Potassium transport through the erythrocyte plasma membrane in patients with insulin-dependent diabetes mellitus: Effects of insulin therapy

1996 ◽  
Vol 122 (1) ◽  
pp. 748-751
Author(s):  
A. A. Kubatiev ◽  
T. S. Balashova ◽  
M. I. Balabolkin ◽  
E. N. Tomilova
Keyword(s):  
Diabetes Mellitus ◽  
Plasma Membrane ◽  
Insulin Therapy ◽  
Insulin Dependent Diabetes Mellitus ◽  
Potassium Transport ◽  
Insulin Dependent Diabetes ◽  
Dependent Diabetes Mellitus ◽  
Insulin Dependent ◽  
Erythrocyte Plasma Membrane

Variations of 1-deoxyglucose(1,5-anhydroglucitol) content in plasma from patients with insulin-dependent diabetes mellitus.

1983 ◽  
Vol 29 (7) ◽  
pp. 1396-1398 ◽  
Author(s):  
S Yoshioka ◽  
S Saitoh ◽  
C Negishi ◽  
T Fujisawa ◽  
A Fujimori ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Insulin Therapy ◽  
Insulin Dependent Diabetes Mellitus ◽  
Insulin Dependent Diabetes ◽  
Dependent Diabetes Mellitus ◽  
Glass Capillary Column ◽  
Diabetic Patients ◽  
Gas Liquid Chromatography ◽  
Glass Capillary ◽  
Insulin Dependent

Abstract The concentration of 1-deoxyglucose(1,5-anhydroglucitol) in plasma from patients with insulin-dependent diabetes mellitus was measured by gas-liquid chromatography with an all-glass capillary column. Twenty-one plasma samples were obtained from 21 patients before insulin therapy, and 34 more from 13 patients receiving insulin therapy. 1-Deoxyglucose was generally not detectable in plasmas of diabetic patients before they received insulin; it was measurable in the patients who had received insulin, although its concentration was low compared with that of healthy subjects. We therefore suggest that the absence of 1-deoxyglucose in plasma is one of the markers of metabolic states of diabetes, perhaps reflecting a disturbed function of carbohydrate metabolism; its presence in plasma within a normal range may reflect the better control of diabetic patients.

GLUT-4 content in plasma membrane of muscle from patients with non-insulin-dependent diabetes mellitus

1993 ◽  
Vol 265 (6) ◽  
pp. E889-E897 ◽  
Author(s):  
S. Lund ◽  
H. Vestergaard ◽  
P. H. Andersen ◽  
O. Schmitz ◽  
L. B. Gotzsche ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Plasma Membrane ◽  
Insulin Dependent Diabetes Mellitus ◽  
Human Muscle ◽  
Insulin Dependent Diabetes ◽  
Dependent Diabetes Mellitus ◽  
Crude Membrane ◽  
Glut 4 ◽  
Insulin Dependent ◽  
Niddm Patients

The abundance of GLUT-4 protein in both total crude membrane and plasma membrane fractions of vastus lateralis muscle from 13 obese non-insulin-dependent diabetes mellitus (NIDDM) patients and 14 healthy subjects were examined in the fasting state and after supraphysiological hyperinsulinemia. In the basal state the immunoreactive mass of GLUT-4 protein both in the crude membrane preparation and in the plasma membrane fraction was similar in NIDDM patients and control subjects. Moreover, in vivo insulin exposure neither for 30 min nor for 4 h had any impact on the content of GLUT-4 protein in plasma membranes. With the use of the same methodology, antibody, and achieving the same degree of plasma membrane purification and recovery, we found, however, that intraperitoneal administration of insulin to 7-wk-old rats within 30 min increased the content of GLUT-4 protein more than twofold (P < 0.01) in the plasma membrane from red gastrocnemius and soleus muscle. In conclusion, when the subcellular fractionation method was applied to human muscle biopsies taken in the basal state, no difference could be found in the plasma membrane content of immunoreactive GLUT-4 protein between NIDDM patients and normal subjects. With this technique, we were unable to show evidence for a regulatory effect of insulin on the plasma membrane level of GLUT-4 protein in human muscle.

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