Magnesium ion catalyzed P-N bond hydrolysis in imidazolide activated nucleotides. Relevance to template-directed synthesis of polynucleotides

1989 ◽  
Vol 19 (3-5) ◽  
pp. 353-353
Author(s):  
Anastassia Kanavarioti ◽  
Claude F. Bernasconi ◽  
Donald L. Doodokyan ◽  
Diann Alberas
Keyword(s):  
Magnesium Ion ◽  
Directed Synthesis

Magnesium ion catalyzed phosphorus-nitrogen bond hydrolysis in imidazolide-activated nucleotides. Relevance to template-directed synthesis of polynucleotides

1989 ◽  
Vol 111 (18) ◽  
pp. 7247-7257 ◽  
Author(s):  
Anastassia Kanavarioti ◽  
Claude F. Bernasconi ◽  
Donald L. Doodokyan ◽  
Diann J. Alberas
Keyword(s):  
Magnesium Ion ◽  
Directed Synthesis ◽  
Nitrogen Bond

Stability of B12 (CN)12 2− : Implications for Lithium and Magnesium Ion Batteries

2016 ◽  
Vol 128 (11) ◽  
pp. 3768-3772 ◽  
Author(s):  
Hongmin Zhao ◽  
Jian Zhou ◽  
Puru Jena
Keyword(s):  
Magnesium Ion ◽  
Magnesium Ion Batteries

scholarly journals Base-Pair Opening Dynamics Study of Fluoride Riboswitch in the Bacillus cereus CrcB Gene

2021 ◽  
Vol 22 (6) ◽  
pp. 3234
Author(s):  
Juhyun Lee ◽  
Si-Eun Sung ◽  
Janghyun Lee ◽  
Jin Young Kang ◽  
Joon-Hwa Lee ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Regulation ◽  
Nmr Spectroscopy ◽  
Bacillus Cereus ◽  
Base Pair ◽  
Noncoding Rna ◽  
Hydrogen Exchange ◽  
Magnesium Ion ◽  
Fluoride Ion ◽  
Base Pair Opening

Riboswitches are segments of noncoding RNA that bind with metabolites, resulting in a change in gene expression. To understand the molecular mechanism of gene regulation in a fluoride riboswitch, a base-pair opening dynamics study was performed with and without ligands using the Bacillus cereus fluoride riboswitch. We demonstrate that the structural stability of the fluoride riboswitch is caused by two steps depending on ligands. Upon binding of a magnesium ion, significant changes in a conformation of the riboswitch occur, resulting in the greatest increase in their stability and changes in dynamics by a fluoride ion. Examining hydrogen exchange dynamics through NMR spectroscopy, we reveal that the stabilization of the U45·A37 base-pair due to the binding of the fluoride ion, by changing the dynamics while maintaining the structure, results in transcription regulation. Our results demonstrate that the opening dynamics and stabilities of a fluoride riboswitch in different ion states are essential for the genetic switching mechanism.

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