Free radical oxidation and antioxidant activity in placental tissue in preterm labor

1997 ◽  
Vol 124 (6) ◽  
pp. 1182-1184
Author(s):  
V. M. Prokopenko ◽  
A. V. Arutyunyan ◽  
E. V. Frolova ◽  
T. U. Kuz'minykh ◽  
E. K. Ailamazyan
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Preterm Labor ◽  
Free Radical Oxidation ◽  
Placental Tissue ◽  
Radical Oxidation

scholarly journals Specific features of biological oxidation processes in helminthiases of horses

2020 ◽  
Vol 61 (3) ◽  
pp. 126-130
Author(s):  
Larisa V. Pilip ◽  
◽  
Olga V. Byakova ◽  
Maria E. Kazakova ◽  
◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Free Radical ◽  
Free Radical Oxidation ◽  
The Body ◽  
Luminous Intensity ◽  
Radical Oxidation ◽  
Pathological Conditions ◽  
Oxidized Products

The chemical processes occurring in the animal body under pathological conditions are of interest to researchers. The objective of the research was to study the indicators of lipid peroxidation and antioxidant activity in horses infested with helminthiases and treated with anthelmintic paste with an antioxidant. The study has proved the role of parasitic infestation in accelerating the processes of free radical oxidation, which is manifested by a decrease in the light sum of radicals (36.62-39.21), the maximum luminous intensity (6.13-6.98) and antioxidant activity (5.65-6.2) at the start of the research. Chronic invasion increases the concentration of under-oxidized products up to 38.59, and the rate of initiating free radical oxidation – up to 6.95, the values of antioxidant activity being stably low (5.51-5.91). An important role in the regulation of lipid peroxidation in horses during helminthiases, as well as in the process of deworming, is played by the antioxidants contained in anthelmintic preparations. The antioxidant santohin included as a compound in the «Alezan» preparation suppresses enhanced peroxidation and activates the antioxidant protection of the body, which is reflected in a decrease in the light sum of the radicals to as low as 26.73, a maximum luminous intensity to – 3.76, and an increase in antioxidant activity – up to 7.16.

scholarly journals Critical conditions as a logical and appropriate chain of iron methabolism disorders (the summary of experimental studies)

2013 ◽  
Vol 59 (6) ◽  
pp. 700-709 ◽  
Author(s):  
Y.P. Orlov ◽  
V.N. Lukach ◽  
V.T. Dolgih ◽  
E.L. Soboleva ◽  
A.V. Ivanov ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Rheological Properties ◽  
Experimental Studies ◽  
Free Radical Oxidation ◽  
Critical Conditions ◽  
Total Antioxidant Activity ◽  
Radical Oxidation ◽  
Oxidation Processes ◽  
Total Antioxidant

It is revealed that in all models of critical conditions will activate the free-radical oxidation, decreasing the total antioxidant activity, the concentration of transferrin decreases in the serum of the blood, increase of the concentration of ferritin, the rheological properties of the blood are violated and the signs of endothelial dysfunction are identified. Pre-entered deferoxamine in the dose of 80 mg/kg reduced the intensity of free-radical oxidation processes, restoring the antioxidant potential, concentration of the transferrin, and a lower level of ferritin, contributed to the normalization of blood rheological properties and a reduction of the extent endothelium destruction as a result of the reduction Fe2+ concentration in blood serum.

scholarly journals EVALUATION OF ANTIOXIDANT ACTIVITY OF PLANT AGENTS

2020 ◽  
pp. 187-193
Author(s):  
Yekaterina Vladimirovna Ferubko ◽  
Valeriy Nikolayevich Zelenkov ◽  
Anatoliy Andreyevich Lapin ◽  
Tamara Darizhapovna Dargayeva
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Lipid Analysis ◽  
Free Radical Oxidation ◽  
Inhibitory Effect ◽  
Total Antioxidant Activity ◽  
Radical Oxidation ◽  
White Rats ◽  
Total Antioxidant

The aim of the research is to determine the total antioxidant activity of in vitro collection of antihepatotoxic action consisting of roots and rhizomes of Inula helenium L., grass Centaurium erythraea Rafn., flowers Tanacetum vulgare L., fruits Rosa sp., fruits of Crataegus sp. аnd its separate components by the method of colonometric titration and determination of antioxidant activity of dry extract (conditional name "Pentafite") obtained from this collection under conditions of experimental tetrachloride of carbon hepatitis in white rats. A method of chemiluminescent lipid analysis was used to evaluate the free radical oxidation of lipids in liver in test animals. It has been found that collection of antihepatotoxic action and plants included in it have antioxidant activity in vitro, at the same time synergistic effect on manifestation of total antioxidant activity of collection is revealed. Pharmacotherapy of experimental hepatitis in experimental rats by administration of Pentafite at a dose of 300 mg/kg has been found to significantly reduce the chemiluminometric values of lipids, which characterize the intensity of free radical reactions. "Pentafite" has a pronounced inhibitory effect on hyperlipoperoxidation in animal liver in case of toxic damage to it. The established antioxidant activity of collecting antihepatotoxic action and Pentafite extract may be important for their use in the treatment and prevention of hepatobiliary diseases.

scholarly journals Effect of bromfenac on free radical oxidation in model systems

2019 ◽  
Vol 100 (4) ◽  
pp. 636-641 ◽  
Author(s):  
E F Galimova ◽  
Z G Khaibullina ◽  
D A Enikeev ◽  
Yu L Bortsova ◽  
K S Mochalov ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Free Radical ◽  
Reactive Oxygen ◽  
Free Radical Oxidation ◽  
Model Systems ◽  
Oxygen Species ◽  
Radical Oxidation ◽  
Anti Inflammatory

Aim. The study of free radical oxidation processes in an experiment on model systems using the anti-inflammatory drug bromfenac (nakwan) widely used in ophthalmology for the treatment of infectious and inflammatory diseases of the anterior chamber of the eye. Methods. The antioxidant capacity of the drug was evaluated by chemiluminescence registration and analysis of model systems that generate reactive oxygen species and reproduce lipid peroxidation processes using the chemiluminomer CL-003. The following parameters of spontaneous and induced chemiluminescence were determined: light sum and maximum luminescence amplitude, duration of latent period, amplitude of fast and slow flash. Results. When tested in vitro in two different model systems, a high antioxidant activity of the studied drug was established, up to complete suppression of chemiluminescence when 90 μg of bromfenac was added to the incubation medium, which characterizes the inhibition of the generation of reactive oxygen species. A significant increase in total antioxidant activity with bromfenac was also demonstrated, which is reflected by the integral parameter of chemiluminescence — light sum which decreased with the introduction of 10 μg of the drug by 1.2 times, and with 90 μg by 1.5 times. A comparative analysis of the antioxidant properties of various nonsteroidal anti-inflammatory drugs used in ophthalmic practice demonstrated a more pronounced efficacy of bromfenac compared to ketorolac, the use of which was not accompanied by statistically significant changes in chemiluminescence. A very important mechanism of the positive effect of bromfenac is the direct dependence of the action on its quantity in the reaction medium, which opens up prospects for the controlled correction of free radical phenomena and the excessive activation of lipid peroxidation in the imbalance of the pro- and antioxidant processes in biological systems. Conclusion. It is suggested that the protective effects of the drug in various infectious-inflammatory lesions of the eye can be determined, along with previously known properties, its antioxidant activity, restriction of increased production of reactive oxygen species and oxidative stress phenomena.

scholarly journals UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAUN BUAS-BUAS (Premna serratifolia L.) ASAL KABUPATEN MELAWI PROVINSI KALIMANTAN BARAT DENGAN METODE DPPH

2020 ◽  
Vol 3 (2) ◽  
pp. 405-412
Author(s):  
Weni Puspita ◽  
◽  
Dina Yuspita Sari ◽  
Ika Ristia Rahman ◽  
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Free Radical ◽  
Cell Damage ◽  
Antioxidant Properties ◽  
Free Radical Oxidation ◽  
The Body ◽  
Radical Oxidation ◽  
Ic50 Values ◽  
Ethanol Extracts

Buas (Premna serratifolia L.) contains secondary metabolites of flavonoids, saponins, tannins and triterpenoids/steroids, where flavonoids are known to have antioxidant properties and counteract free radicals. Antioxidants are compounds that can fight toxic hazards and reduce cell damage to the body caused by free radical oxidation. The purpose of this study was to determine the antioxidant activity of ethanol extracts of buas-buas leaves was carried out by DPPH (2,2-diphenyl-1-picrylhydrazyl), using UV-Vis spectrophotometry at wavelength 518.40 nm with various concentrations of 10 ppm, 20 ppm, 30 ppm, 40 ppm and 50 ppm. The results showed that Buas Buas leaves (Premna serratifolia L.) had an antioxidant activity with IC50 values ??of 20.66 ug / mL.

scholarly journals Pathogenic Significance of Membrane and Metabolic Disorders in Paediatric Bronchitis

Doctor Ru ◽  
2021 ◽  
Vol 20 (10) ◽  
pp. 18-24
Author(s):  
E.A. Samorodnova ◽  
◽  
R.A. Faizullina ◽  
O.I. Pikuza ◽  
A.M. Zakirova ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Free Radical Oxidation ◽  
Mean Value ◽  
Healthy Children ◽  
Study Objective ◽  
Normal Value ◽  
Radical Oxidation ◽  
Clinical Recovery ◽  
Study Results

Study Objective: To study the features of intracellular and extracellular free-radical oxidation (FRO) processes with reference to the cellular membrane condition in various clinical forms of paediatric bronchitis. Study Design: prospective cohort study. Materials and Methods. The study included 237 children aged 2 to 15.5 years old with bronchitis. The patients were divided into three groups depending on the clinical form of bronchitis: acute bronchitis (AB) — 91 children (mean age: 9.8 years old); acute obstructive bronchitis (AOB) — 32 children (mean age: 7.5 years old); and recurrent bronchitis (RB) — 14 children (mean age: 10.2 years old). The control group included 43 conditionally healthy children (mean age: 9.5 years old). We conducted clinical examination, as well as standard instrumental and laboratory tests and interviewed all children. We studied FRO activity (using spontaneous and zymozan-induced luminol-depend chemicoluminescence, ICL), cumulative serum antioxidant activity (AOA), and ceruleoplasmin (CP). Red-cell membranes permeability by sodium was measured using determination of the maximum rate of Na+-Li+-exchange transport (ET) in RBC. Study Results. All patients demonstrated increased rate of Na+-Li+-ET, with the mean value in AB being 232 ± 7.0 (р < 0.05), in OAB — 269 ± 10.0 (р < 0.05), in RB — 328 ± 11.0 μmol Li (р < 0.01). In AB, ICL level was relatively constant vs. normal value irrespective of the disease phase (137.61 ± 22.3 counts/min/1,000 PMNL). In early OAB, ICL was significantly more active (up to 208.98 ± 7.6 counts/min/1,000 PMNL) which normalised later at clinical recovery. In patients with RB, peak ICL was as low as 107.06 ± 26.42 counts/min/1,000 PMNL; at clinical recovery, the value was even lower — 66.41 ± 8.9 counts/min/1,000 PMNL (р < 0.001 vs. healthy children). In acute phase of all forms of the disease, we recorded a significant raise in malondialdehyde; however, its peak concentration was seen in patients with RB; the value was significantly different not only from controls, but also from patients with AB and OAB; and at clinical stabilisation, the value reached 3.72 ± 0.08 μmol (р < 0.05). Patients with AB at recovery had the cumulative AOA of 39.9 ± 0.59% (р < 0.05); however, the control value was not achieved (42.39 ± 0.49%). CP levels were raised as well and reached 64.98 ± 0.43 mg%, but did not differ significantly from the initial value. In OAB, the cumulative OAO did not reach its peak value. Its mean value remained the same. In patients with RB, the cumulative AOA did not reach the normal value, but also reduced a lot (р < 0.05), together with reduced serum CP concentration from 60.20 ± 0.52 mg% to 57.83 ± 0.41 mg% (р < 0.05). Conclusion. Persistence and intensity of membrane and metabolic shifts in children with bronchitis are of great significance in the pathogenesis of a recurrent inflammation in bronchi and can be used as prediction criteria of the disease, allowing to set up a management plan. Keywords: children, bronchitis, free-radical oxidation of lipids, ion exchange system, antioxidant activity, ceruleoplasmin.

scholarly journals Association of blood and liver free radical oxidation indicators in rats with different resistance to hypoxia after survived anoxia

2015 ◽  
Vol 96 (5) ◽  
pp. 798-802
Author(s):  
G A Bayburina ◽  
E A Nurgaleeva ◽  
S A Bashkatov ◽  
D Z Shibkova
Keyword(s):  
Antioxidant Activity ◽  
Factor Analysis ◽  
Free Radical ◽  
Free Radical Oxidation ◽  
Thiobarbituric Acid ◽  
Systemic Circulation ◽  
Antioxidant Systems ◽  
Radical Oxidation ◽  
Resistance To Hypoxia ◽  
White Rats

Aim. To study the association of rat liver and blood free radical oxidation indicators with different resistance to hypoxia on the long run after ischemic damage caused by systemic circulation arrest. Methods. Circulatory arrest lasting for 5 minutes was modeled at ether anesthesia on male white rats which were further allocated to 3 groups according to resistance to hypoxia. The observation period was 35 days. The levels of reduced glutathione, catalase, and products reacting with thiobarbituric acid were determined in liver homogenates, levels of erythrocyte catalase, total antioxidant activity, glutathione peroxidase, glutathione transferase, and products reacting with thiobarbituric acid - in blood. Statistical analysis was performed by parametric statistics, regression and factor analysis. Results. Rats with different resistance to hypoxia initially differed significantly on the number of safety systems functioning indicators levels in liver tissue and blood cells. Total factor analysis of the entire body of empirical data revealed two factors, conventionally called «resistance to hypoxia» and «antioxidant activity of blood». Daily analysis of the figures recorded at 1, 3, 5, 7, 14, 21 and 35 day of the experiment revealed similarities in common and daily factor solutions, demonstrating the objectivity of the revealed patterns, which emphasizes the significant role of free radical oxidation in anoxic conditions pathogenesis. Conclusion. The balance pro- and antioxidant systems activity in the liver and blood is an essential component of the body’s resistance to hypoxia affecting the survival of the animals after systemic circulation arrest. Their level predicts resistance to hypoxia at the recovery period.

State of the enzyme link of antioxidant protection in boys with hypoandrogenism

2020 ◽  
Vol 40 (4) ◽  
pp. 32-36
Author(s):  
L. K. Parkhomenko ◽  
◽  
L. A. Strashok ◽  
S. I. Turchina ◽  
G. V. Kosovtsova ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Free Radical ◽  
Glutathione Peroxidase ◽  
Free Radical Oxidation ◽  
Conjugated Dienes ◽  
Delayed Puberty ◽  
Control Group ◽  
Antioxidant Protection ◽  
Radical Oxidation

Recently, interest in the problem of free radical oxidation in biological membranes, which is directly related to both the normal functioning of cells and the occurrence, course and outcome of many pathological conditions, has increased again in clinical medicine. The aim was to determine the role and impact of antioxidant defense in boys with hypoandrogenism. The study involved 75 adolescents with hypoandrogenism aged 13–18 years, who underwent a complex of clinical and laboratory examinations. All patients were conducted complex of anthropometric research and determination of the degree of delayed puberty, laboratory and instrumental examination. Free radical oxidation was determined by the levels of malondialdehyde, conjugated dienes, carbonated proteins, superoxide dismutase and catalase in the serum, and restored glutathione and glutathione peroxidase in whole blood. Based on their determination, the coefficient of oxidative stress was calculated. Statistical processing of results was performed using parametric and nonparametric methods. The study of indicators of the free radical oxidation process found that adolescents with hypoandrogenism have multidirectional changes in the oxidation of proteins and lipids, namely: the level of conjugated dienes increases, the concentration of malondialdehyde remains at the level of the control group, and the level of carbonated proteins tends to decrease. As for the activity of antioxidant protection enzymes, a significant decrease in the level of glutathione peroxidase was detected, while the level of superoxide dismutase and catalase remained at the level of normative indicators. Oxidative stress accompanies and is one of the pathogenetic links in the formation or maintenance of the state of hypoandrogenism in boys. This requires the use of antioxidants, the complex of which must be selected individually.

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