripheral tissues and brain

1974 ◽  
Vol 57 (4) ◽  
pp. 1096-1103 ◽  
Author(s):  
William A. Frazier ◽  
Linda F. Boyd ◽  
Andrzej Szutowicz ◽  
Morris W. Pulliam ◽  
Ralph A. Bradshaw
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Binding Sites ◽  
Specific Binding ◽  
Peripheral Tissues ◽  
Nerve Growth ◽  
Specific Binding Sites

Interaction of Vasopressin with Human Blood Platelets: Dependency on Mg2+

1986 ◽  
Vol 56 (03) ◽  
pp. 260-262 ◽  
Author(s):  
Isabella Roos ◽  
Fabrizia Ferracin ◽  
Alfred Pletscher
Keyword(s):  
Phosphatidic Acid ◽  
Human Blood ◽  
Arginine Vasopressin ◽  
Specific Binding ◽  
Blood Platelets ◽  
Bivalent Cations ◽  
Platelet Membranes ◽  
Maximal Rise ◽  
Human Blood Platelets ◽  
Stimulation Of

SummaryArginine-vasopressin (AVP) in the presence of Mg2+ but not in the absence of bivalent cations led to accumulation of [32P]-phosphatidic acid ([32P]-PA) in human blood platelets. Mg2+ also enhanced the specific binding of [3H]-AVP to intact platelets. The concentrations of the cation which enabled AVP to cause half maximal rise of [32P]-PA and those inducing half maximal [3H]-AVP-binding were of the same order. It is concluded that the stimulation of phosphatidyl inositide breakdown by AVP in presence of Mg2+ is at least partially due to a Mg2+-induced enhancement of specific AVP-binding to the platelet membranes.

The effect of nerve growth factor on the development of sodium channels in PC12 cells

1986 ◽  
Vol 64 (11) ◽  
pp. 1153-1159 ◽  
Author(s):  
Juta K. Reed ◽  
Diane England
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Pc12 Cells ◽  
Specific Binding ◽  
Neuronal Cell ◽  
Scorpion Toxin ◽  
Excitable Cells ◽  
Response Curves ◽  
Nerve Growth ◽  
Differentiated Cells

We have studied the development of the action potential Na+ channels in PC12 cells, an established line that has been useful as a model for neuronal differentiation. In continuous culture PC12 cells, although electrically inexcitable, nevertheless have a low level of Na+ channels as judged by the increase in 22Na+ uptake in the presence of veratridine and scorpion toxin. These two neurotoxins have been shown to promote activation of Na+ channels in a variety of electrically excitable cells. Following treatment with nerve growth factor (NGF), conditions which induce differentiation to an electrically excitably neuronal-cell type, the neurotoxin-activated 22Na+ uptake increases approximately 12-fold, on a per cell basis, reaching a maximum in 12–16 days. The dose–response curves for veratridine and scorpion toxin are unchanged by NGF treatment (K0.5 for veratridine, 18–14 μM; K0.5 for scorpion toxin, 120–96 nM). Na+ channels in both undifferentiated and differentiated cells are tetrodotoxin sensitive and NGF treatment has no effect on the inhibition constant (Ki, 10–12 nM). Na+ channel sites were measured directly by the specific binding of [3H]saxitoxin. In NGF-treated cells, the saxitoxin receptor density reaches 154 fmol/mg protein (Kd, 1.3 nM), a level comparable to other excitable cells. Levels in control cells were too low to measure accurately. These findings show that NGF treatment of PC12 cells leads to a substantial increase in the expression of neurotoxin-sensitive Na+ channels. Furthermore, these channels are pharmacologically similar, if not identical, to those which exist in undifferentiated cells and therefore do not appear to result from the conversion of preexisting channels.

Nerve growth factor binding sites on hepatic parenchymal cells

1992 ◽  
Vol 28 (9-10) ◽  
pp. 621-624 ◽  
Author(s):  
M. B. Bailie ◽  
R. A. Roth ◽  
M. L. Contreras
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Binding Sites ◽  
Nerve Growth ◽  
Factor Binding ◽  
Parenchymal Cells
Sign in / Sign up

Export Citation Format

Share Document