Environmental factors influencing aphid transmission of potato virus Y and potato leafroll virus

1988 ◽  
Vol 31 (3) ◽  
pp. 501-509 ◽  
Author(s):  
M. N. Singh ◽  
S. M. Paul Khurana ◽  
B. B. Nagaich ◽  
H. O. Agrawal
Keyword(s):  
Environmental Factors ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Aphid Transmission ◽  
Potato Leafroll Virus ◽  
Factors Influencing ◽  
Leafroll Virus

scholarly journals Factors influencing detection of Potato Leafroll Virus and Potato Virus Y in potato tuber extracts

2009 ◽  
Vol 59 (1) ◽  
pp. 65-74 ◽  
Author(s):  
Krzysztof Treder ◽  
Włodzimierz Przewodowski ◽  
Agnieszka Barnyk
Keyword(s):  
Potato Tuber ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Enzymatic Reaction ◽  
Potato Leafroll Virus ◽  
Dormancy Breaking ◽  
Signal Ratio ◽  
Factors Influencing ◽  
Leafroll Virus ◽  
Das Elisa

Factors influencing detection of Potato Leafroll Virus and Potato Virus Y in potato tuber extracts Detection of Potato leafroll virus (PLRV) and Potato virus Y (PVY) directly in potato tubers has been influenced by several factors. The most important were: the place of tuber sampling, preincubation of tuber sap before loading into wells of microplate and duration of tubers storage after collecting from field. The concentration of both viruses was highest in the heel part of tubers, whenever tested. Preincubation of tuber sap for several hours improved true/false signal ratio for dormant tubers and enabled reliable detection of both viruses. However after natural dormancy breaking it was necessary to change Cocktail-ELISA procedure to obtain reliable results, consistent with DAS-ELISA on leaves. The sap was not preincubated but loaded into wells directly after sample collecting and immuno-enzymatic reaction was developed overnight in refrigerator.

scholarly journals Resistência de genótipos de batata ao vírus do enrolamento da folha da batata (PLRV) e ao vírus Y (PVY)

2004 ◽  
Vol 22 (3) ◽  
pp. 521-524 ◽  
Author(s):  
Julio Daniels ◽  
Arione da S. Pereira
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Rio Grande ◽  
Potato Leafroll Virus ◽  
Rio Grande Do Sul ◽  
Leafroll Virus ◽  
Das Elisa

O vírus do enrolamento da folha da batata (Potato leafroll virus, PLRV) e o vírus Y da batata (Potato virus Y, PVY) constituem as principais causas da degenerescência da batata-semente no Brasil. Com o objetivo de determinar, nas condições do Rio Grande do Sul, a resistência de campo de genótipos de batata à infecção por estes vírus, avaliaram-se, na presença de infectores, durante três plantios consecutivos de primavera, 20 cultivares e clones de batata. A detecção dos vírus foi efetuada por meio de testes sorológicos (DAS-ELISA). Pela análise de agrupamento os genótipos foram separados em três grupos para resistência ao PLRV (Elvira, Achat, Bintje, Monalisa, Monte Bonito, Panda e Araucária, resistentes; Baronesa, Asterix, Atlantic, 2CRI-1149-1-78, C-1226-35-80, Astrid, C-1714-7-94, A-1139-12-92, Macaca, Eliza e Santo Amor, suscetíveis; Catucha e Cristal, muito suscetíveis) e em quatro grupos para resistência ao PVY (Asterix, Astrid, Catucha, Cristal, Macaca, Monte Bonito, A-1139-12-92, C-1226-35-80 e C-1714-7-94, resistentes; Baronesa, Santo Amor, Monalisa, Panda e 2CRI-1149-1-78, resistentes intermediários; Bintje, Atlantic, Elvira e Araucária, suscetíveis; Achat e Eliza, muito suscetíveis).

scholarly journals Multiple DNA markers for evaluation of resistance against Potato virus Y, Potato virus S and Potato leafroll virus

2018 ◽  
Vol 54 (No. 1) ◽  
pp. 30-33 ◽  
Author(s):  
M. Naderpour ◽  
L. Sadeghi
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Simultaneous Detection ◽  
Potato Leafroll Virus ◽  
Potato Seed ◽  
Breeding Programs ◽  
Potato Virus S ◽  
Sequence Characterized Amplified Region ◽  
Leafroll Virus ◽  
Resistance To Viruses

Molecular markers within or close to genes of interest play essential roles in marker-assisted selection. PCR-based markers have been developed for numerous traits in different plant species including several genes conferring resistance to viruses in potato. In the present work, rapid and reliable approaches were developed for the simultaneous detection of Ryadg and Ry-fsto, Ns, and PLRV.1 genes conferring resistance to Potato virus Y, Potato virus S and Potato leafroll virus, respectively, on the basis of previously published and newly modified markers. The sequence characterized amplified region (SCAR) markers for Ryadg, Ns and PLRV1 and the newly modified cleaved amplified polymorphic sequences (CAPS) marker for Ry-fsto were amplified in one PCR reaction which could simply characterize Ryadg and PLRV.1 resistance. Additional digestion of amplicons with EcoRV and MfeI for genotyping the Ry-fsto and Ns resistance genes, respectively, was needed. The effectiveness of genotyping in triplex and tetraplex PCRs was tested on 35 potato varieties used for potato seed production and breeding programs.  

scholarly journals The Effects of High Temperature on Infection by Potato virus Y, Potato virus A, and Potato leafroll virus

2016 ◽  
Vol 32 (4) ◽  
pp. 321-328 ◽  
Author(s):  
Bong Nam Chung ◽  
Tomas Canto ◽  
Francisco Tenllado ◽  
Kyung San Choi ◽  
Jae Ho Joa ◽  
...  
Keyword(s):  
High Temperature ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Potato Leafroll Virus ◽  
Potato Virus A ◽  
Leafroll Virus

scholarly journals Detection and sequencing of Potato virus Y (PVY) and Potato leafroll virus (PLRV) in a volunteer plant of Solanum tuberosum L. cv. Diacol-Capiro

2017 ◽  
Vol 66 (4) ◽  
pp. 625-632 ◽  
Author(s):  
Héctor Camilo Medina Cárdenas ◽  
Pablo Andrés Gutiérrez Sánchez ◽  
Mauricio Alejandro Marín Montoya
Keyword(s):  
Solanum Tuberosum ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Solanum Tuberosum L ◽  
Potato Leafroll Virus ◽  
Leafroll Virus ◽  
Volunteer Plant
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