Primary structure of the alanine subunit of ricin T from the seeds of the Central Asian castor oil plant VI. Tryptic peptides of the cyanogen bromide fragments B-1, B-2, and B-3

1998 ◽  
Vol 34 (6) ◽  
pp. 715-719
Author(s):  
D. A. Khashimov ◽  
P. Kh. Yuldashev
Keyword(s):  
Primary Structure ◽  
Castor Oil ◽  
Cyanogen Bromide ◽  
Tryptic Peptides ◽  
Central Asian ◽  
Oil Plant

The primary structure of staphylococcal protease

1978 ◽  
Vol 56 (6) ◽  
pp. 534-544 ◽  
Author(s):  
Gabriel R. Drapeau
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Primary Structure ◽  
Serine Proteases ◽  
Cyanogen Bromide ◽  
Streptomyces Griseus ◽  
Tryptic Peptides ◽  
Single Polypeptide Chain ◽  
Sequence Homologies ◽  
Single Polypeptide

The amino acid sequence of staphylococcal protease has been determined by analysis of tryptic peptides obtained from cyanogen bromide fragments. Selected peptides obtained from digests with staphylococcal protease, thermolysin, and chymotrypsin provided the information necessary to align the tryptic peptides and the cyanogen bromide fragments. The protease is a single polypeptide chain of some 250 amino acids and is devoid of sulfhydryl groups. The COOH-terminal tryptic peptide of the protease molecule contains some 43 residues, most of which are aspartic acids, asparagines, and prolines. The amino acid sequence of this peptide was not determined. The primary structure near the active serine residue indicates that staphylococcal protease is related to the pancreatic serine proteases. However, it has little or no additional sequence homologies with these enzymes except for the regions near histidine-50 and aspartic acid - 91. These regions have striking similarities with the corresponding regions of protease B and the trypsin-like enzyme of Streptomyces griseus.

Primary structure of human intrinsic factor: progress report on cyanogen bromide fragmentation

1978 ◽  
Vol 38 (7) ◽  
pp. 649-653 ◽  
Author(s):  
Ebba Nexø ◽  
Henrik Olesen ◽  
Marianne Rye Hansen ◽  
Ditlef Bucher ◽  
Johannes Thomsen
Keyword(s):  
Primary Structure ◽  
Cyanogen Bromide ◽  
Intrinsic Factor ◽  
Progress Report
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