A simple molecular model for adsorption chromatography. IX. Comparison of adsorption mechanisms in thin-layer chromatography and high-speed liquid chromatography

1976 ◽  
Vol 9 (3) ◽  
pp. 113-118 ◽  
Author(s):  
W. Gołkiewicz
Keyword(s):  
Liquid Chromatography ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Speed ◽  
Molecular Model ◽  
Adsorption Chromatography ◽  
Adsorption Mechanisms ◽  
Layer Chromatography

Application of High-Speed Liquid Chromatography to the Analysis of Aflatoxins

1973 ◽  
Vol 56 (4) ◽  
pp. 827-830 ◽  
Author(s):  
James N Seiber ◽  
Dennis P H Hsieh
Keyword(s):  
Liquid Chromatography ◽  
Quantitative Analysis ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Porous Layer ◽  
High Speed ◽  
Aspergillus Parasiticus ◽  
Coefficients Of Variation ◽  
Silica Adsorbent ◽  
Layer Chromatography

Abstract Partial resolution of aflatoxins B1, B2, G1, G2, and P1 was achieved by high-speed liquid chromatography (HSLC) on a porous layer silica adsorbent, using chloroform-isooctane as the eluting solvent and a 254 nm UV monitor for detection. The resolution was somewhat less than, although comparable with, that obtained by thin layer chromatography, using Adsorbosil-1 adsorbent and fluorodensitometric detection. The HSLC response to B1 and G1 was linear in the 400–3000 ng range, allowing application of the technique to the quantitative analysis of B1 and G1 in crude extracts of Aspergillus parasiticus cultures. The coefficients of variation (precision) were 4.2% for B1 and 23.2% for G1 in a series of 4 replicate injections. The advantages and limitations of the technique for quantitative analysis and isolation are compared with those of more conventional chromatographic methods.

Phytochemical, Analytical and Medicinal Studies of Holoptelea integrifolia Roxb. Planch - A Review

2019 ◽  
Vol 5 (4) ◽  
pp. 270-277 ◽  
Author(s):  
Vijay Kumar ◽  
Simranjeet Singh ◽  
Ragini Bhadouria ◽  
Ravindra Singh ◽  
Om Prakash
Keyword(s):  
Liquid Chromatography ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Biologically Active ◽  
Toxicological Studies ◽  
Wide Range ◽  
Layer Chromatography

Holoptelea integrifolia Roxb. Planch (HI) has been used to treat various ailments including obesity, osteoarthritis, arthritis, inflammation, anemia, diabetes etc. To review the major phytochemicals and medicinal properties of HI, exhaustive bibliographic research was designed by means of various scientific search engines and databases. Only 12 phytochemicals have been reported including biologically active compounds like betulin, betulinic acid, epifriedlin, octacosanol, Friedlin, Holoptelin-A and Holoptelin-B. Analytical methods including the Thin Layer Chromatography (TLC), High-Performance Thin Layer Chromatography (HPTLC), High-Performance Liquid Chromatography (HPLC) and Liquid Chromatography With Mass Spectral (LC-MS) analysis have been used to analyze the HI. From medicinal potency point of view, these phytochemicals have a wide range of pharmacological activities such as antioxidant, antibacterial, anti-inflammatory, and anti-tumor. In the current review, it has been noticed that the mechanism of action of HI with biomolecules has not been fully explored. Pharmacology and toxicological studies are very few. This seems a huge literature gap to be fulfilled through the detailed in-vivo and in-vitro studies.

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