Cyclic nucleotides vs. adenosine analogs as inhibitors of adenylate cyclase activity: Nonidentity of sites of action

1975 ◽  
Vol 31 (8) ◽  
pp. 892-894 ◽  
Author(s):  
I. Weinryb ◽  
I. M. Michel
Keyword(s):  
Adenylate Cyclase ◽  
Cyclic Nucleotides ◽  
Cyclase Activity ◽  
Adenylate Cyclase Activity ◽  
Adenosine Analogs ◽  
Sites Of Action

Fluid secretion by isolated Malpighian tubules of Drosophila melanogaster Meig.: effects of organic anions, quinacrine and a diuretic factor found in the secreted fluid

1999 ◽  
Vol 202 (17) ◽  
pp. 2339-2348 ◽  
Author(s):  
J.A. Riegel ◽  
R.W. Farndale ◽  
S.H. Maddrell
Keyword(s):  
Drosophila Melanogaster ◽  
Adenylate Cyclase ◽  
Cyclic Nucleotides ◽  
Fluid Secretion ◽  
Organic Anions ◽  
Malpighian Tubules ◽  
Cyclase Activity ◽  
Adenylate Cyclase Activity ◽  
Phenol Red ◽  
Stimulation Of

Para-aminohippuric acid (PAH, 0.2 and 1 mmol l(−)(1)) had no effect on the basal fluid secretion rate (FSR) of isolated Malpighian tubules of Drosophila melanogaster Meig. and did not affect stimulation of the FSR induced by adenosine 3′,5′-monophosphate (cAMP). Phenol Red (phenolsulphonphthalein, PSP; 0.5 and 1 mmol l(−)(1)) slowed the FSR and abolished stimulation of the FSR by cAMP. Diodrast (1 mmol l(−)(1)) slightly, but significantly, reduced the FSR and greatly reduced the stimulation of the FSR normally provoked by cAMP and by the 3′,5′-monophosphates of guanosine (cGMP), inosine (cIMP) and uridine (cUMP). However, stimulation of the FSR by the 3′, 5′-monophosphate of cytidine (cCMP) was little affected by diodrast. Probenecid (0.2 or 1 mmol l(−)(1)) consistently stimulated the FSR, on average by approximately 25 %, but did not markedly inhibit the subsequent stimulation of the FSR by cAMP, cGMP or cIMP. However, the FSR of tubules stimulated by cGMP was temporarily lowered by probenecid. Quinacrine (0.1 mmol l(−)(1)) slowed basal FSR by an average of approximately 30 %, but subsequent stimulation of the FSR by cAMP was not noticeably affected. Both 0.1 mmol l(−)(1) cAMP and 1 mmol l(−)(1) probenecid stimulated adenylate cyclase activity in extracts of Malpighian tubules, but cIMP, cGMP, cUMP and diodrast were without effect in this regard. Uptake of radioactivity from a solution containing 500 nmol l(−)(1) [(3)H]cAMP and 9.5 μmol l(−)(1) cAMP was reduced by more than 90 % by 1 mmol l(−)(1) PSP, by approximately 40 % by 0.2 mmol l(−)(1) probenecid, by 36 % by 1 mmol l(−)(1) diodrast and by 30 % by 1 mmol l(−)(1) PAH. Neither 0.01 mmol l(−)(1) ouabain nor 0.1 mmol l(−)(1) quinacrine affected the uptake of [(3)H]cAMP by the Malpighian tubules. Fluid secreted by isolated Malpighian tubules of Drosophila melanogaster contains a factor that stimulated the FSR on average by approximately 50 %. The presence in the secreted fluid of cGMP at a concentration of 8.3 μmol l(−)(1) did not explain the stimulatory effect on FSR. These results support the existence of a carrier-mediated uptake of cyclic nucleotides into the Malpighian tubules of Drosophila melanogaster, possibly involving a multispecific transporter.

Cytochemical Evidence for Presynatic β-Adrenergic Regulation of Secretion in the Developing Rat Submandibular Gland

1977 ◽  
Vol 35 ◽  
pp. 430-431
Author(s):  
L.S. Cutler
Keyword(s):  
Cyclic Amp ◽  
Adenylate Cyclase ◽  
Submandibular Gland ◽  
Neonatal Rat ◽  
Cyclase Activity ◽  
Adenylate Cyclase Activity ◽  
Parotid Glands ◽  
Adrenergic Agonist ◽  
Rat Submandibular Gland

Many studies previously have shown that the B-adrenergic agonist isoproterenol and the a-adrenergic agonist norepinephrine will stimulate secretion by the adult rat submandibular (SMG) and parotid glands. Recent data from several laboratories indicates that adrenergic agonists bind to specific receptors on the secretory cell surface and stimulate membrane associated adenylate cyclase activity which generates cyclic AMP. The production of cyclic AMP apparently initiates a cascade of events which culminates in exocytosis. During recent studies in our laboratory it was observed that the adenylate cyclase activity in plasma membrane fractions derived from the prenatal and early neonatal rat submandibular gland was retractile to stimulation by isoproterenol but was stimulated by norepinephrine. In addition, in vitro secretion studies indicated that these prenatal and neonatal glands would not secrete peroxidase in response to isoproterenol but would secrete in response to norepinephrine. In contrast to these in vitro observations, it has been shown that the injection of isoproterenol into the living newborn rat results in secretion of peroxidase by the SMG (1).

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