Development of haploid cell lines from immature barley,Hordeum vulgare, embryos

1984 ◽  
Vol 3 (3) ◽  
pp. 95-97 ◽  
Author(s):  
G. Séguin-Swartz ◽  
L. Kott ◽  
K. J. Kasha
Keyword(s):  
Hordeum Vulgare ◽  
Cell Lines ◽  
Haploid Cell

Front Cover: Proteogenomic Analysis to Identify Missing Proteins from Haploid Cell Lines

PROTEOMICS ◽  
2018 ◽  
Vol 18 (8) ◽  
pp. 1870061
Author(s):  
Seung-Eun Lee ◽  
JongKeon Song ◽  
Korbinian Bösl ◽  
André C. Müller ◽  
Dijana Vitko ◽  
...  
Keyword(s):  
Cell Lines ◽  
Haploid Cell ◽  
Front Cover

scholarly journals A p53-dependent response limits the viability of mammalian haploid cells

2017 ◽  
Vol 114 (35) ◽  
pp. 9367-9372 ◽  
Author(s):  
Teresa Olbrich ◽  
Cristina Mayor-Ruiz ◽  
Maria Vega-Sendino ◽  
Carmen Gomez ◽  
Sagrario Ortega ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Cell Lines ◽  
Chromosome Segregation ◽  
Mammalian Cells ◽  
Embryonic Stem ◽  
Myelogenous Leukemia ◽  
Unstable State ◽  
Haploid Cell ◽  
Diploid Cells

The recent development of haploid cell lines has facilitated forward genetic screenings in mammalian cells. These lines include near-haploid human cell lines isolated from a patient with chronic myelogenous leukemia (KBM7 and HAP1), as well as haploid embryonic stem cells derived from several organisms. In all cases, haploidy was shown to be an unstable state, so that cultures of mammalian haploid cells rapidly become enriched in diploids. Here we show that the observed diploidization is due to a proliferative disadvantage of haploid cells compared with diploid cells. Accordingly, single-cell–sorted haploid mammalian cells maintain the haploid state for prolonged periods, owing to the absence of competing diploids. Although the duration of interphase is similar in haploid and diploid cells, haploid cells spend longer in mitosis, indicative of problems in chromosome segregation. In agreement with this, a substantial proportion of the haploids die at or shortly after the last mitosis through activation of a p53-dependent cytotoxic response. Finally, we show that p53 deletion stabilizes haploidy in human HAP1 cells and haploid mouse embryonic stem cells. We propose that, similar to aneuploidy or tetraploidy, haploidy triggers a p53-dependent response that limits the fitness of mammalian cells.

scholarly journals Proteogenomic Analysis to Identify Missing Proteins from Haploid Cell Lines

PROTEOMICS ◽  
2018 ◽  
Vol 18 (8) ◽  
pp. 1700386 ◽  
Author(s):  
Seung-Eun Lee ◽  
JongKeon Song ◽  
Korbinian Bösl ◽  
André C. Müller ◽  
Dijana Vitko ◽  
...  
Keyword(s):  
Cell Lines ◽  
Haploid Cell

Selection of haploid cell lines from megagametophyte cultures of maritime pine as a DNA source for massive sequencing of the species

2014 ◽  
Vol 118 (1) ◽  
pp. 147-155 ◽  
Author(s):  
I. Arrillaga ◽  
M. A. Guevara ◽  
J. Muñoz-Bertomeu ◽  
D. Lázaro-Gimeno ◽  
E. Sáez-Laguna ◽  
...  
Keyword(s):  
Cell Lines ◽  
Maritime Pine ◽  
Haploid Cell ◽  
Massive Sequencing ◽  
Selection Of

Ultrastructure of Plant Leaf Tissue Infected with Mite-Borne Viral-Like Pathogens

1970 ◽  
Vol 28 ◽  
pp. 178-179 ◽  
Author(s):  
O. E. Bradfute ◽  
R. E. Whitmoyer ◽  
L. R. Nault
Keyword(s):  
Triticum Aestivum ◽  
Zea Mays ◽  
Electron Microscope ◽  
Hordeum Vulgare ◽  
Microscope Study ◽  
Electron Microscope Study ◽  
Leaf Tissue ◽  
Leaf Ultrastructure ◽  
Double Membrane ◽  
Aceria Tulipae

A pathogen transmitted by the eriophyid mite, Aceria tulipae, infects a number of Gramineae producing symptoms similar to wheat spot mosaic virus (1). An electron microscope study of leaf ultrastructure from systemically infected Zea mays, Hordeum vulgare, and Triticum aestivum showed the presence of ovoid, double membrane bodies (0.1 - 0.2 microns) in the cytoplasm of parenchyma, phloem and epidermis cells (Fig. 1 ).

(EB) Virus: Latency and Expression in Transplanted and Cultured Human Cells

1971 ◽  
Vol 29 ◽  
pp. 368-369
Author(s):  
B. G. Uzman ◽  
M. M. Kasac ◽  
H. Saito ◽  
A. Krishan
Keyword(s):  
Cell Lines ◽  
Primary Cultures ◽  
Virus Particles ◽  
Barr Virus ◽  
Virus Latency ◽  
Virus Surveillance ◽  
Cultured Human Cells ◽  
Epstein Barr ◽  
Serial Transplantation ◽  
Tubular Arrays

In conjunction with the cultivation and transplantation of cells from human tumors by the Programs of Microbiology and Immunogenetics, virus surveillance by electron microscopy has been routinely employed. Of particular interest in this regard have been 3 cell lines cultured from lymph nodes or spleen of 2 patients with Hodgkin's disease and 1 patient with Letterer-Siwe's disease. Each of these cell lines when transplanted in Syrian hamster neonates conditioned with anti-lymphocyte serum grew as serially transplantable tumors; from such transplants of the 3 cell lines cell cultures were retrieved.Herpes type virus particles (Figs. 1, 2, 3) were found in the primary cultures of all three lines, in frozen thawed aliquots of same, and in cultures retrieved from their tumors growing by serial transplantation in hamsters. No virus was detected in sections of 25 of the serially transplanted tumors. However, in 10 such tumors there were repeated instances of tubular arrays in the cisternae of the endoplasmic reticulum (Fig. 4). On serologic examination the herpes virus was shown to be the Epstein-Barr virus.

Comparison of Choriocarcinoma and Normal Placenta

1971 ◽  
Vol 29 ◽  
pp. 324-325
Author(s):  
John C. Garancis ◽  
Roland A. Pattillo ◽  
Robert O. Hussa ◽  
Jon V. Straumfjord
Keyword(s):  
Cell Lines ◽  
Small Cells ◽  
Tissue Cultures ◽  
Glycogen Depletion ◽  
Cell Surfaces ◽  
Intercellular Spaces ◽  
Concomitant Increase ◽  
Gestational Choriocarcinoma ◽  
Cytoplasmic Glycogen ◽  
Normal Placenta

Two different cell lines (Be-Wo and Jar) of human gestational choriocarcinoma have been maintained in continuous tissue culture for a period of four and two years respectively without losing the ability to elaborate human chorionic gonadotropin (HCG). Tissue cultures, as revealed by electron microscopy, consisted of small cells with single nuclei. In some instances cell surfaces were provided with microvilli but more often the intercellular spaces were narrow and bridged by desmosomes. However, syncytium was not formed. Endoplasmic reticulum (ER) was poorly developed in both cell lines, except in some Be-Wo cells it was prominent. Golgi complex, lysosomes and numerous free ribosomes, as well as excessive cytoplasmic glycogen, were present in all cells (Fig. 1). Glycogen depletion and concomitant increase of ER were observed in many cells following a single dose of 10 ugm/ml of adrenalin added to medium (Fig. 2).

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