Entomopathogenic nematodes for biological control of the leafminer,Liriomyza trifolii (Dipt.: Agromyzidae)

Entomophaga ◽  
1993 ◽  
Vol 38 (3) ◽  
pp. 359-369 ◽  
Author(s):  
A. H. Hara ◽  
H. K. Kaya ◽  
R. Gaugler ◽  
L. M. Lebeck ◽  
C. L. Mello
Keyword(s):  
Biological Control ◽  
Entomopathogenic Nematodes ◽  
Liriomyza Trifolii

Effectiveness of Entomopathogenic Nematodes Against Ceratitis capitata (Diptera: Tephritidae) Pupae and Nematode Compatibility with Chemical Insecticides

2021 ◽  
Author(s):  
Maguintontz Cedney Jean-Baptiste ◽  
Andressa Lima de Brida ◽  
Daniel Bernardi ◽  
Sérgio da Costa Dias ◽  
Juliano de Bastos Pazini ◽  
...  
Keyword(s):  
Biological Control ◽  
Entomopathogenic Nematodes ◽  
Ceratitis Capitata ◽  
Integrated Management ◽  
Fruit Fly ◽  
Steinernema Carpocapsae ◽  
Mediterranean Fruit Fly ◽  
Fruit Crops ◽  
Class 1 ◽  
Pupal Mortality

Abstract The Mediterranean fruit fly Ceratitis capitata (Wiedemann, 1824) (Diptera: Tephritidae) is among the main pests of fruit crops worldwide. Biological control using entomopathogenic nematodes (EPNs) may be an alternative to suppress populations of this pest. Thus, the aim of this study was to evaluate the pathogenicity and virulence of six EPN isolates (Heterorhabditis bacteriophora HB, H. amazonensis IBCB-n24, Steinernema carpocapsae IBCB-n02, S. rarum PAM-25, S. glaseri IBCB-n47, and S. brazilense IBCB-n06) against C. capitata pupae. The compatibility of EPNs with different chemical insecticides that are registered for management of C. capitata was also assessed. Isolates of H. bacteriophora HB and S. brazilense IBCB-n06 at a concentration of 1,000 infective juveniles (IJ)/ml proved to be most pathogenic to C. capitata (70 and 80% mortality, respectively). In contrast, the isolates H. amazonensis IBCB-n24, Steinernema carpocapsae IBCB-n02, S. rarum PAM-25, S. glaseri IBCB-n47 provided pupal mortality of less than 60%. Bioassays to determine lethal concentrations indicated that concentrations of 600 IJ/ml (H. bacteriophora HB) and 1,000 IJ/ml (S. brazilense IBCB-n06) showed the highest virulence against C. capitata pupae. In contrast, the highest numbers of IJs emerged at concentrations of 1,200 and 200 IJ/ml. In compatibility bioassays, malathion, spinetoram, phosmet, acetamiprid, and novaluron were considered compatible with and harmless (Class 1) to H. bacteriophora HB and S. brazilense IBCB-n06, according to IOBC/WPRS. This information is important for implementing integrated management programs for C. capitata, using biological control with EPNs, whether alone or in combination with chemical insecticides.

scholarly journals Virulence of entomopathogenic nematodes (Rhabditida: Steinernematidae and Heterorhabditidae) for the control of Diabrotica speciosa germar (coleoptera: chrysomelidae)

2011 ◽  
Vol 35 (6) ◽  
pp. 1149-1156 ◽  
Author(s):  
Viviane Santos ◽  
Alcides Moino Junior ◽  
Vanessa Andaló ◽  
Camila Costa Moreira ◽  
Ricardo Alves de Olinda
Keyword(s):  
Biological Control ◽  
Entomopathogenic Nematodes ◽  
Entomopathogenic Nematode ◽  
Larval Mortality ◽  
Steinernema Glaseri ◽  
Soil Insects ◽  
Diabrotica Speciosa ◽  
Pupal Mortality

Entomopathogenic nematodes (EPNs) are used in biological control of soil insects and show promise in the control of D. speciosa. The objective of this work was to evaluate the potential of native and exotic entomopathogenic nematode isolates in the control of D. speciosa under laboratory and greenhouse conditions. Results showed that all of EPNs caused larval mortality. The most virulent were Heterorhabditis sp. RSC01 (94%), Steinernema glaseri (84%), Heterorhabditis sp. JPM04 (82%) and Heterorhabditis amazonensis RSC05 (78%). There was no effect of the Heterorhabditis sp. RSC01 and S. glaseri isolates on eggs. The maximum mortality of D. speciosa larvae by Heterorhabditis sp. RSC01 was observed at a concentration of 300 IJ/ insect, while by S. glaseri observed the highest mortality at the concentration of 200 IJ/ insect. The Heterorhabditis sp. RSC01 isolate caused over 80% pupal mortality at a concentration of 250 IJ/insect. The virulence of Heterorhabditis sp. RSC01 and S. glaseri was affected by temperature. The Heterorhabditis sp. RSC01 isolate caused reduction in larva survival under greenhouse conditions at all of the tested concentrations and there was no difference in mortality among different concentrations of infectid juveniles.

scholarly journals Biological activity of entomopathogenic nematodes on Anastrepha fraterculus (Diptera: Tephritidae)

2021 ◽  
Vol 37 ◽  
pp. e37047
Author(s):  
Sandra Mara Chaneiko ◽  
Andressa Lima de Brida ◽  
Daniel Bernardi ◽  
Luis Garrigós Leite ◽  
Flávio Roberto Mello Garcia
Keyword(s):  
Biological Control ◽  
Biological Activity ◽  
Maximum Concentration ◽  
Entomopathogenic Nematodes ◽  
Steinernema Carpocapsae ◽  
Steinernema Feltiae ◽  
Anastrepha Fraterculus ◽  
Pupal Mortality ◽  
Control Tool

Anastrepha fraterculus (Wiedemann) is one of the main pests of fruit farming, and entomopathogenic nematodes (EPNs) represent an important control tool of this species. Thus, the objective of this study was to evaluate the biological activity of different isolate against A. fraterculus larvae and adults. Bioassays were performed using a suspension of three isolates of Heterorhabditis amazonensis IBCB 24, Steinernema carpocapsae IBCB 02 and Steinernema feltiae IBCB 47 at six concentrations (control - without nematodes), 50, 150, 300, 500, 1000 and 1500 infective juveniles (IJs)/mL of water per 3º instar larvae. It was verified the susceptibility of larvae of A. fraterculus to isolates of EPNs and a significant increase of the pupal mortality in the function of the concentration of IJs inoculated by larva (above 75%). After the dissection of pupae and adults of A. fraterculus from infected larvae, the concentration of 1500 IJs/mL of EPNs provided the highest rate of multiplication of IJs by insect, equating to maximum concentration tested 1500 IJs/mL. Adults of A. fraterculus from larvae infected with EPNs longevity of five days, being less than adults from uninfected larvae by IJs (135 days). H. amazonensis IBCB 24, S. carpocapsae IBCB 02, and S. feltiae IBCB 47 proved to be promising as agents of biological control of A. fraterculus.

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