In situ removal of ammonium ions from hybridoma cell culture media: Selection of adsorbent

1992 ◽  
Vol 6 (4) ◽  
pp. 341-346 ◽  
Author(s):  
Yeon-Ho Jeong ◽  
Shaw S. Wang
Keyword(s):  
Cell Culture ◽  
Culture Media ◽  
Media Selection ◽  
Ammonium Ions ◽  
Cell Culture Media ◽  
Hybridoma Cell Culture ◽  
Selection Of

scholarly journals Thiol-Methylsulfone Based Hydrogels: Enhanced Control on Gelation Kinetics for 3D Cell Encapsulation

2019 ◽  
Author(s):  
Julieta Paez ◽  
Aleeza Farrukh ◽  
Małgorzata K. Włodarczyk-Biegun ◽  
Aránzazu del Campo
Keyword(s):  
Cell Culture ◽  
Culture Media ◽  
Cell Encapsulation ◽  
Crosslinking Reaction ◽  
Light Activation ◽  
Cell Culture Media ◽  
Physiological Conditions ◽  
Gelation Kinetics ◽  
Homogenous Distribution ◽  
Selection Of

Hydrogels are useful temporal matrices for cell culture technologies. The successful mixing and encapsulation of cells within the gel requires the selection of efficient and cytocompatible gelation reactions occurring in the minute timescale under physiological conditions. The thiol-methylsulfonyl (MS) chemical reaction is introduced here as a novel chemistry to encapsulate cells in polymeric matrices. Thiol-MS crosslinking does not require a light activation step and can occur within the seconds-to-minutes timescale by adjusting the pH in the physiological range 8.0-6.6. This reaction is cytocompatible and the reaction product is hydrolytically stable in cell culture media up to 4 weeks. Cell encapsulation protocols enabling comfortable handling and yielding homogenous distribution of the embedded cells are described. All these features are relevant for the application of this crosslinking reaction to biomedical scenarios. Finally, this manuscript also compares the performance of thiol-MS hydrogels with the established thiol-maleimide and thiol-vinylsulfone hydrogels. The benefit of thiol-MS crosslinking in terms of control over hydrogelation kinetics is demonstrated.

scholarly journals Thiol-Methylsulfone Based Hydrogels: Enhanced Control on Gelation Kinetics for 3D Cell Encapsulation

2019 ◽  
Author(s):  
Julieta Paez ◽  
Aleeza Farrukh ◽  
Małgorzata K. Włodarczyk-Biegun ◽  
Aránzazu del Campo
Keyword(s):  
Cell Culture ◽  
Culture Media ◽  
Cell Encapsulation ◽  
Crosslinking Reaction ◽  
Light Activation ◽  
Cell Culture Media ◽  
Physiological Conditions ◽  
Gelation Kinetics ◽  
Homogenous Distribution ◽  
Selection Of

Hydrogels are useful temporal matrices for cell culture technologies. The successful mixing and encapsulation of cells within the gel requires the selection of efficient and cytocompatible gelation reactions occurring in the minute timescale under physiological conditions. The thiol-methylsulfonyl (MS) chemical reaction is introduced here as a novel chemistry to encapsulate cells in polymeric matrices. Thiol-MS crosslinking does not require a light activation step and can occur within the seconds-to-minutes timescale by adjusting the pH in the physiological range 8.0-6.6. This reaction is cytocompatible and the reaction product is hydrolytically stable in cell culture media up to 4 weeks. Cell encapsulation protocols enabling comfortable handling and yielding homogenous distribution of the embedded cells are described. All these features are relevant for the application of this crosslinking reaction to biomedical scenarios. Finally, this manuscript also compares the performance of thiol-MS hydrogels with the established thiol-maleimide and thiol-vinylsulfone hydrogels. The benefit of thiol-MS crosslinking in terms of control over hydrogelation kinetics is demonstrated.

scholarly journals In Situ Swelling Behavior of Chitosan-Polygalacturonic Acid/Hydroxyapatite Nanocomposites in Cell Culture Media

2010 ◽  
Vol 2010 ◽  
pp. 1-12 ◽  
Author(s):  
Rohit Khanna ◽  
Kalpana S. Katti ◽  
Dinesh R. Katti
Keyword(s):  
Cell Culture ◽  
Structural Integrity ◽  
Culture Media ◽  
Sample Surface ◽  
Nanocomposite Films ◽  
Apatite Formation ◽  
Polygalacturonic Acid ◽  
Cell Culture Media ◽  
Polyelectrolyte Complexes

The molecular and mechanical characteristics of in situ degradation behavior of chitosan-polygalacturonic acid/hydroxyapatite (Chi-PgA-HAP) nanocomposite films is investigated using Fourier Transform Infrared spectroscopy (FTIR), Atomic Force Microscopy (AFM), and modulus mapping techniques for up to 48 days of soaking in cell culture media. The surface molecular structure of media-soaked samples changes over the course of 48 days of soaking, as indicated by significant changes in phosphate vibrations (1200–900 ) indicating apatite formation. Chitosan-Polygalacturonic acid polyelectrolyte complexes (PECs) govern structural integrity of Chi-PgA-HAP nanocomposites and FTIR spectra indicate that PECs remain intact until 48 days of soaking. In situ AFM experiments on media-soaked samples indicate that soaking results in a change in topography and swelling proceeds differently at the initial soaking periods of about 8 days than for longer soaking. In situ modulus mapping experiments are done on soaked samples by probing 1–3 nm of surface indicating elastic moduli of 4 GPa resulting from proteins adsorbed on Chi-PgA-HAP nanocomposites. The elastic modulus decreases by 2 GPa over a long exposure to cell culture media (48 days). Thus, as water enters the Chi-PgA-HAP sample, surface molecular interactions in Chi-PgA-HAP structure occur that result in swelling, causing small changes in nanoscale mechanical properties.

Abstract 534: Chemically Defined Cell Culture Media for High Yield Differentiation of Functional Human Cardiac Myocytes

2020 ◽  
Vol 127 (Suppl_1) ◽  
Author(s):  
Davi M Lyra Leite ◽  
Paul W Burridge
Keyword(s):  
Cell Culture ◽  
Recombinant Proteins ◽  
Pluripotent Stem Cell ◽  
Culture Media ◽  
Induced Pluripotent Stem Cell ◽  
High Yield ◽  
Cell Culture Media ◽  
Basal Media ◽  
Induced Pluripotent ◽  
Selection Of

Introduction: Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) are a promising technology for regenerative medicine and pharmacology. However, existing protocols are limited due to the high costs when generating cells at scale, to low yield, and to a lack of protocol replicability. Additionally, the use of animal-derived or recombinant proteins in cell differentiation creates concerns in human compatibility and presents technical challenges due to batch-to-batch variability. Aim: Develop a protocol for functional hiPSC-CM differentiation with high yield without the use of proteins. Methods: We expanded the CDM3 protocol by testing approximately 200 combinations of cell culture media during hiPSC differentiation. The differentiation was induced with a 24h incubation in 6 μM CHIR (d0), followed by 24 h in basal media (d1) and 48h in media supplemented with AA2P and Wnt-C59 (d2-d4). From day 4 onwards, cells were kept in media supplemented with insulin and AA2P, and in some conditions with polyvinyl alcohol. On day 8, hiPSC-CMs were antibiotically selected using geneticin. Tissues were imaged on day 13 to assess myocyte morphology and contraction. Videos were scored from 0 (dead/delaminated cells) to 9 (confluent tissues beating at least 4 times in a 5 sec interval). Results: We identified 6 basal media combinations that yield confluent monolayers on day 13. Out of these, 3 conditions consistently resulted in confluent contractile tissues. Conclusions: Our data demonstrate that a protein-free high yield hiPSC-CM differentiation is possible with the appropriate selection of basal media based on cellular nutritional requirements.

Investigation of Near-Infrared Spectroscopy for Periodic Determination of Glucose in Cell Culture Media in Situ

2000 ◽  
Vol 54 (10) ◽  
pp. 1453-1457 ◽  
Author(s):  
Christopher B. Lewis ◽  
Roger J. McNichols ◽  
Ashok Gowda ◽  
Gerard L. Coté
Keyword(s):  
Cell Culture ◽  
Infrared Spectroscopy ◽  
Near Infrared Spectroscopy ◽  
Near Infrared ◽  
Culture Media ◽  
Cell Culture Media

Cell culture media: NMR approaches to evaluating quality and nutrient consumption

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
KB Killday ◽  
AS Freund ◽  
C Fischer ◽  
KL Colson
Keyword(s):  
Cell Culture ◽  
Culture Media ◽  
Cell Culture Media ◽  
Nutrient Consumption
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