Procedure for concentration and conditioning of hybridoma cell culture medium for monoclonal antibody purification

1991 ◽  
Vol 13 (1) ◽  
pp. 49-51
Author(s):  
K. Veeraragavan ◽  
A. Bernier ◽  
M. Oliver ◽  
E. Braendli
Keyword(s):  
Cell Culture ◽  
Monoclonal Antibody ◽  
Culture Medium ◽  
Cell Culture Medium ◽  
Antibody Purification ◽  
Hybridoma Cell Culture

Cell culture medium supplemented with taurine decreases basic charge variant levels of a monoclonal antibody

2018 ◽  
Vol 40 (11-12) ◽  
pp. 1487-1493 ◽  
Author(s):  
Mengjuan Liu ◽  
Jiaqi Wang ◽  
Hongping Tang ◽  
Li Fan ◽  
Liang Zhao ◽  
...  
Keyword(s):  
Cell Culture ◽  
Monoclonal Antibody ◽  
Culture Medium ◽  
Cell Culture Medium

Effect of cell culture medium components on color of formulated monoclonal antibody drug substance

2013 ◽  
Vol 29 (5) ◽  
pp. 1270-1277 ◽  
Author(s):  
Natarajan Vijayasankaran ◽  
Sharat Varma ◽  
Yi Yang ◽  
Melissa Mun ◽  
Silvana Arevalo ◽  
...  
Keyword(s):  
Cell Culture ◽  
Monoclonal Antibody ◽  
Culture Medium ◽  
Drug Substance ◽  
Cell Culture Medium ◽  
Medium Components ◽  
Antibody Drug

A Structure-Function Study of the Activity of Stem Cell Factor in Stimulating the Expansion of Cord Blood CD34+ Cells

Blood ◽  
2014 ◽  
Vol 124 (21) ◽  
pp. 3501-3501
Author(s):  
Bin Shen ◽  
Wenhong Jiang ◽  
Jie Fan ◽  
Wei Dai ◽  
Xinxin Ding ◽  
...  
Keyword(s):  
Cell Culture ◽  
Monoclonal Antibody ◽  
Stem Cell ◽  
Stem Cell Factor ◽  
Culture Medium ◽  
Cell Number ◽  
Full Length ◽  
Cell Culture Medium ◽  
Cd34 Cells

Abstract Stem cell factor is one of the most important growth factors for human hematopoietic stem cells (HSC). Recombinant human stem cell factor (rhSCF) can stimulate HSC expansion and regeneration in vitro, when it is used in combination with other cytokines like Flt-3L and TPO. However, the specific structural region(s) of the rhSCF protein that are critical for its function in HSC expansion are still unknown. Few studies have addressed this problem, to date. We have recently reported the production of a novel monoclonal antibody (named 23C8) against rhSCF, and the demonstration that 23C8 could inhibit the ability of rhSCF to enhance HSC expansion. Here, we report the identification of a short polypeptide from rhSCF that contains the epitope for binding to 23C8, and, like the full-length rhSCF, is able to stimulate the expansion of umbilical cord blood (UCB)-derived CD34+ cells. Twelve short polypeptides were designed and synthesized, which cover the full length of rhSCF, with 3-5 amino acids overlaps. 23C8 was collected from hybridoma cell culture medium and further purified using protein G affinity chromatography. ELISA was used to identify the polypeptide(s) that positively react with 23C8 among all the synthesized polypeptides. In addition, the effects of the synthetic polypeptides on human HSC expansion capacity were evaluated by supplementing the cell culture medium with 100 ng/ml of a given polypeptide. Total cell number and CD34+ cell number of each group were monitored on day 6. Our novel anti-SCF monoclonal antibody (23C8) partially blocked SCF’s function in human UCB CD34+ cell expansion. Of all the polypeptides analyzed, only one, named P0, corresponding to the SCF protein sequence at residues 40 to 57, was recognized by 23C8 during ELISA. P0, like the full-length rhSCF, enhanced expansion of CD34+ cells derived from human UCB. P0 addition increased the numbers of total nucleated cells and CD34+ cells by 10.58±0.86 and 4.63±0.43 folds, respectively. For comparison, the extents of increases in cell numbers in the vehicle control group was 3.15±0.99 fold (total nucleated cells) and 1.07±0.11 fold (CD34+ cells), respectively. Residues 40-57 of hrSCF comprise a critical functional region for its ability to enhance expansion of human UCB CD34+ cells in vitro. The short P0 peptide is a potential candidate for development as a synthetic substitute for rhSCF in clinic applications. Disclosures Jiang: Biopharmagen.corp: Employment. Jiang:Biopharmagen.corp: Employment.

scholarly journals A metabolic network-based approach for developing feeding strategies for CHO cells to increase monoclonal antibody production

2019 ◽  
Author(s):  
Hamideh Fouladiha ◽  
Sayed-Amir Marashi ◽  
Fatemeh Torkashvand ◽  
Fereidoun Mahboudi ◽  
Nathan E. Lewis ◽  
...  
Keyword(s):  
Cell Culture ◽  
Monoclonal Antibody ◽  
Metabolic Network ◽  
Cho Cells ◽  
Rational Design ◽  
Culture Medium ◽  
Feeding Strategies ◽  
Cell Culture Medium ◽  
Bioprocess Optimization ◽  
A Genome

AbstractChinese hamster ovary (CHO) cells are the main workhorse in the biopharmaceutical industry for the production of recombinant proteins, such as monoclonal antibodies. To date, a variety of metabolic engineering approaches have been used to improve the productivity of CHO cells. While genetic manipulations are potentially laborious in mammalian cells, rational design of CHO cell culture medium or efficient fed-batch strategies are more popular approaches for bioprocess optimization. In this study, a genome-scale metabolic network model of CHO cells was used to design feeding strategies for CHO cells to improve monoclonal antibody (mAb) production. A number of metabolites, including threonine and arachidonate, were suggested by the model to be added into cell culture medium. The designed composition has been experimentally validated, and then optimized, using design of experiment methods. About a two-fold increase in the total mAb expression has been observed using this strategy. Our approach can be used in similar bioprocess optimization problems, in order to suggest new ways of increasing production in different cell factories.

Effect of cell culture medium additives on color and acidic charge variants of a monoclonal antibody

2018 ◽  
Vol 34 (5) ◽  
pp. 1298-1307 ◽  
Author(s):  
Natarajan Vijayasankaran ◽  
Sharat Varma ◽  
Yi Yang ◽  
Steven Meier ◽  
Robert Kiss
Keyword(s):  
Cell Culture ◽  
Monoclonal Antibody ◽  
Culture Medium ◽  
Cell Culture Medium ◽  
Charge Variants

3-iodothyronamine (T1AM) is taken up and rapidly metabolized in cell culture medium only at low concentration

2020 ◽  
Author(s):  
Federica Saponaro ◽  
Marco Borsò ◽  
Sara Verlotta ◽  
Lavinia Bandini ◽  
Alessandro Saba ◽  
...  
Keyword(s):  
Cell Culture ◽  
Culture Medium ◽  
Cell Culture Medium ◽  
Low Concentration

Effect of Atmospheric-Pressure Helium Plasma Jet on Cell Culture Medium

2013 ◽  
Vol 133 (5) ◽  
pp. 278-285
Author(s):  
Norimitsu Takamura ◽  
Douyan Wang ◽  
Takao Satoh ◽  
Takao Namihira ◽  
Hisato Saitoh ◽  
...  
Keyword(s):  
Cell Culture ◽  
Atmospheric Pressure ◽  
Culture Medium ◽  
Plasma Jet ◽  
Helium Plasma ◽  
Cell Culture Medium
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