Development and preliminary application of a simple assay of S-mephenytoin 4-hydroxylase activity in human liver microsomes

1993 ◽  
Vol 44 (6) ◽  
pp. 559-562 ◽  
Author(s):  
K. Chiba ◽  
K. Manabe ◽  
K. Kobayashi ◽  
Y. Takayama ◽  
M. Tani ◽  
...  
Keyword(s):  
Human Liver ◽  
Liver Microsomes ◽  
Human Liver Microsomes ◽  
Hydroxylase Activity ◽  
Preliminary Application

Inhibition of Coumarin 7-Hydroxylase Activity in Human Liver Microsomes

1997 ◽  
Vol 341 (1) ◽  
pp. 47-61 ◽  
Author(s):  
Alison J. Draper ◽  
Ajay Madan ◽  
Andrew Parkinson
Keyword(s):  
Human Liver ◽  
Liver Microsomes ◽  
Human Liver Microsomes ◽  
Hydroxylase Activity ◽  
Coumarin 7

Lauric acid hydroxylase activity and cytochrome P450 IV family proteins in human liver microsomes

1991 ◽  
Vol 42 (9) ◽  
pp. 1841-1844 ◽  
Author(s):  
H.A.A.M. Dlrven ◽  
J.G.P. Peters ◽  
G.Gordon Gibson ◽  
W.H.M. Peters ◽  
F.J. Jongeneelen
Keyword(s):  
Cytochrome P450 ◽  
Lauric Acid ◽  
Human Liver ◽  
Liver Microsomes ◽  
Human Liver Microsomes ◽  
Hydroxylase Activity

A radiometric assay for debrisoquine 4-hydroxylase in human liver microsomes

1984 ◽  
Vol 62 (1) ◽  
pp. 84-88 ◽  
Author(s):  
M. Nakano ◽  
T. Inaba
Keyword(s):  
Human Liver ◽  
High Performance ◽  
Liquid Scintillation ◽  
Liver Microsomes ◽  
Microsomal Protein ◽  
Scintillation Counting ◽  
Human Liver Microsomes ◽  
Hydroxylase Activity ◽  
Radiometric Assay ◽  
Layer Chromatography

A radiometric method to assay debrisoquine 4-hydroxylase activity in human liver microsomes was established. Following incubation with 14C-labelled debrisoquine, unreacted debrisoquine was extracted with chloroform; 4-hydroxydcbrisoquine was derivatized with hexafluoroacetylacetone, extracted, and subjected to high performance thin-layer chromatography (HP-TLC) followed by liquid scintillation counting. The Km values for debrisoquine 4-hydroxylase were 70 and 120 μM and the Vmax values were 8 and 24 pmol per milligram microsomal protein per minute. By application of this assay, it was possible to show that 4-hydroxydebrisoquine formation was competitively inhibited by sparteine. Antipyrine up to a concentration of 4 mM had no effect.

CYP2C8 polymorphisms in Caucasians and their relationship with paclitaxel 6α-hydroxylase activity in human liver microsomes

2002 ◽  
Vol 64 (11) ◽  
pp. 1579-1589 ◽  
Author(s):  
Namrata Bahadur ◽  
Julian B.S Leathart ◽  
Elaine Mutch ◽  
Dorothy Steimel-Crespi ◽  
Stuart A Dunn ◽  
...  
Keyword(s):  
Human Liver ◽  
Liver Microsomes ◽  
Human Liver Microsomes ◽  
Hydroxylase Activity

Differential inhibition of coumarin 7-hydroxylase activity in mouse and human liver microsomes

1993 ◽  
Vol 45 (5) ◽  
pp. 1035-1042 ◽  
Author(s):  
Jukka Mäenpää ◽  
Holger Sigusch ◽  
Hannu Raunio ◽  
Tuula Syngelmä ◽  
Pia Vuorela ◽  
...  
Keyword(s):  
Human Liver ◽  
Liver Microsomes ◽  
Human Liver Microsomes ◽  
Differential Inhibition ◽  
Hydroxylase Activity ◽  
Coumarin 7
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