Demonstration of dopa oxidase activity by purified mammalian superoxide dismutase using polyacrylamide gel electrophoretic separation

AGE ◽  
1978 ◽  
Vol 1 (2) ◽  
pp. 56-59
Author(s):  
Thomas E. Brennan ◽  
Harvey C. Shapiro ◽  
Lee M. Edelstein ◽  
L. Michael Snyder
Keyword(s):  
Superoxide Dismutase ◽  
Oxidase Activity ◽  
Polyacrylamide Gel ◽  
Electrophoretic Separation ◽  
Dopa Oxidase

scholarly journals Attenuation of renal excretory responses to ANG II during inhibition of superoxide dismutase in anesthetized rats

2010 ◽  
Vol 298 (2) ◽  
pp. F401-F407 ◽  
Author(s):  
Md. Abdul Hye Khan ◽  
Mohammed Toriqul Islam ◽  
Alexander Castillo ◽  
Dewan Syed Abdul Majid
Keyword(s):  
Superoxide Dismutase ◽  
Nadph Oxidase ◽  
Oxidase Activity ◽  
Ang Ii ◽  
Sod Activity ◽  
Doppler Flowmetry ◽  
Nadph Oxidase Activity ◽  
Blood Flows ◽  
Renal Responses

To examine the functional interaction between superoxide dismutase (SOD) and NADPH oxidase activity, we assessed renal responses to acute intra-arterial infusion of ANG II (0.5 ng·kg−1·min−1) before and during administration of a SOD inhibitor, diethyldithiocarbamate (DETC, 0.5 mg·kg−1·min−1), in enalaprilat-pretreated (33 μg·kg−1·min−1) rats ( n = 11). Total (RBF) and regional (cortical, CBF; medullary; MBF) renal blood flows were determined by Transonic and laser-Doppler flowmetry, respectively. Renal cortical and medullary tissue NADPH oxidase activity in vitro was determined using the lucigenin-chemiluminescence method. DETC treatment alone resulted in decreases in RBF, CBF, MBF, glomerular filtration rate (GFR), urine flow (V), and sodium excretion (UNaV) as reported previously. Before DETC, ANG II infusion decreased RBF (−18 ± 3%), CBF (−16 ± 3%), MBF [−5 ± 6%; P = not significant (NS)], GFR (−31 ± 4%), V (−34 ± 2%), and UNaV (−53 ± 3%). During DETC infusion, ANG II also caused similar reductions in RBF (−20 ± 4%), CBF (−19 ± 3%), MBF (−2 ± 2; P = NS), and in GFR (−22 ± 7%), whereas renal excretory responses (V; −12 ± 2%; UNaV; −24 ± 4%) were significantly attenuated compared with those before DETC. In in vitro experiments, ANG II (100 μM) enhanced NADPH oxidase activity both in cortical [13,194 ± 1,651 vs. 20,914 ± 2,769 relative light units (RLU)/mg protein] and in medullary (21,296 ± 2,244 vs. 30,597 ± 4,250 RLU/mg protein) tissue. Application of DETC (1 mM) reduced the basal levels and prevented ANG II-induced increases in NADPH oxidase activity in both tissues. These results demonstrate that renal excretory responses to acute ANG II administration are attenuated during SOD inhibition, which seems related to a downregulation of NADPH oxidase in the deficient condition of SOD activity.

Oxyresveratrol as the Potent Inhibitor on Dopa Oxidase Activity of Mushroom Tyrosinase

1998 ◽  
Vol 243 (3) ◽  
pp. 801-803 ◽  
Author(s):  
Nam-Ho Shin ◽  
Shi Yong Ryu ◽  
Eun Ju Choi ◽  
Seh-Hoon Kang ◽  
IL-Moo Chang ◽  
...  
Keyword(s):  
Oxidase Activity ◽  
Potent Inhibitor ◽  
Mushroom Tyrosinase ◽  
Dopa Oxidase

Potent Inhibitory Effects of N-Aryl S-Alkylthiocarbamate Derivatives on the Dopa Oxidase Activity of Mushroom Tyrosinase

2005 ◽  
Vol 53 (7) ◽  
pp. 747-749 ◽  
Author(s):  
Kun Ho Lee ◽  
Mamoru Koketsu ◽  
Sang Yoon Choi ◽  
Kang Jin Lee ◽  
Pyeongjae Lee ◽  
...  
Keyword(s):  
Oxidase Activity ◽  
Mushroom Tyrosinase ◽  
Inhibitory Effects ◽  
Dopa Oxidase

scholarly journals The Development of Dopa Oxidase Activity in the Skin and in the Hair Cycle

1961 ◽  
Vol 1961 (2) ◽  
pp. 190-194
Author(s):  
Ken HASHIMOTO ◽  
Kazuo OGAWA ◽  
Walter F. LEVER
Keyword(s):  
Oxidase Activity ◽  
Hair Cycle ◽  
Dopa Oxidase

scholarly journals DISCONTINUOUS ELECTROPHORESIS OF PEPSIN AND PEPSINOGEN IN THIN SHEETS OF POLYACRYLAMIDE GEL

1970 ◽  
Vol 18 (12) ◽  
pp. 853-861 ◽  
Author(s):  
LEW CUNNINGHAM ◽  
ELLEN M. RASCH ◽  
ANN L. LEWIS ◽  
RICHARD HEITSCH
Keyword(s):  
Crystal Violet ◽  
Polyacrylamide Gel ◽  
Thin Sheets ◽  
Electrophoretic Separation ◽  
Standard Glass ◽  
Glass Support

Electrophoretic separation of discrete protein bands from solutions of crystalline pepsin or pepsinogen was accomplished in a system incorporating pH discontinuity and using standard, glass microslides to support thin (0.25-mm) sheets of 15% polyacrylamide gel. Adherence of gel films to a glass support during fixation and staining with crystal violet not only prevented distortion of gels as a result of swelling, but also provided preparations that could be readily scanned with a microspectrophotometer. A number of discrete peptide bands were identified from different samples of purified pepsin. Among the several heterogeneous components found in commercially available samples of crystalline pepsinogen, there was a characteristic impurity, the mobility of which was directly comparable to that of pepsin run under the same conditions of electrophoresis. Specific details of methodology are presented.

Relationship between 4-hydroxyanisole toxicity and dopa oxidase activity for three melanoma cell lines

1997 ◽  
Vol 7 (5) ◽  
pp. 373-381 ◽  
Author(s):  
J Rodriguez-Vicente ◽  
V Vicente-Ortega ◽  
M Canteras-Jordana ◽  
F Calderon-Rubiales
Keyword(s):  
Cell Lines ◽  
Melanoma Cell ◽  
Oxidase Activity ◽  
Dopa Oxidase ◽  
Melanoma Cell Lines
Sign in / Sign up

Export Citation Format

Share Document