Perspective on hepatic peroxisomes and pancreatic hepatocytes

1994 ◽  
Vol 27 (3-4) ◽  
pp. 181-190
Author(s):  
Janardan K. Reddy ◽  
Keith Alvares
Keyword(s):  
Pancreatic Hepatocytes

Pancreatic hepatocytes in hamsters (Mesocricetus auratus) infected with Trypanosoma cruzi

2002 ◽  
Vol 100 (2) ◽  
pp. 103-111 ◽  
Author(s):  
Vitorino Modesto dos Santos ◽  
Marcus Aurelho de Lima ◽  
Marlene Cabrine-Santos ◽  
Daniela de Stefani Marquez ◽  
Maria das Graças Reis ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Mesocricetus Auratus ◽  
Pancreatic Hepatocytes

scholarly journals Androgen regulated expression of the alpha 2u-globulin gene in pancreatic hepatocytes of rat.

1990 ◽  
Vol 110 (2) ◽  
pp. 263-267 ◽  
Author(s):  
R S Dwivedi ◽  
A V Yeldandi ◽  
V Subbarao ◽  
P Feigelson ◽  
A K Roy ◽  
...  
Keyword(s):  
Female Rats ◽  
Male Rats ◽  
Immunoperoxidase Method ◽  
Adult Rat ◽  
Male And Female Rats ◽  
Regulated Expression ◽  
Androgenic Regulation ◽  
Normal Males ◽  
Parenchymal Cells ◽  
Pancreatic Hepatocytes

Under a copper-deficient regimen, pancreatic cells in the adult rat can be found to undergo differentiation into hepatocytes. Pancreatic hepatocytes induced in male and female rats were examined for the expression of the androgen-inducible hepatic protein, alpha 2u-globulin. Alpha 2u-Globulin protein was demonstrable by immunoperoxidase method in all the pancreatic hepatocytes of male rats. Northern blot analysis confirmed the presence of 1.3 kb alpha 2u-globulin mRNA transcript in the pancreas of male rats with hepatocytes. Orchiectomy resulted in marked decrease of alpha 2u-globulin protein and its mRNA. Administration of dihydrotestosterone to castrated rats resulted in increased levels of alpha 2u-globulin mRNA and the amount of alpha 2u-globulin protein in the pancreatic hepatocytes. Unlike normal males, in intact and ovariectomized females alpha 2u-globulin was not detectable in pancreatic hepatocytes. These results indicate that similar to hepatic parenchymal cells pancreatic hepatocytes synthesize alpha 2u-globulin under androgenic regulation. Furthermore, unlike in liver where it is expressed predominantly in perivenular and midlobular hepatocytes, there is no localized difference in the expression of this gene in the transdifferentiated pancreatic hepatocytes.

Immunohistochemical Evidence of High Concentrations of Metallothionein in Pancreatic Hepatocytes Induced by Cadmium in Rats

1992 ◽  
Vol 20 (3-1) ◽  
pp. 323-326 ◽  
Author(s):  
Michael P. Waalkes ◽  
M. George Cherian ◽  
Jerrold M. Ward ◽  
Robert A. Goyer
Keyword(s):  
High Concentrations ◽  
Immunohistochemical Evidence ◽  
Pancreatic Hepatocytes

Pancreatic Hepatocytes Associated with Chronic 2,6-Dichloro-p-Phenylenediamine Administration in Fischer 344 Rats

1989 ◽  
Vol 17 (1_part_1) ◽  
pp. 1-6 ◽  
Author(s):  
Margarita M. McDonald ◽  
Gary A. Boorman
Keyword(s):  
Light Microscopy ◽  
Pancreatic Tissue ◽  
Female Rats ◽  
Fischer 344 Rats ◽  
Fischer 344 ◽  
Relationship Of ◽  
The Relationship ◽  
Pancreatic Hepatocytes

Pancreatic tissue (original and recut sections) from Fischer 344 rats fed 2,6-dichloro-p-phenylenediamine in a chronic (2-year) carcinogenesis bioassay was evaluated for presence of pancreatic hepatocytes (PH) by light microscopy. PH were found in dose groups as follows: males–0 ppm (controls)–0/50 (0%), 1,000 ppm–4/50 (8%), 2,000 ppm–9/50 (18%); females-0 ppm (controls)–1/50 (2%), 2,000 ppm–15/50 (30%), 6,000 ppm–15/49 (31%). This represented a significant dose-related increased incidence of PH in 2,000-ppm males, and 2,000- and 6,000-ppm females. A statistically significant increase ( p < 0.01) in pancreatic acinar atrophy and fibrosis was also seen in treated female rats, but the relationship of these lesions to the PH is unclear.

scholarly journals Pancreatic hepatocytes in B6C3F1 (C57BL/6CrSlc*C3H/HeSlc) and BDF1 (C57BL/6NCrj*DBA/2NCrj) mice.

1995 ◽  
Vol 8 (2) ◽  
pp. 117-121 ◽  
Author(s):  
Seiki Yamakawa ◽  
Hijiri Iwata ◽  
Yasuhiko Hirouchi ◽  
Kazuo Kobayashi ◽  
Makoto Enomoto
Keyword(s):  
Pancreatic Hepatocytes

scholarly journals A New Model for the Investigation of Pancreatic Hepatocytes.

1991 ◽  
Vol 53 (5) ◽  
pp. 937-939
Author(s):  
Yoshinori SUGAWARA ◽  
Shin-ichi ITAGAKI ◽  
Kunio DOI
Keyword(s):  
New Model ◽  
Pancreatic Hepatocytes

scholarly journals Coexpression of glutamine synthetase and carbamoylphosphate synthase I genes in pancreatic hepatocytes of rat.

1990 ◽  
Vol 87 (3) ◽  
pp. 881-885 ◽  
Author(s):  
A. V. Yeldandi ◽  
X. D. Tan ◽  
R. S. Dwivedi ◽  
V. Subbarao ◽  
D. D. Smith ◽  
...  
Keyword(s):  
Glutamine Synthetase ◽  
Pancreatic Hepatocytes

Pancreatic hepatocytes

1991 ◽  
Vol 36 (4) ◽  
pp. 502-509 ◽  
Author(s):  
Janardan K. Reddy ◽  
M. Sambasiva Rao ◽  
Anjana V. Yeldandi ◽  
Xiaodi Tan ◽  
Rama S. Dwivedi
Keyword(s):  
Pancreatic Hepatocytes

Expression and compartmentalization of integral plasma membrane proteins by hepatocytes and their progenitors in the rat pancreas

1991 ◽  
Vol 98 (1) ◽  
pp. 45-54
Author(s):  
J.R. Bartles ◽  
M.S. Rao ◽  
L.Q. Zhang ◽  
B.E. Fayos ◽  
C.L. Nehme ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Membrane Proteins ◽  
Membrane Protein ◽  
Apical Plasma Membrane ◽  
Specific Marker ◽  
Plasma Membrane Protein ◽  
Plasma Membrane Proteins ◽  
Dipeptidylpeptidase Iv ◽  
Copper Depletion ◽  
Pancreatic Hepatocytes

A combination of Western blotting, Northern blotting and immunofluorescence was used to examine the expression and compartmentalization of plasma membrane proteins by those hepatocyte-like cells that arise in the pancreases of rats subjected to sequential dietary copper depletion and repletion. The pancreatic hepatocytes were found to: (1) express several integral membrane proteins known to be concentrated within the apical, lateral or basolateral domains of the plasma membranes of hepatocytes in liver; and (2) compartmentalize the membrane proteins to equivalent plasma membrane domains, despite the organization of these cells into clusters instead of highly vascularized plates. The apical plasma membrane proteins dipeptidylpeptidase IV and HA 4 were found to line bile canaliculus-like openings between adjacent pancreatic hepatocytes; these openings were shown to be continuous with the pancreatic exocrine duct by India ink infusion. In contrast, the basolateral plasma membrane protein rat hepatic lectin-1 and lateral plasma membrane protein HA 321 were detected elsewhere about the surfaces of the pancreatic hepatocytes: by analogy to their respective localizations on hepatocytes in liver, rat hepatic lectin-1 was concentrated on those surfaces exposed to the pancreatic matrix at the periphery of the hepatocyte clusters (the basal surface equivalent), whereas HA 321 was concentrated on those surfaces exposed to adjacent hepatocytes within the clusters. The hepatocyte plasma membrane proteins were found to be expressed in the pancreas at different times during the copper depletion/repletion protocol: for example, rat hepatic lectin-1 and the bulk of the HA 4 were expressed relatively late in the protocol, only after large numbers of pancreatic hepatocytes had appeared; whereas dipeptidylpeptidase IV was induced greater than 10-fold early in the protocol and proved to be an apical-specific marker for those ductular epithelial cells that are believed to be the progenitors of the pancreatic hepatocytes.

scholarly journals Induction and origin of hepatocytes in rat pancreas.

1984 ◽  
Vol 98 (6) ◽  
pp. 2082-2090 ◽  
Author(s):  
J K Reddy ◽  
M S Rao ◽  
S A Qureshi ◽  
M K Reddy ◽  
D G Scarpelli ◽  
...  
Keyword(s):  
Endocrine Cells ◽  
Secretory Granules ◽  
Volume Density ◽  
Peroxisome Proliferator ◽  
Rat Hepatocyte ◽  
Transitional State ◽  
Transitional Cells ◽  
Acinar Tissue ◽  
Intermediate Cells ◽  
Pancreatic Hepatocytes

2-[4(2,2- Dichlorocyclopropyl )phenoxy]2-methyl propionic acid (ciprofibrate), a peroxisome proliferator , induced hepatocytes in the pancreas of adult male F-344 rats when added to their diet at a dosage of 10 mg/kg body weight for 60-72 wk. These cells are morphologically indistinguishable from hepatic hepatocytes and were usually localized adjacent to islets of Langerhans with extensions into surrounding acinar tissue. A significant increase in the volume density of peroxisomes, together with immunochemically detectable amounts of two peroxisome-associated enzymes, was observed in pancreas with hepatocytes of rats maintained on ciprofibrate. Uricase-containing crystalloid nucleoids, specific for rat hepatocyte peroxisomes, were present in pancreatic hepatocytes. These structures facilitated the identification of cells with hybrid cytoplasmic features characteristic of pancreatic acinar and endocrine cells and hepatocytes. Such cells are presumed to represent a transitional state in which pancreas specific genes are being repressed while liver specific ones are simultaneously expressed. The presence of exocrine and/or endocrine secretory granules in transitional cells indicates that acinar/intermediate cells represent the precursor cell from which pancreatic hepatocytes are derived.

Sign in / Sign up

Export Citation Format

Share Document