Estimation of pristane levels in murine ascites fluid and in purified monoclonal antibody preparations

1992 ◽  
Vol 6 (3) ◽  
pp. 249-254
Author(s):  
T. R. Sykes ◽  
T. K. Woo
Keyword(s):  
Monoclonal Antibody ◽  
Ascites Fluid ◽  
Antibody Preparations

Analysis of glycoforms present in two mouse IgG2a monoclonal antibody preparations

1992 ◽  
Vol 155 (2) ◽  
pp. 241-248 ◽  
Author(s):  
JoséM. Coco-Martin ◽  
Freek Brunink ◽  
Tiny A.M. van der Velden-de Groot ◽  
E. Coen Beuvery
Keyword(s):  
Monoclonal Antibody ◽  
Antibody Preparations ◽  
Mouse Igg2a

scholarly journals QSYP Peptide Sequence Is Selected from Phage Display Libraries by Bovine IgG Contaminants in Monoclonal Antibody Preparations

BioTechniques ◽  
2003 ◽  
Vol 34 (1) ◽  
pp. 132-141 ◽  
Author(s):  
J.M. Jacobs ◽  
B.W. Bailey ◽  
J.B. Burritt ◽  
S.G. Morrison ◽  
R.P. Morrison ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Phage Display ◽  
Peptide Sequence ◽  
Phage Display Libraries ◽  
Antibody Preparations

Efficient conjugation of DTPA to an IgM monoclonal antibody in ascites fluid

1988 ◽  
Vol 15 (3) ◽  
pp. 285-292
Author(s):  
Berma M. Kinsey ◽  
Roger M. Macklis ◽  
James M. Ferrara ◽  
Warren W. Layne ◽  
Steven J. Burakoff ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Ascites Fluid

scholarly journals Platelet glycoproteins IIb and IIIa associated with blood monocytes are derived from platelets

Blood ◽  
1986 ◽  
Vol 67 (1) ◽  
pp. 207-213
Author(s):  
RB Levene ◽  
EM Rabellino
Keyword(s):  
Monoclonal Antibody ◽  
Sodium Dodecyl Sulfate ◽  
Protein A ◽  
Sodium Dodecyl ◽  
Blood Monocyte ◽  
Platelet Glycoprotein ◽  
Receptor Complex ◽  
Blood Monocytes ◽  
Silver Stain ◽  
Antibody Preparations

Platelet glycoprotein IIb/IIIa (GP IIb/IIIa), the receptor complex for fibrinogen, has been regarded as a megakaryocyte/platelet lineage- restricted antigen. Recently, however, it has been reported that GP IIb/IIIa is expressed in blood monocytes. Studies were performed to establish the origin and immunological characteristics of monocyte- associated glycoproteins IIb and IIIa (GPs IIb and IIIa). Preparations of blood monocytes containing varying platelet-monocyte ratios were metabolically labeled with [35S]methionine with the expectation that any newly synthesized GPs IIb and IIIa would be monocyte-derived, since platelets have only rudimentary protein synthetic apparatuses. Analyses of sodium dodecyl sulfate (SDS) gels of homogenates of cell preparations containing from 200 to 5:1 platelet-monocyte ratios revealed that unlabeled GPs IIb and IIIa were readily immunoisolated using protein A-Sepharose immunobeads. However, fluorographic analyses of the same cell preparations pulse-labeled with [35S]methionine failed to demonstrate synthesis of GP IIb or IIIa. Additionally, no GP IIb or IIIa was detected when immunoisolation was carried out in pure preparations of monocytes containing less than 1:100 platelet-monocyte ratios and SDS acrylamide gels were stained by the sensitive silver stain method. Furthermore, heterologous polyspecific antisera and two monoclonal antibody preparations against GPs IIb and IIIa, which bound to platelets, failed to bind to monocyte membranes. Thus, evidence was presented that indicated that monocytes do not synthesize platelet GPs IIb and IIIa and that detection of these molecules in blood monocyte preparations reflects platelet contamination.

Monoclonal Antibody-Based ELISA for Detection of Clostridium perfringens Alpha-Toxin

1996 ◽  
Vol 59 (6) ◽  
pp. 621-625 ◽  
Author(s):  
ZONGLIN L. LIU ◽  
HANS P. BLASCHEK
Keyword(s):  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Phospholipase C ◽  
Clostridium Perfringens ◽  
Ascites Fluid ◽  
Quadratic Model ◽  
Alpha Toxin ◽  
Antigen Specificity ◽  
Ethanesulfonic Acid ◽  
Culture Supernatants

A monoclonal antibody-based ELISA was developed to detect alpha-toxin present in Clostridium perfringens bacterial cell lysates and cell-free culture supernatants, Monoclonal antibodies against C. perfringens alpha-toxin were produced in hybridoma tissue culture supernatants and in BALB/c mice ascites fluid, The monoclonal antibodies obtained from hybridoma culture supernatant and ascites fluid showed identical antigen specificity, but the latter showed a higher titer, with a 50% endpoint at 1/4,000. The monoclonal antibodies were specific for phospholipase C produced by C. perfringens, but not by Bacillus cereus. The lower limit of phospholipase C detection was 16 ng/ml. The dose-dependent relationship between absorption at 490 nm and concentration of phospholipase C diluted in HEPES (N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid) or Trypticase glucose yeast broth fit a four-parameter and a quadratic model, respectively. The monoclonal antibody-based ELISA developed is a rapid, sensitive and specific detection method and can be used for quantitative characterization of C. perfringens alpha-toxin.

Monoclonal Antibody Preparations for Immunotherapy of Experimental GBS Infection

2015 ◽  
pp. 254-266 ◽  
Author(s):  
A. O. Shigeoka ◽  
S. H. Pincus ◽  
N. S. Rote ◽  
D. G. Pritchard ◽  
J. I. Santos ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Antibody Preparations
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