The use of a nonopiate leu-enkephalin analog and of hydra peptide morphogen for the correction of proliferation disturbances in tracheal epithelium and of LPO processes in lungs of newborn rats exposed to prenatal hypoxia

1994 ◽  
Vol 117 (5) ◽  
pp. 536-538
Author(s):  
O. A. Lebed'ko ◽  
S. S. Timoshin
Keyword(s):  
Prenatal Hypoxia ◽  
Tracheal Epithelium ◽  
Newborn Rats ◽  
Hydra Peptide Morphogen

Effect of the hydra peptide morphogen on posthypoxic disorders in rats exposed to prenatal hypoxia

1997 ◽  
Vol 123 (3) ◽  
pp. 232-234 ◽  
Author(s):  
O. A. Lebed'ko ◽  
T. V. Yatsenko ◽  
S. S. Timoshin ◽  
A. Yu. Rubina
Keyword(s):  
Prenatal Hypoxia ◽  
Hydra Peptide Morphogen

Application of stereological analysis of cell volume to isolated myocytes in culture with and without adrenergic innervation

1990 ◽  
Vol 48 (3) ◽  
pp. 416-417
Author(s):  
Jane K. Rosenthal ◽  
Dianne L. Atkins ◽  
William J. Marvin ◽  
Penny A. Krumm
Keyword(s):  
Cardiac Myocytes ◽  
Structural Changes ◽  
Three Dimensional ◽  
Adrenergic Innervation ◽  
Culture Cell ◽  
Serial Sections ◽  
Newborn Rats ◽  
Primary Culture Cell ◽  
Biological Studies ◽  
Cell Volumes

To comprehend structural changes in cardiac myocytes accompanying adrenergic innervation, it is essential that a three dimensional analysis be performed. To date, biological studies which utilize stereological methods have been limited to cells in tissue and in organs. Our laboratory has utilized current stereological techniques for measuring absolute volumes of individual myocytes in primary culture. Cell volumes are calculated for two distinct groups of cells at 96 hours in culture: isolated myocytes and myocytes innervated with adrenergic neurons (Figure 1).Cardiac myocytes are cultured from the ventricular apices of newborn rats. Cells are plated directly onto tissue culture dishes with or without preplated explants from the paravertebral thoracolumbar sympathetic chain. On day four cultures are photographed and marked for one-to-one cell location. Following conventional fixation and embeddment in eponate-12, the cells are relocated and mounted for microtomy. The cells are completely sectioned at 120nm in their parallel orientation to the surface of the dish (Figure 2). Serial sections are collected on formvar coated slotted grids and are recorded in sequence.

Neurodegeneration in newborn rats following general anesthesia

2006 ◽  
Vol 37 (06) ◽  
Author(s):  
P Bittigau ◽  
S Bercker ◽  
T Kerner ◽  
B Bert ◽  
H Fink ◽  
...  
Keyword(s):  
General Anesthesia ◽  
Newborn Rats
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