An immunohistochemical employer monoclonal antibodies against Lea, sialyl Lea, Lex, and sialyl Lex antigens in primary colorectal, carcinomas and lymph node and hepatic lesions

1994 ◽  
Vol 29 (2) ◽  
pp. 129-138 ◽  
Author(s):  
Tohru Nakagoe ◽  
Kiyoyasu Fukushima ◽  
Masaki Hirota ◽  
Hiroyuki Kusano ◽  
Hiroyoshi Ayabe ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Lymph Node ◽  
Colorectal Carcinomas ◽  
Sialyl Lex ◽  
Hepatic Lesions ◽  
Sialyl Lea

scholarly journals Screening and development of monoclonal antibodies for identification of ferret T follicular helper cells

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Wenbo Jiang ◽  
Julius Wong ◽  
Hyon-Xhi Tan ◽  
Hannah G. Kelly ◽  
Paul G. Whitney ◽  
...  
Keyword(s):  
Animal Model ◽  
Monoclonal Antibodies ◽  
Lymph Node ◽  
Cross Reactivity ◽  
Tfh Cells ◽  
Follicular Helper ◽  
Lymph Node Cells ◽  
Human Viruses ◽  
Humoral Responses ◽  
Murine Monoclonal Antibodies

AbstractThe ferret is a key animal model for investigating the pathogenicity and transmissibility of important human viruses, and for the pre‐clinical assessment of vaccines. However, relatively little is known about the ferret immune system, due in part to a paucity of ferret‐reactive reagents. In particular, T follicular helper (Tfh) cells are critical in the generation of effective humoral responses in humans, mice and other animal models but to date it has not been possible to identify Tfh in ferrets. Here, we describe the screening and development of ferret-reactive BCL6, CXCR5 and PD-1 monoclonal antibodies. We found two commercial anti-BCL6 antibodies (clone K112-91 and clone IG191E/A8) had cross-reactivity with lymph node cells from influenza-infected ferrets. We next developed two murine monoclonal antibodies against ferret CXCR5 (clone feX5-C05) and PD-1 (clone fePD-CL1) using a single B cell PCR-based method. We were able to clearly identify Tfh cells in lymph nodes from influenza infected ferrets using these antibodies. The development of ferret Tfh marker antibodies and the identification of ferret Tfh cells will assist the evaluation of vaccine-induced Tfh responses in the ferret model and the design of novel vaccines against the infection of influenza and other viruses, including SARS-CoV2.

Immunoexpression of metalloproteinase 14 and tissue inhibitor of metalloproteinase 2 in colorectal carcinomas and lymph node metastases

2015 ◽  
Vol 24 (6) ◽  
pp. 1367-1376
Author(s):  
Francisco Nélson Nóbrega Furtado ◽  
João Paulo Aguiar Sampaio ◽  
João Tarcisio Alves Maia-Filho ◽  
Renato Braga Vieira ◽  
Roberto César Pereira Lima-Júnior ◽  
...  
Keyword(s):  
Lymph Node ◽  
Lymph Node Metastases ◽  
Tissue Inhibitor Of Metalloproteinase ◽  
Colorectal Carcinomas ◽  
Tissue Inhibitor ◽  
Node Metastases

Fucosyltransferase-producing sialyl Lea and sialyl Lex carbohydrate antigen in benign and malignant gastrointestinal mucosa

Cancer ◽  
1994 ◽  
Vol 73 (6) ◽  
pp. 1552-1561 ◽  
Author(s):  
Taeko Dohi ◽  
Mitsuko Hashiguchi ◽  
Shigeru Yamamoto ◽  
Hiroyoshi Morita ◽  
Mieko Oshima
Keyword(s):  
Carbohydrate Antigen ◽  
Gastrointestinal Mucosa ◽  
Sialyl Lex ◽  
Sialyl Lea

Genetic dissimilarity between primary colorectal carcinomas and their lymph node metastases: ploidy, p53, bcl-2, and c-myc expression—a pilot study

Tumor Biology ◽  
2015 ◽  
Vol 36 (8) ◽  
pp. 6579-6584 ◽  
Author(s):  
Khaled Refaat Zalata ◽  
Mohamed Farouk Elshal ◽  
Abd AlRahman Mohammad Foda ◽  
Ashraf Shoma
Keyword(s):  
Lymph Node ◽  
Pilot Study ◽  
Lymph Node Metastases ◽  
Colorectal Carcinomas ◽  
Node Metastases ◽  
Genetic Dissimilarity

Comparison of L-selectin and E-selectin ligand specificities: The L-selectin can bind the E-selectin ligands Sialyl Lex and Sialyl Lea

1992 ◽  
Vol 184 (2) ◽  
pp. 1048-1055 ◽  
Author(s):  
Ellen L. Berg ◽  
John Magnani ◽  
R.Aaron Warnock ◽  
Martyn K. Robinson ◽  
Eugene C. Butcher
Keyword(s):  
Selectin Ligands ◽  
Sialyl Lex ◽  
Selectin Ligand ◽  
Sialyl Lea
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