Neonatal hamster molar tooth development: Extraction and characterization of amelogenins, enamelins, and soluble dentin proteins

1982 ◽  
Vol 34 (1) ◽  
pp. 86-96 ◽  
Author(s):  
D. M. Lyaruu ◽  
A. Belcourt ◽  
A. G. Fincham ◽  
J. D. Termine
Keyword(s):  
Tooth Development ◽  
Molar Tooth

Expression of neural cell-adhesion molecule mRNA during mouse molar tooth development

2002 ◽  
Vol 47 (11) ◽  
pp. 805-813 ◽  
Author(s):  
Nobuko Obara ◽  
Yuko Suzuki ◽  
Yasuko Nagai ◽  
Hiromasa Nishiyama ◽  
Itaru Mizoguchi ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Adhesion Molecule ◽  
Cell Adhesion Molecule ◽  
Neural Cell Adhesion Molecule ◽  
Tooth Development ◽  
Neural Cell ◽  
Molar Tooth ◽  
Neural Cell Adhesion

Age Distribution of Red Tree Voles in Northern Spotted Owl Pellets Estimated from Molar Tooth Development

2020 ◽  
Vol 93 (3-4) ◽  
pp. 193
Author(s):  
Chad A. Marks-Fife ◽  
Eric D. Forsman ◽  
Katie M. Dugger
Keyword(s):  
Tooth Development ◽  
Age Distribution ◽  
Molar Tooth ◽  
Spotted Owl ◽  
Northern Spotted Owl ◽  
Owl Pellets

scholarly journals Expression, localization and synthesis of small leucine-rich proteoglycans in developing mouse molar tooth germ

2020 ◽  
Vol 64 (1) ◽  
Author(s):  
Angammana Randilini ◽  
Kaoru Fujikawa ◽  
Shunichi Shibata
Keyword(s):  
Organ Culture ◽  
Developmental Stages ◽  
Tooth Development ◽  
Expression Patterns ◽  
Tooth Germ ◽  
Molar Tooth ◽  
Cervical Region ◽  
Metabolic Labeling ◽  
Tooth Formation ◽  
Tooth Germs

The gene expression and protein synthesis of small leucine-rich proteoglycans (SLRPs), including decorin, biglycan, fibromodulin, and lumican, was analyzed in the context of the hypothesis that they are closely related to tooth formation. In situ hybridization, immunohistochemistry, and organ culture with metabolic labeling of [35S] were carried out in mouse first molar tooth germs of different developmental stages using ICR mice at embryonic day (E) 13.5 to postnatal day (P) 7.0. At the bud and cap stage, decorin mRNA was expressed only in the surrounding mesenchyme, but not within the tooth germ. Biglycan mRNA was then expressed in the condensing mesenchyme and the dental papilla of the tooth germ. At the apposition stage (late bell stage), both decorin and biglycan mRNA were expressed in odontoblasts, resulting in a switch of the pattern of expression within the different stages of odontoblast differentiation. Decorin mRNA was expressed earlier in newly differentiating odontoblasts than biglycan. With odontoblast maturation and dentin formation, decorin mRNA expression was diminished and localized to the newly differentiating odontoblasts at the cervical region. Simultaneously, biglycan mRNA took over and extended its expression throughout the new and mature odontoblasts. Both mRNAs were expressed in the dental pulp underlying the respective odontoblasts. At P7.0, both mRNAs were weakly expressed but maintained their spatial expression patterns. Immunostaining showed that biglycan was localized in the dental papillae and pulp. In addition, all four SLRPs showed clear immunostaining in predentin, although the expressions of fibromodulin and lumican mRNAs were not identified in the tooth germs examined. The organ culture data obtained supported the histological findings that biglycan is more predominant than decorin at the apposition stage. These results were used to identify biglycan as the principal molecule among the SLRPs investigated. Our findings indicate that decorin and biglycan show spatial and temporal differential expressions and play their own tissue-specific roles in tooth development.

An Antisera for the Fluorescent Labeling of Mouse Amelogenesis

1979 ◽  
Vol 58 (2_suppl) ◽  
pp. 1008-1009 ◽  
Author(s):  
H. Hyatt-Fischer ◽  
J. Chrispens ◽  
D. O'Keefe ◽  
H.C. Slavkin
Keyword(s):  
Extracellular Matrix ◽  
New Zealand ◽  
Tooth Development ◽  
Fluorescent Labeling ◽  
Molar Tooth ◽  
Maxillary Incisor ◽  
Enamel Matrix ◽  
Immunofluorescent Microscopy ◽  
New Zealand White Rabbits ◽  
Indirect Immunofluorescent

Embryonic mammalian enamel extracellular matrix is immunogenic. Antisera has been produced in New Zealand white rabbits using 5-day-old (postnatal) C57B1/6J mandibular and maxillary incisor and molar tooth organs as immunogens. The expression of secretory amelogenesis in mouse molar tooth organs was studied from the "cap stage" (circa 17-day fetus) to the fifth day of postnatal odontogenesis using indirect immunofluorescent microscopy. The specificity of the antisera for enamel matrix secretion was unequivocal. Secretory amelogenesis was observed in molar tooth organs as early as day-2 postnatal age. These reagents and methods provide a significant strategy in studies of epithelial-mesenchymal interactions during tooth development.

scholarly journals Anomalous incisor morphology indicates tissue-specific roles for Tfap2a and Tfap2b in tooth development

2020 ◽  
Author(s):  
Emily D. Woodruff ◽  
Galaxy C. Gutierrez ◽  
Eric Van Otterloo ◽  
Trevor Williams ◽  
Martin J. Cohn
Keyword(s):  
Transcription Factors ◽  
Neural Crest ◽  
Tooth Development ◽  
Molar Tooth ◽  
Fine Tuning ◽  
List Type ◽  
Molecular Signaling ◽  
Tissue Specific ◽  
Essential Components ◽  
Tooth Germs

AbstractMice possess two types of teeth that differ in their cusp patterns; incisors have one cusp and molars have multiple cusps. The patterning of these two types of teeth relies on fine-tuning of the reciprocal molecular signaling between dental epithelial and mesenchymal tissues during embryonic development. Here we show that the incisors are populated only at early time points by the neural crest, whereas the molars continue to receive contributions at later stages, revealing a temporal difference that could alter epithelial-mesenchymal signaling dynamics between these two types of teeth. The AP-2 transcription factors, particularly Tfap2a and Tfap2b, are essential components of such epithelial-mesenchymal signaling interactions that coordinate craniofacial development in mice and other mammals, but little is known about their roles in the regulation of tooth development and shape. We demonstrate that incisors and molars differ in their temporal and spatial expression of Tfap2a and Tfap2b; in particular, at the bud stage, Tfap2a is expressed in both the epithelium and mesenchyme of the incisors and molars but expression of Tfap2b is restricted to the mesenchyme of the molars. Tissue-specific deletions show that loss of the epithelial domain of Tfap2a and Tfap2b affects the number and spatial arrangement of the incisors, notably resulting in duplicated lower incisors. In contrast, deletion of these two genes in the mesenchymal domain has little effect on tooth development. Collectively these results implicate epithelial expression of Tfap2a and Tfap2b in dorsal-ventral patterning of the incisors and suggest that these genes contribute to morphological differences between anterior (incisor) and posterior (molar) teeth within the mammalian dentition.HighlightsLate-migrating cranial neural crest cells contribute extensively to the developing molar tooth germs but minimally to the incisors.During tooth development, transcription factors Tfap2a and Tfap2b are expressed in spatially and temporally dynamic patterns and differ between incisor and molar tooth germs.Epithelial expression of Tfap2a and Tfap2b is necessary for incisor development, but mesenchymal expression of these genes is not required.

Msx1 controls inductive signaling in mammalian tooth morphogenesis

Development ◽  
1996 ◽  
Vol 122 (10) ◽  
pp. 3035-3044 ◽  
Author(s):  
Y. Chen ◽  
M. Bei ◽  
I. Woo ◽  
I. Satokata ◽  
R. Maas
Keyword(s):  
Matrix Protein ◽  
Tooth Development ◽  
Molar Tooth ◽  
Extracellular Matrix Protein ◽  
Msx Genes ◽  
Tooth Morphogenesis ◽  
Tooth Germs ◽  
Vertebrate Organogenesis ◽  
Inductive Signaling

Members of the Msx homeobox family are thought to play important roles in inductive tissue interactions during vertebrate organogenesis, but their precise developmental function has been unclear. Mice deficient for Msx1 exhibit defects in craniofacial development and a failure of tooth morphogenesis, with an arrest in molar tooth development at the E13.5 bud stage. Because of its potential for experimental manipulation, the murine molar tooth germ provides a powerful system for studying the role of Msx genes in inductive signaling during organogenesis. To further analyze the role of Msx1 in regulating epithelial-mesenchymal interactions during tooth morphogenesis, we have examined the expression of several potential Msx1 downstream genes in Msx1 mutant tooth germs and we have performed functional experiments designed to order these genes into a pathway. Our results show that expression of Bone Morphogenetic Protein 4 (BMP4), the HMG box gene Lef1 and the heparan sulfate proteoglycan syndecan-1 is specifically reduced in Msx1 mutant dental mesenchyme, while expression of the extracellular matrix protein tenascin is unaffected. BMP4 soaked beads can induce Bmp4 and Lef1 expression in explanted wild-type dental mesenchymes, but only Lef1 expression in Msx1 mutant dental mesenchyme. We thus conclude that epithelial BMP4 induces its own expression in dental mesenchyme in a manner that requires Msx1. In turn, we show that addition of BMP4 to Msx1 deficient tooth germs bypasses the requirement for Msx1 and rescues epithelial development from the bud stage to the E14.5 cap stage. Lastly, we show that FGFs induce syndecan-1 expression in dental mesenchyme in a manner that also requires Msx-1. These results integrate Msx1 into a regulatory hierarchy in early tooth morphogenesis and demonstrate that Msx1 is not only expressed in dental mesenchyme in response to epithelial signals, but also in turn regulates the reciprocal expression of inductive signals in the mesenchyme which then act back upon the dental epithelium. We propose that Msx genes function repetitively during vertebrate organogenesis to permit inductive signaling to occur back and forth between tissue layers.

The effects of ascorbic acid deficiency on collagen synthesis by mouse molar tooth germs in organ culture

Development ◽  
1977 ◽  
Vol 37 (1) ◽  
pp. 49-57
Author(s):  
John R. Schiltz ◽  
Joel Rosenbloom ◽  
Gordon E. Levenson
Keyword(s):  
Ascorbic Acid ◽  
Organ Culture ◽  
Culture Medium ◽  
Large Fraction ◽  
Culture Conditions ◽  
Molar Tooth ◽  
Second Molar ◽  
Acid Deficiency ◽  
Tooth Germs

Second molar tooth germs from 2-day-old Swiss-Webster mice, grown in organ culture for 7 days in ascorbic-acid-deficient medium, synthesized about 65 % as much protein (measured by incorporation of [14C]proline during a 24-h pulse) as did ascorbic-acid-supplemented controls. The newly synthesized proteins from ascorbic-acid-deficient cultures contained only about 7% of the hydroxyproline content of the controls. Collagenase digestion of the newly synthesized proteins showed that collagen comprised the same fraction of the total protein synthesized under both culture conditions. This result indicates that the ascorbatedeficient cultures made significant quantities of underhydroxylated collagen. Partial characterization of the collagen alpha chains on carboxymethyl cellulose columns showed an α1/α2 ratio of about 5, suggesting that at least two different species of collagen were synthesized. The α1/α2 ratio of the chains recovered from the ascorbate-deficient cultures was also about 5 but the chains were slightly underhydroxylated and the total amount of these chains which could be identified accounted for only a small fraction of the total collagen which was synthesized. A large fraction of the synthesized collagenous protein was found in the culture medium, mostly in the form of lower molecular weight peptides. It is concluded that most of the collagen which is synthesized by ascorbate-deficient tooth-bud cultures is not utilized by the component tissues, but is probably degraded and released into the medium.

scholarly journals Characterization of stem cells from the pulp of unerupted third molar tooth

2014 ◽  
Vol 25 (1) ◽  
pp. 14 ◽  
Author(s):  
Ahmad Monabati ◽  
Yasamin Hadaegh ◽  
Mahnaz Niknam ◽  
Armin Attar ◽  
MohsenKhosravi Maharlooei ◽  
...  
Keyword(s):  
Stem Cells ◽  
Third Molar ◽  
Molar Tooth
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