Induction of haploid plants by anther culture in tuber-bearing species and interspecific hybrids ofSolanum

1975 ◽  
Vol 18 (1) ◽  
pp. 133-140 ◽  
Author(s):  
Yukio Irikura
Keyword(s):  
Anther Culture ◽  
Interspecific Hybrids ◽  
Haploid Plants

HAPLOID PLANTS (2n = 21) FROM in vitro ANTHER CULTURE OF TRITICUM AESTIVUM

1974 ◽  
Vol 16 (3) ◽  
pp. 697-700 ◽  
Author(s):  
Iris L. Craig
Keyword(s):  
Triticum Aestivum ◽  
Anther Culture ◽  
Triticum Aestivum L ◽  
Haploid Plants

Nine haploids (2n = 21) of Triticum aestivum L. (cv. Pitic–62) were obtained by in vitro anther culture, utilizing the medium described by Ouyang et al. (1973).

Selection of Interspecific Hybrids for Anther Culture of Indica Rice

2006 ◽  
Vol 86 (2) ◽  
pp. 271-277 ◽  
Author(s):  
T. He ◽  
Y. Yang ◽  
S. B. Tu ◽  
M. Q. Yu ◽  
X. F. Li
Keyword(s):  
Anther Culture ◽  
Interspecific Hybrids ◽  
Indica Rice ◽  
Selection Of

scholarly journals Anther Culture in Nicotiana and the Characteristics of the Haploid Plants

1973 ◽  
Vol 23 (2) ◽  
pp. 71-78 ◽  
Author(s):  
Akio Nakamura ◽  
Ryuzo Itagaki
Keyword(s):  
Anther Culture ◽  
Haploid Plants

scholarly journals Regenerasi dan Aklimatisasi Kultur Antera Enam Persilangan F1 Padi Sawah

2016 ◽  
Vol 44 (2) ◽  
pp. 133
Author(s):  
Cucu Gunarsih ◽  
Bambang Sapta Purwoko ◽  
Iswari Saraswati Dewi ◽  
Dan Muhamad Syukur
Keyword(s):  
Plant Regeneration ◽  
Anther Culture ◽  
Doubled Haploid ◽  
Callus Induction ◽  
Rainfed Rice ◽  
Randomized Design ◽  
Doubled Haploid Plants ◽  
Completely Randomized Design ◽  
N6 Medium ◽  
Haploid Plants

ABSTRACT<br /><br />The breeding of rainfed rice tolerant to drought can be accomplished using anther culture. The objectives of this research were to determine regeneration abilities of six F1 anther culture and its acclimatization ability. The experiment was arranged in completely randomized design with 14 replications. The treatments consisted of six F1 derived from crossing:  INPARI 18 x IR83140-B-11-B (G1), INPARI 18 x B12825E-TB-1-25 (G2), INPARI 18 x IR87705-14-11-B-SKI-12 (G3), INPARI 22 x IR83140-B-11-B (G4), Bio-R81 x O18b-1 (G5), Bio-R82-2 x O18b-1 (G6). Media for callus induction was based on N6 medium + 2.0 mg L-1 NAA + 0.5 mg L-1 kinetin + 1.0 mM putresin + 60 g L-1 sucrosa, media for regeneration was based on MS + 0.5 mg L-1 NAA + 2.0 mg L-1 kinetin + 1.0 mM  putresin, and media for rooting was based on  MS + 0.5 mg L-1 IBA + 30 g L-1 sucrosa. The result indicated that all six F1 had different ability in anther culture. Bio-R82-2 x O18-b1 (G6) and  Bio-R81 x O18-b1 (G5) F1 genotype had good response both of callus induction and plant regeneration. These two F1 genotypes also gave the highest ratio of green planlet production to number of anther inoculated (GP:AI) were 5.50% and 4.65%,  respectively. In this research, there were identified doubled haploid plants were developed from 4 F1 derived cross namely G2 (2 plants), G3 (4 plants),  G5 (21 plants), and G6 (26 plants).<br /><br />Keywords: Callus induction, doubled haploid, rice<br /><br />

scholarly journals Doubled haploids of interspecific hybrids between Brassica napus and Brassica rapa for canola production with valuable breeding traits

OCL ◽  
2020 ◽  
Vol 27 ◽  
pp. 45
Author(s):  
Ainash Daurova ◽  
Dias Daurov ◽  
Dmitriy Volkov ◽  
Kuanysh Zhapar ◽  
Daniyar Raimbek ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Seed Germination ◽  
Brassica Rapa ◽  
Doubled Haploid ◽  
Interspecific Hybrids ◽  
Nutritional Value ◽  
Doubled Haploids ◽  
Doubled Haploid Plants ◽  
Haploid Plants

Doubled haploids (DH) were obtained from two interspecific hybrids between Brassica napus and Brassica rapa. Seeds of doubled haploid plants differed in colour and size. The hybridity of the obtained doubled haploid is shown using genomic in situ hybridization (GISH) analysis. Evaluation of drought tolerance during seed germination on PEG-6000 showed the advantage of doubled haploid plants of interspecific hybrids over the parent cultivars. The oil from seeds of doubled haploid plants showed good nutritional value.

Production of doubled-haploid plants from Lilium longiflorum Thunb. anther culture

Plant Science ◽  
1997 ◽  
Vol 123 (1-2) ◽  
pp. 179-187 ◽  
Author(s):  
Amaury-M. Arzate-Fernández ◽  
Tetsuya Nakazaki ◽  
Hirotada Yamagata ◽  
Takatoshi Tanisaka
Keyword(s):  
Anther Culture ◽  
Doubled Haploid ◽  
Lilium Longiflorum ◽  
Doubled Haploid Plants ◽  
Haploid Plants

Anther-derived haploids of the African violet

1975 ◽  
Vol 53 (14) ◽  
pp. 1442-1444 ◽  
Author(s):  
Karen W. Hughes ◽  
Sandra L. Bell ◽  
James D. Caponetti
Keyword(s):  
Anther Culture ◽  
Root Formation ◽  
Indoleacetic Acid ◽  
Low Frequency ◽  
African Violet ◽  
Saintpaulia Ionantha ◽  
Haploid Plants

Anther culture of the African violet, Saintpaulia ionantha (Gesneriaceae), produces haploid plants directly from anthers at a low frequency. Successful anther culture for either this species or family has not been previously reported. Anthers between 3 mm and 5 mm in diameter are most suitable for culture. Root formation must be induced by the addition of indoleacetic acid to the medium.

scholarly journals Gametic Embryogenic Response in Wild Diploid Solanum Species and its Implications for Genome Sequencing Projects and Breeding

2016 ◽  
Vol 26 (2) ◽  
pp. 159-173
Author(s):  
A Castillo ◽  
P Gaiero ◽  
B López Carro ◽  
F Vilaró
Keyword(s):  
Anther Culture ◽  
Culture Media ◽  
Solanum Species ◽  
Chromosome Counts ◽  
Solanum Commersonii ◽  
Potato Germplasm ◽  
Anther Culture Response ◽  
Anther Cultures ◽  
Embryogenic Response ◽  
Haploid Plants

The anther culture response in Solanum commersonii (2n = 2x = 24, 1EBN) and S. chacoense (2n = 2x = 24, 2EBN), two wild potato germplasm resources was studied to obtain haploid plants. Three accessions from each of the two species and 3200 anthers from each genotype were cultured. Authors assessed different culture media; ascorbic acid, L?cysteine and silver nitrate (AgNO3) were included to prevent browning of anther cultures. Addition of AgNO3, was effective to induce embryogenesis. The clones from S. commersonii showed different embryogenic response to androgenesis. However, the three accessions from S. chacoense did not induce any embryo in the same conditions. Ploidy level of the regenerated clones was estimated by flow cytometry and confirmed by chromosome counts. This is the first report of haploid plants obtained from anther culture in S. commersonii, with important implications in sequencing efforts and potato breeding.Plant Tissue Cult. & Biotech. 26(2): 159-173, 2016 (December)

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