Altering the dissolved oxygen tension in tissue culture media

1991 ◽  
Vol 13 (4) ◽  
pp. 241-245 ◽  
Author(s):  
Loraine H. Anderson ◽  
Frank J. Roberts ◽  
Bernard Wilson ◽  
William J. Mehm
Keyword(s):  
Tissue Culture ◽  
Dissolved Oxygen ◽  
Oxygen Tension ◽  
Culture Media ◽  
Dissolved Oxygen Tension ◽  
Tissue Culture Media

The influence of dissolved oxygen tension on the metabolic activity of an immobilized hybridoma population

1993 ◽  
Vol 13 (1) ◽  
pp. 29-39 ◽  
Author(s):  
J. Th�mmes ◽  
J. G�tgens ◽  
M. Biselli ◽  
P. W. Runstadler ◽  
C. Wandrey
Keyword(s):  
Dissolved Oxygen ◽  
Oxygen Tension ◽  
Metabolic Activity ◽  
Dissolved Oxygen Tension

Fractional Factorial Design for Optimization of the SELDI Protocol for Human Adipose Tissue Culture Media

2007 ◽  
Vol 23 (1) ◽  
pp. 217-224 ◽  
Author(s):  
E. Szalowska ◽  
S.A.F.T. vanHijum ◽  
H. Roelofsen ◽  
A. Hoek ◽  
R.J. Vonk ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Adipose Tissue ◽  
Factorial Design ◽  
Fractional Factorial Design ◽  
Culture Media ◽  
Human Adipose Tissue ◽  
Fractional Factorial ◽  
Tissue Culture Media

scholarly journals Reversible oxidant-induced increases in albumin transfer across cultured endothelium: alterations in cell shape and calcium homeostasis

Blood ◽  
1985 ◽  
Vol 65 (3) ◽  
pp. 605-614
Author(s):  
DM Shasby ◽  
SE Lind ◽  
SS Shasby ◽  
JC Goldsmith ◽  
GW Hunninghake
Keyword(s):  
Tissue Culture ◽  
Xanthine Oxidase ◽  
Calcium Homeostasis ◽  
Actin Filaments ◽  
Cell Shape ◽  
Culture Media ◽  
Extracellular Calcium ◽  
Lanthanum Chloride ◽  
Tissue Culture Media ◽  
Induced Changes

To determine whether reactive oxygen molecules could directly and reversibly increase the transfer of albumin across an endothelial barrier, we measured albumin transfer across monolayers of endothelium cultured on micropore filters before and after exposure to xanthine and xanthine oxidase. Xanthine and xanthine oxidase increased endothelial albumin transfer in a dose-dependent fashion. Parallel phase contrast and fluorescence microscopy demonstrated retraction of adjacent cells from one another and disruption of the actin filaments. The oxidant- induced increases in albumin transfer and changes in cell shape were reversed by removing xanthine oxidase and then incubating the monolayers for 3 1/2 hours in tissue culture media enriched with fetal bovine serum. However, incubation in tissue culture media without serum resulted in progressive injury and cell death. Hence, the brief exposure to oxidants initiated a progressive injury process that was reversed by incubation in serum. Because intracellular and extracellular calcium are important determinants of cell shape, and because some oxidized membrane lipids act as calcium ionophores, we asked whether oxidants altered endothelial calcium homeostasis. Xanthine-xanthine oxidase increased release of 45Ca++ from preloaded cells. The calcium antagonist lanthanum chloride prevented xanthine- xanthine oxidase increases in endothelial albumin transfer and prevented the changes in cell shape; chelation of extracellular calcium inhibited lysis of endothelium by xanthine-xanthine oxidase; and the calcium ionophore A23187 increased endothelial albumin transfer and mimicked the oxidant-induced changes in cell shape. Lanthanum chloride inhibited these effects of A23187. These data suggest that oxygen radicals can reversibly increase endothelial permeability to macromolecules, that this is associated with reversible changes in endothelial cell shape and actin filaments, and that the changes in cell shape are related to oxidant-induced changes in endothelial calcium homeostasis.

The Influence of PH and Dissolved Oxygen Tension (DOT) on Mycelium Growth and Cellulase Production by Trichoderma Reesei

2011 ◽  
Vol 236-238 ◽  
pp. 1005-1013
Author(s):  
Zhi Xi Hang ◽  
Qing Long Rao ◽  
Shi Yuan Yu
Keyword(s):  
Dissolved Oxygen ◽  
Trichoderma Reesei ◽  
Oxygen Tension ◽  
Filter Paper ◽  
Mass Concentration ◽  
Cellulase Production ◽  
Mycelium Growth ◽  
Dissolved Oxygen Tension ◽  
Influence Of Ph ◽  
Filter Paper Activity

The influence of pH and dissolved oxygen tension (DOT) on mycelium growth and cellulase production by Trichoderma reesei was studied in this paper. The experiments were carried out with a cellulose of 10 g/l in a 10 L steam sterilizable bioreactor. The results have shown that H+ concentration was highly fluctuated in the growing and metabolizing periods of mycelium, which went against mycelium growth and cellulase production. Controlling pH to 4.8 was favorable to mycelium growth and cellulase production; the maximum mycelium mass concentration was increased from 2.60 g/l to 2.77 g/l; the maximum filter paper activity was raised from 1.87 IU/ml to 2.79 IU/ml. Meanwhile, the growth and metabolism of mycelium demand an appropriate dissolved oxygen tension (DOT). When the velocity of aeration was increased from 0.4 to 0.5vvm to improve the condition of dissolving oxygen, the mycelium mass concentration was increased from 2.77 g/l to 2.98g/l, and the filter paper activity was raised from 2.79 IU/ml to 2.98 IU/ml.

Retinoic acid increases the sensitivity of the rat embryo fibroblast transformation assay

1988 ◽  
Vol 8 (4) ◽  
pp. 1845-1848
Author(s):  
T D Halazonetis ◽  
C Daugherty ◽  
P Leder
Keyword(s):  
Tissue Culture ◽  
Retinoic Acid ◽  
Culture Media ◽  
Rat Embryo ◽  
Transformation Assay ◽  
Tissue Culture Media ◽  
Focus Assay ◽  
Fibroblast Transformation

The rat embryo fibroblast focus assay is used to evaluate the transforming potential of several oncogenes. The sensitivity of this assay increased fivefold when retinoic acid was added to tissue culture media. Retinoic acid probably acts by selectively inhibiting the proliferation of nontransformed cells.

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