Streptomyces rimosus extracellular proteases 3. Isolation and characterization of leucine aminopeptidase

1986 ◽  
Vol 23 (6) ◽  
pp. 449-455 ◽  
Author(s):  
Lj. Vitale ◽  
M. Renko ◽  
B. Lenarčič ◽  
V. Turk ◽  
M. Pokorny
Keyword(s):  
Leucine Aminopeptidase ◽  
Streptomyces Rimosus ◽  
Extracellular Proteases ◽  
Isolation And Characterization

scholarly journals Streptomyces flavogriseusHS1: Isolation and Characterization of Extracellular Proteases and Their Compatibility with Laundry Detergents

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Sofiane Ghorbel ◽  
Maher Kammoun ◽  
Hala Soltana ◽  
Moncef Nasri ◽  
Noomen Hmidet
Keyword(s):  
High Stability ◽  
Biochemical Characterization ◽  
Protease Production ◽  
Detergent Additive ◽  
Extracellular Proteases ◽  
Original Activity ◽  
Isolation And Characterization ◽  
Secreted Proteases ◽  
Industrial Level

The present study describes the isolation of a new protease producingStreptomycesstrain HS1 and the biochemical characterization of the secreted proteases. By sequencing of its noted 16S rDNA, HS1 strain was found to have a 100% identity withStreptomyces flavogriseus. The highest protease production was found using FermII media. In these conditions maximum protease production (99 U/mL) was obtained after 96 h incubation at 30°C and 150 rpm. HS1 strain produced at least five proteases as revealed by zymogram technique. The enzyme preparation exhibited activity over a broad range of pH (5–11) and temperature (25–70°C). Optimum activity was observed at a pH of 7.0 and a temperature of 50°C. Proteolytic activity was significantly unaffected by Ca2+and Mg2+. EDTA and PMSF highly decreased the original activity. The crude extracellular proteases showed high stability when used as a detergent additive. These properties offer an interesting potential for enzymatic hydrolysis at the industrial level.

Isolation and characterization of mutants of Aspergillus niger deficient in extracellular proteases

1992 ◽  
Vol 234 (2) ◽  
pp. 332-336 ◽  
Author(s):  
Ineke E. Mattern ◽  
Johannes M. van Noort ◽  
Paul van den Berg ◽  
David B. Archer ◽  
Ian N. Roberts ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Extracellular Proteases ◽  
Isolation And Characterization

scholarly journals Isolation and Characterization of the Neutral Leucine Aminopeptidase (LapN) of Tomato

2003 ◽  
Vol 132 (1) ◽  
pp. 243-255 ◽  
Author(s):  
Chao-Jung Tu ◽  
Sang-Youl Park ◽  
Linda L. Walling
Keyword(s):  
Leucine Aminopeptidase ◽  
Isolation And Characterization

The isolation and characterization of 18 equine microsatellite loci, TKY272–TKY289

2000 ◽  
Vol 31 (2) ◽  
pp. 149-149 ◽  
Author(s):  
T Tozaki ◽  
H Kakoi ◽  
S Mashima ◽  
K Hirota ◽  
T Hasegawa ◽  
...  
Keyword(s):  
Microsatellite Loci ◽  
Isolation And Characterization
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