Evolution of the larval cuticle proteins coded by the secondary sex chromosome pair:X2 andneo-Y ofDrosophila miranda: I. Comparison at the DNA sequence level

1996 ◽  
Vol 43 (4) ◽  
pp. 405-412
Author(s):  
Manfred Steinemann ◽  
Sigrid Steinemann ◽  
Wilhelm Pinsker
Keyword(s):  
Dna Sequence ◽  
Sex Chromosome ◽  
Larval Cuticle ◽  
Cuticle Proteins

Purification and cDNA cloning of evolutionally conserved larval cuticle proteins of the silkworm, Bombyx mori

1997 ◽  
Vol 27 (8-9) ◽  
pp. 701-709 ◽  
Author(s):  
Hiroshi Nakato ◽  
Mariko Takekoshi ◽  
Toru Togawa ◽  
Susumu Izumi ◽  
Shiro Tomino
Keyword(s):  
Bombyx Mori ◽  
Cdna Cloning ◽  
Larval Cuticle ◽  
Cuticle Proteins ◽  
Silkworm Bombyx Mori

Larval cuticle proteins of Lucilia cuprina

1987 ◽  
Vol 17 (4) ◽  
pp. 625-633 ◽  
Author(s):  
P.J. Skelly ◽  
A.J. Howells
Keyword(s):  
Lucilia Cuprina ◽  
Larval Cuticle ◽  
Cuticle Proteins

Pupal and larval cuticle proteins of Drosophila melanogaster

Biochemistry ◽  
1984 ◽  
Vol 23 (24) ◽  
pp. 5767-5774 ◽  
Author(s):  
Donald J. Silvert ◽  
John Doctor ◽  
Luis Quesada ◽  
James W. Fristrom
Keyword(s):  
Drosophila Melanogaster ◽  
Larval Cuticle ◽  
Cuticle Proteins

scholarly journals mRNA and Small RNA-seq Reveal Insights into Immune Regulation in Apis cerana after Chinese Sacbrood Virus Infection

2020 ◽  
Author(s):  
Yanchun Deng ◽  
Hongxia Zhao ◽  
Shuo Shen ◽  
Sa Yang ◽  
Dahe Yang ◽  
...  
Keyword(s):  
Immune Response ◽  
Fatty Acid ◽  
Target Genes ◽  
Fatty Acid Biosynthesis ◽  
Apis Cerana ◽  
Larval Cuticle ◽  
Go Annotation ◽  
Kegg Analysis ◽  
Sacbrood Virus ◽  
Cuticle Proteins

Chinese sacbrood virus (CSBV) is a serious threat to eastern honeybees (Apis cerana), especially larvae. However, the pathological mechanism of this deadly disease is remains unclear. Here, we employed an mRNA-seq and sRNA-seq approach in healthy and CSBV-infected 3rd Apis cerana larval. Gene ontology (GO) and KEGG analysis of 203 differentially expressed genes showed that CSBV infection affected host development by up-regulating the expression of larval cuticle proteins, such as larval cuticle proteins A1A and A3A, resulting in elevated susceptibility to CSBV. In addition, viral infection not only affected the expression of serine protease related to the melanization pathway and but also altered fatty acid metabolism and biosynthesis, thus progressed to disturb host immune response. Interestingly, GO annotation and KEGG analysis on target genes of CSBV-specific siRNA (vsiRNAs) showed that serine/threonine kinase activity and serine-type endopeptidas as well as fatty acid biosynthesis were significantly enriched (P < 0.05). Among these vsiRNAs, vsiRNA-1441 with relatively high expression targeted extracellular serine/threonine protein kinase. This study provides new evidence that CSBV attacks a distinct immune response pathway and mediates the expression of cuticle protein to gain the more chance of proliferation.

scholarly journals Isolation of a sex chromosome-specific DNA sequence in the medaka, Oryzias latipes.

1997 ◽  
Vol 72 (5) ◽  
pp. 263-268 ◽  
Author(s):  
Masaru Matsuda ◽  
Takehiko Kusama ◽  
Takashi Oshiro ◽  
Yasuyuki Kurihara ◽  
Satoshi Hamaguchi ◽  
...  
Keyword(s):  
Dna Sequence ◽  
Sex Chromosome ◽  
Oryzias Latipes

scholarly journals THE CUTICLE PROTEINS OF DROSOPHILA MELANOGASTER: GENETIC LOCALIZATION OF A SECOND CLUSTER OF THIRD-INSTAR GENES

Genetics ◽  
1986 ◽  
Vol 114 (2) ◽  
pp. 393-404
Author(s):  
Carol J Chihara ◽  
Deborah A Kimbrell
Keyword(s):  
Recessive Mutation ◽  
Larval Cuticle ◽  
Gene Map ◽  
The Third ◽  
Naturally Occurring ◽  
Induced Mutants ◽  
Recessive Mutant ◽  
Cuticle Protein ◽  
Cuticle Proteins ◽  
New Protein

ABSTRACT Five third-instar larval cuticle protein genes are placed on the left arm of the third chromosome. For these five genes, 12 variants and two induced mutants are described. All the naturally occurring variants are codominant. One EMS-induced mutant is characterized by the codominant appearance of a new protein, and a second by a recessive mutation that codes for a modifier of third-instar larval cuticle protein 5 (L3CP-5). All but the putative modifier gene map to within less than 0.3 map units of each other on the left arm of the third chromosome at approximately 11. We propose that these genes constitute a cluster of third-instar cuticle protein genes. The induced recessive mutant maps outside of the cluster to a region also on the left arm of the third chromosome. The proteins and their genes should prove useful for both developmental and evolutionary studies.

DNA sequence mapping in interphase and metaphase chromosomes by fluorescence in situ hybridization

1992 ◽  
Vol 50 (1) ◽  
pp. 496-497
Author(s):  
Barbara Trask ◽  
Susan Allen ◽  
Anne Bergmann ◽  
Mari Christensen ◽  
Anne Fertitta ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Dna Sequence ◽  
Dna Sequences ◽  
Dual Band ◽  
Nick Translation ◽  
Metaphase Chromosomes ◽  
Band Pass ◽  
Texas Red ◽  
Fluorescent Spot

Using fluorescence in situ hybridization (FISH), the positions of DNA sequences can be discretely marked with a fluorescent spot. The efficiency of marking DNA sequences of the size cloned in cosmids is 90-95%, and the fluorescent spots produced after FISH are ≈0.3 μm in diameter. Sites of two sequences can be distinguished using two-color FISH. Different reporter molecules, such as biotin or digoxigenin, are incorporated into DNA sequence probes by nick translation. These reporter molecules are labeled after hybridization with different fluorochromes, e.g., FITC and Texas Red. The development of dual band pass filters (Chromatechnology) allows these fluorochromes to be photographed simultaneously without registration shift.

Sign in / Sign up

Export Citation Format

Share Document