Optimization of L(+)-lactic acid production employing statistical experimental design

1994 ◽  
Vol 8 (5) ◽  
pp. 325-330 ◽  
Author(s):  
M. Hujanen ◽  
Y. -Y. Linko
Keyword(s):  
Lactic Acid ◽  
Experimental Design ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Statistical Experimental Design

scholarly journals Metabolic Engineering of Lactobacillus helveticus CNRZ32 for Production of Purel-(+)-Lactic Acid

2000 ◽  
Vol 66 (9) ◽  
pp. 3835-3841 ◽  
Author(s):  
Kari Kyl�-Nikkil� ◽  
Mervi Hujanen ◽  
Matti Leisola ◽  
Airi Palva
Keyword(s):  
Lactic Acid ◽  
Structural Gene ◽  
Acid Production ◽  
Wild Type Strain ◽  
Lactic Acid Production ◽  
Gene Replacement ◽  
Lactobacillus Helveticus ◽  
Statistical Experimental Design ◽  
Wild Type ◽  
In The Wild

ABSTRACT Expression of d-(−)-lactate dehydrogenase (d-LDH) and l-(+)-LDH genes (ldhDand ldhL, respectively) and production ofd-(−)- and l-(+)-lactic acid were studied inLactobacillus helveticus CNRZ32. In order to develop a host for production of pure l-(+)-isomer of lactic acid, twoldhD-negative L. helveticus CNRZ32 strains were constructed using gene replacement. One of the strains was constructed by deleting the promoter region of the ldhD gene, and the other was constructed by replacing the structural gene ofldhD with an additional copy of the structural gene (ldhL) of l-LDH of the same species. The resulting strains were designated GRL86 and GRL89, respectively. In strain GRL89, the second copy of the ldhL structural gene was expressed under the ldhD promoter. The twod-LDH-negative strains produced onlyl-(+)-lactic acid in an amount equal to the total lactate produced by the wild type. The maximum l-LDH activity was found to be 53 and 93% higher in GRL86 and GRL89, respectively, than in the wild-type strain. Furthermore, process variables forl-(+)-lactic acid production by GRL89 were optimized using statistical experimental design and response surface methodology. The temperature and pH optima were 41�C and pH 5.9. At low pH, when the growth and lactic acid production are uncoupled, strain GRL89 produced approximately 20% more lactic acid than GRL86.

scholarly journals Experimental design for optimization of D-lactic acid production using a UV-light selected strain

2018 ◽  
Vol 5 (4) ◽  
Author(s):  
Regiane Alves de Oliveira ◽  
Luciana Fontes Coelho ◽  
Natalia Sozza Bernardi ◽  
Jonas Contiero
Keyword(s):  
Lactic Acid ◽  
Experimental Design ◽  
Acid Production ◽  
Uv Light ◽  
Lactic Acid Production

scholarly journals Protective effect of titanium tetrafluoride and silver diamine fluoride on radiation-induced dentin caries in vitro

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Beatriz Martines de Souza ◽  
Mayara Souza Silva ◽  
Aline Silva Braga ◽  
Patrícia Sanches Kerges Bueno ◽  
Paulo Sergio da Silva Santos ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Protective Effect ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Negative Control ◽  
Titanium Tetrafluoride ◽  
Silver Diamine Fluoride ◽  
Dentin Caries ◽  
Radiation Induced

AbstractThis in vitro study evaluated the protective effect of titanium tetrafluoride (TiF4) varnish and silver diamine fluoride (SDF) solution on the radiation-induced dentin caries. Bovine root dentin samples were irradiated (70 Gy) and treated as follows: (6 h): 4% TiF4 varnish; 5.42% NaF varnish; 30% SDF solution; placebo varnish; or untreated (negative control). Microcosm biofilm was produced from human dental biofilm (from patients with head-neck cancer) mixed with McBain saliva for the first 8 h. After 16 h and from day 2 to day 5, McBain saliva (0.2% sucrose) was replaced daily (37 °C, 5% CO2) (biological triplicate). Demineralization was quantified by transverse microradiography (TMR), while biofilm was analyzed by using viability, colony-forming units (CFU) counting and lactic acid production assays. The data were statistically analyzed by ANOVA (p < 0.05). TiF4 and SDF were able to reduce mineral loss compared to placebo and the negative control. TiF4 and SDF significantly reduced the biofilm viability compared to negative control. TiF4 significantly reduced the CFU count of total microorganism, while only SDF affected total streptococci and mutans streptococci counts. The varnishes induced a reduction in lactic acid production compared to the negative control. TiF4 and SDF may be good alternatives to control the development of radiation-induced dentin caries.

scholarly journals Lactic acid production ability of Lactobacillus sp. from four tropical fruits using their by-products as carbon source

Heliyon ◽  
2021 ◽  
Vol 7 (5) ◽  
pp. e07079
Author(s):  
Joel Romial Ngouénam ◽  
Chancel Hector Momo Kenfack ◽  
Edith Marius Foko Kouam ◽  
Pierre Marie Kaktcham ◽  
Rukesh Maharjan ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Carbon Source ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Tropical Fruits ◽  
By Products

γ-Valerolactone-Introduced Controlled-Isomerization of Glucose for Lactic Acid Production over Sn-Beta Catalyst

2021 ◽  
Author(s):  
Xinpeng Zhao ◽  
Zhimin Zhou ◽  
hu luo ◽  
Yanfei Zhang ◽  
Wang Liu ◽  
...  
Keyword(s):  
Density Functional Theory ◽  
Lactic Acid ◽  
Dft Calculations ◽  
Acid Production ◽  
Density Functional ◽  
Lactic Acid Production ◽  
Hydrothermal Conversion ◽  
Functional Theory ◽  
Environment Friendly

Combined experiments and density functional theory (DFT) calculations provided insights into the role of the environment-friendly γ-valerolactone (GVL) as a solvent in the hydrothermal conversion of glucose into lactic acid...

Impact of storage duration and micro-aerobic conditions on lactic acid production from food waste

2021 ◽  
Vol 323 ◽  
pp. 124618
Author(s):  
Zengshuai Zhang ◽  
Panagiotis Tsapekos ◽  
Merlin Alvarado-Morales ◽  
Irini Angelidaki
Keyword(s):  
Lactic Acid ◽  
Food Waste ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Storage Duration ◽  
Aerobic Conditions
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