Effect of activation of Ca2+, Mg2+-dependent endonuclease on proliferative response of human peripheral blood lymphocytes

1997 ◽  
Vol 123 (5) ◽  
pp. 465-467
Author(s):  
G. V. Shmarina ◽  
E. L. Zyuzina
Keyword(s):  
Peripheral Blood ◽  
Proliferative Response ◽  
Human Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Blood Lymphocytes ◽  
Human Peripheral Blood Lymphocytes ◽  
Dependent Endonuclease

Intracellular free magnesium in human lymphocytes and the response to lectins

1991 ◽  
Vol 80 (6) ◽  
pp. 539-547 ◽  
Author(s):  
L. L. Ng ◽  
J. E. Davies ◽  
M. C. Garrido
Keyword(s):  
Phaseolus Vulgaris ◽  
Peripheral Blood ◽  
Proliferative Response ◽  
Human Peripheral Blood ◽  
Human Lymphocytes ◽  
Peripheral Blood Lymphocytes ◽  
Blood Lymphocytes ◽  
Fluorimetric Method ◽  
Human Peripheral Blood Lymphocytes ◽  
Free Magnesium

1. Intracellular free [Mg2+] was measured in human peripheral blood lymphocytes using a fluorimetric method based on the dye furaptra. It was necessary to correct for the extracellular leakage of the dye by using either 10 mmol/l EDTA or 0.05 mmol/l Mn2+. 2. As the proliferative response of lymphocytes to mitogenic lectins has been linked to a dependence on extracellular Mg2+, the intracellular [Mg2+] was studied in lymphocytes stimulated with various mitogenic and non-mitogenic lectins. 3. Only lymphocytes treated with phytohaemagglutinin-L, a leucoagglutinin from Phaseolus vulgaris that binds to tri- and tetra-antennary complex glycoproteins, showed a marked increase in intracellular [Mg2+]. This effect was partially inhibited by N-acetylgalactosamine. The stimulation by different lectins of the incorporation of [3H]-thymidine into lymphocytes was not correlated to the changes in intracellular [Mg2+]. 4. The proliferative response of lymphocytes to lectins is therefore not wholly dependent on a rise in intracellular [Mg2+].

The proliferative response of human peripheral blood lymphocytes to group a streptococcal vaccine

1987 ◽  
Vol 43 (3) ◽  
pp. 314-324 ◽  
Author(s):  
Andreas Morell ◽  
Ursula Stoffel-Mazenauer ◽  
Giuseppe Vassalli ◽  
Walter F. Riesen ◽  
Dietmar G. Braun
Keyword(s):  
Peripheral Blood ◽  
Proliferative Response ◽  
Human Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Blood Lymphocytes ◽  
Human Peripheral Blood Lymphocytes ◽  
Group A
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