Lipid composition and fatty acid analysis ofHelicobacter pylori

1995 ◽  
Vol 30 (3) ◽  
pp. 315-318 ◽  
Author(s):  
Yoshihito Inamoto ◽  
Sumiko Hamanaka ◽  
Yuichiro Hamanaka ◽  
Takatoshi Nagate ◽  
Isamu Kondo ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Lipid Composition ◽  
Fatty Acid Analysis

scholarly journals Dissecting lipid contents in the distinct regions of native retinal rod disk membranes

2021 ◽  
Author(s):  
Christopher L. Sander ◽  
Avery E. Sears ◽  
Antonio F. M. Pinto ◽  
Elliot H. Choi ◽  
Shirin Kahremany ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Membrane Proteins ◽  
Lipid Composition ◽  
Protein Localization ◽  
Specific Protein ◽  
Fatty Acid Analysis ◽  
Protein Activity ◽  
Domain Specific ◽  
Retinal Rod ◽  
Membrane Compartments

ABSTRACTPhotoreceptors rely on distinct membrane compartments to support their specialized function. Unlike protein localization, identification of critical differences in membrane content has not yet been expanded to lipids, due to the difficulty of isolating domain-specific samples. We have overcome this by using SMA to co-immunopurify membrane proteins and their native lipids from two regions of photoreceptor ROS disks. Each sample’s copurified lipids were subjected to untargeted lipidomic and fatty acid analysis. Extensive differences between center (rhodopsin) and rim (ABCA4 and PRPH2/ROM1) samples included a lower PC to PE ratio and increased LC- and VLC-PUFAs in the center relative to the rim region, which were enriched in shorter, saturated FAs. The comparatively few differences between the two rim samples likely reflect specific protein-lipid interactions. High-resolution profiling of the ROS disk lipid composition provides a model for future studies of other complex cellular structures, and gives new insights into how intricate membrane structure and protein activity are balanced within the ROS.SUMMARYSander et al. have parsed the lipid composition of native-source photoreceptor disks and find large differences in fatty acid unsaturation and chain length between the center and rim regions. They selectively copurify membrane proteins and lipids from each region in SMALPs using nanobodies and antibodies.

Chemical Constituents in Some Promising Genotypes of Indian Mustard [Brassica juncea (L.) Czern and Coss.]

2017 ◽  
Vol 4 (04) ◽  
Author(s):  
SUNITA SINGH ◽  
R. P. SINGH ◽  
H. K. SINGH ◽  
N. A. KHAN ◽  
M. K. MAURYA
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Erucic Acid ◽  
Brassica Juncea ◽  
Acid Composition ◽  
Chemical Constituents ◽  
Indian Mustard ◽  
Acid Value ◽  
Fatty Acid Analysis ◽  
Crude Fibre

Among the oilseed Brassica crops, Indian mustard [Brassica juncea (L.) Czern and Coss.] is an important source of oil from a nutritional point of view. The nutritional value of oil and cake quality is governed mainly by the composition of its fatty acids, iodine value, saponification, acid value, glucosinolates, crude fibre, protein and limiting amino acids, etc. Seventeen varieties/strains of Indian mustard were taken for saturated and unsaturated fatty acid analysis. The eicosenoic was absent in genotype (NUDBYJ-10) and erucic acid (NUDBYJ-10, LES-46 and Pusa mustard- 21). The fatty acid composition found a variable in different genotypes. Saturated fatty acid, Palmitic + Stearic ranged between 2.3 to 6.5%, Oleic 10.6 to 40.7%, Linoleic 16.1 to 37.7%, Linolenic 13.3 to 26.7%, Eicosenoic 0.00 to 10.30% and Erucic acid 0.00 to 47.50%, respectively. Alternaria blight severity also varied in different genotypes and ranged between 18.75 to 56.25%, maximum being in genotype Kranti and minimum in LES-47. No significant correlation was observed between the fatty acid composition and disease severity. The oil content range from 38.1 to 42.60% and protein content was found highest in variety RGN-73. The amino acid viz. methionine and tryptophan range between 0.41 to 1.81 g/16gN and 0.41 to 1.81 g /16g N, respectively.

EVLUETION OF DIETARY UTILIZATION OF BLOOM-FORMING CYANOBACTERIA BY SMALL-BODIED CRUSTACEAN ZOOPLANKTON USING FATTY ACID ANALYSIS

2019 ◽  
Vol 75 (7) ◽  
pp. III_367-III_374
Author(s):  
Souta AOMORI ◽  
Megumu FUJIABAYASHI ◽  
Kunihiro OKANO ◽  
Yoshihiro TAKADA ◽  
Naoyuki MIYATA
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Analysis ◽  
Crustacean Zooplankton
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