Staining yeast cells for electron microscopy by growth in copper-containing nutrient broth

1972 ◽  
Vol 38 (1) ◽  
pp. 17-26 ◽  
Author(s):  
C. C. Lindegren ◽  
Paraskevi M. BeMiller ◽  
Kuo -Chun Liu ◽  
Gertrude Lindegren
Keyword(s):  
Electron Microscopy ◽  
Nutrient Broth ◽  
Yeast Cells

Use of electron microscopy to characterize the surfaces of flocculent and nonflocculent yeast cells

1989 ◽  
Vol 35 (12) ◽  
pp. 1081-1086 ◽  
Author(s):  
Byron F. Johnson ◽  
L. C. Sowden ◽  
Teena Walker ◽  
Bong Y. Yoo ◽  
Gode B. Calleja
Keyword(s):  
Electron Microscopy ◽  
Fission Yeast ◽  
Budding Yeast ◽  
The Other ◽  
Yeast Cells ◽  
Scanning Electron Micrographs ◽  
Soft Or ◽  
Transmission Electron ◽  
Scanning Transmission ◽  
Scanning Electron

The surfaces of flocculent and nonflocculent yeast cells have been examined by electron microscopy. Nonextractive preparative procedures for scanning electron microscopy allow comparison in which sharp or softened images of surface details (scars, etc.) are the criteria for relative abundance of flocculum material. Asexually flocculent budding-yeast cells cannot be distinguished from nonflocculent budding-yeast cells in scanning electron micrographs because the scar details of both are well resolved, being hard and sharp. On the other hand, flocculent fission-yeast cells are readily distinguished from nonflocculent cells because fission scars are mostly soft or obscured on flocculent cells, but sharp on nonflocculent cells. Sexually and asexually flocculent fission-yeast cells cannot be distinguished from one another as both are heavily clad in "mucilaginous" or "hairy" coverings. Examination of lightly extracted and heavily extracted flocculent fission-yeast cells by transmission electron microscopy provides micrographs consistent with the scanning electron micrographs.Key words: flocculation, budding yeast, fission yeast, scanning, transmission.

scholarly journals A FIBER APPARATUS IN THE NUCLEUS OF THE YEAST CELL

1966 ◽  
Vol 29 (1) ◽  
pp. 129-151 ◽  
Author(s):  
C. F. Robinow ◽  
J. Marak
Keyword(s):  
Electron Microscopy ◽  
Light And Electron Microscopy ◽  
Dense Matter ◽  
Short Fiber ◽  
Yeast Cells ◽  
Acid Fuchsin ◽  
Fine Grained ◽  
New Information ◽  
Thin Electron ◽  
Iron Alum

The structure and mode of division of the nucleus of budding yeast cells have been studied by phase-contrast microscopy during life and by ordinary microscopy after Helly fixation. The components of the nucleus were differentially stained by the Feulgen procedure, with Giemsa solution after hydrolysis, and with iron alum haematoxylin. New information was obtained in cells fixed in Helly's by directly staining them with 0.005% acid fuchsin in 1% acetic acid in water. Electron micrographs have been made of sections of cells that were first fixed with 3% glutaraldehyde, then divested of their walls with snail juice, and postfixed with osmium tetroxide. Light and electron microscopy have given concordant information about the organization of the yeast nucleus. A peripheral segment of the nucleus is occupied by relatively dense matter (the "peripheral cluster" of Mundkur) which is Feulgen negative. The greater part of the nucleus is filled with fine-grained Feulgen-positive matter of low density in which chromosomes could not be identified. Chromosomes become visible in this region under the light microscope at meiosis. In the chromatin lies a short fiber with strong affinity for acid fuchsin. The nucleus divides by elongation and constriction, and during this process the fiber becomes long and thin. Electron microscopy has resolved it into a bundle of dark-edged 150 to 180 A filaments which extends between "centriolar plaques" that are attached to the nuclear envelope.

COBALT AS A VITAL STAIN FOR YEAST MITOCHONDRIA IN SQUASH PREPARATIONS

1969 ◽  
Vol 11 (4) ◽  
pp. 987-992 ◽  
Author(s):  
Carl C. Lindegren ◽  
Paraskevi M. Be Miller
Keyword(s):  
Electron Microscopy ◽  
Nutrient Medium ◽  
Yeast Cells ◽  
Yeast Mitochondria ◽  
Inner Membrane ◽  
Vital Stain

Squashes of yeast cells grown in nutrient medium containing cobalt were observed by phase- and electron-microscopy. Co++ is a vital stain that is bound to structures inside the inner membrane of the mitochondria.

A comparison of the effects of several antifungal imidazole derivatives and polyenes on Candida albicans: an ultrastructural study by scanning electron microscopy

1982 ◽  
Vol 28 (10) ◽  
pp. 1119-1126 ◽  
Author(s):  
M. Bastide ◽  
S. Jouvert ◽  
J.-M. Bastide
Keyword(s):  
Electron Microscopy ◽  
Cell Wall ◽  
Candida Albicans ◽  
Cytoplasmic Membrane ◽  
Electron Microscopic Examination ◽  
Yeast Cells ◽  
Electron Microscopic ◽  
Imidazole Derivatives ◽  
Scanning Electron Microscopic ◽  
Scanning Electron

The early events in the interaction of two polyene (amphotericin B and nystatin) and five imidazole (clotrimazole, ketoconazole, miconazole, isoconazole, and econazole) antimycotics used at fungicidal concentrations with the surface of Candida albicans were studied by scanning electron microscopic examination of treated intact young yeast cells, treated spheroplasts, and spheroplasts liberated from treated young yeast cells. In all cases, treatment lasted 2 h. The polyenes passed through the yeast cell wall and interacted with the cytoplasmic membrane causing the spheroplasts to lose their characteristic spheric form and to liberate their contents. Clotrimazole caused the formation of numerous circular openings in the cytoplasmic membrane, but only when the agent was used to treat spheroplasts directly. Ketoconazole, miconazole, isoconazole, and econazole interacted with the cell wall causing formation of convolutions and wrinkles. The three imidazole derivatives that are structurally closely related, miconazole, isoconazole, and econazole, inhibited the enzyme-catalyzed release of spheroplasts from young yeast cells.

scholarly journals Optimization of Innovative Three-Dimensionally-Structured Hybrid Vesicles to Improve the Cutaneous Delivery of Clotrimazole for the Treatment of Topical Candidiasis

Pharmaceutics ◽  
2019 ◽  
Vol 11 (6) ◽  
pp. 263 ◽  
Author(s):  
Maria Letizia Manca ◽  
Iris Usach ◽  
José Esteban Peris ◽  
Antonella Ibba ◽  
Germano Orrù ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Chemical Properties ◽  
Chemical Characterization ◽  
Yeast Cells ◽  
Unilamellar Vesicles ◽  
Transmission Electron ◽  
Physico Chemical

New three-dimensionally-structured hybrid phospholipid vesicles, able to load clotrimazole in a high amount (10 mg/mL), were obtained for the first time in this work by significantly reducing the amount of water (≤10%), which was replaced with a mixture of glycerol and ethanol (≈90%). A pre-formulation study was carried out to evaluate the effect of both the composition of the hydrating medium and the concentration of the phospholipid on the physico-chemical properties of hybrid vesicles. Four different three-dimensionally-structured hybrid vesicles were selected as ideal systems for the topical application of clotrimazole. An extensive physico-chemical characterization performed using transmission electron microscopy (TEM), cryogenic transmission electron microscopy (cryo-TEM), 31P-NMR, and small-angle X-ray scattering (SAXS) displayed the formation of small, multi-, and unilamellar vesicles very close to each other, and was capable of forming a three-dimensional network, which stabilized the dispersion. Additionally, the dilution of the dispersion with water reduced the interactions between vesicles, leading to the formation of single unilamellar vesicles. The evaluation of the in vitro percutaneous delivery of clotrimazole showed an improved drug deposition in the skin strata provided by the three-dimensionally-structured vesicles with respect to the commercial cream (Canesten®) used as a reference. Hybrid vesicles were highly biocompatible and showed a significant antifungal activity in vitro, greater than the commercial cream Canesten®. The antimycotic efficacy of formulations was confirmed by the reduced proliferation of the yeast cells at the site of infection in vivo. In light of these results, clotrimazole-loaded, three-dimensionally-structured hybrid vesicles appear to be one of the most innovative and promising formulations for the treatment of candidiasis infections.

High-Voltage Electron microscopy of Histoplasma capsulatum

1991 ◽  
Vol 49 ◽  
pp. 118-119
Author(s):  
M. J. Song ◽  
M. E. Edwards
Keyword(s):  
Electron Microscopy ◽  
High Voltage ◽  
Histoplasma Capsulatum ◽  
Light Transmission ◽  
Culture Media ◽  
Pathogenic Fungus ◽  
Yeast Cells ◽  
High Voltage Electron Microscopy ◽  
Voltage Electron ◽  
High Voltage Electron

Histoplasma capsulatum (Hc) is a pathogenic fungus frequently involved in lung infections, especially in immunocompromised individuals such as AIDS patients. It has been the subject of numerous investigations by light, transmission, and scanning electron microscopy. However, these studies did not afford a clear picture of the He cell architecture, for which three-dimensional views of the cellular organelles are deemed necessary. For this purpose, we have studied yeast cells of He by means of techniques used in high—voltage electron microscopy (HVEM).Yeast cells of He grown at 37°C in appropriate culture media were fixed with glutaraldehyde-osmium in cacodylate buffer, dehydrated through an ethanol series and embedded in Epon-Araldite, according to standard procedures. Thick sections (0.25-0.5 μm), picked up on Formvar-coated slot-grids, were stained for 4 h at 50°C with uranyl acetate and lead citrate, and examined under the AEI EM7 Mk 1.2 MV Albany HVEM at an accelerating voltage of 1.0 MV.

Enlarged Crystalline Patches on the Plasma Membrane of Yeast Protoplasts

1980 ◽  
Vol 38 ◽  
pp. 686-687
Author(s):  
G. Sosinsky ◽  
R. Schekman ◽  
R. Glaeser
Keyword(s):  
Electron Microscopy ◽  
Cell Wall ◽  
Plasma Membrane ◽  
High Resolution Electron Microscopy ◽  
Yeast Cells ◽  
Physiological Conditions ◽  
Resolution Electron ◽  
Yeast Protoplasts ◽  
Intramembraneous Particles ◽  
Crystalline Array

The crystalline patches of intramembraneous particles that form in the yeast plasma membrane, under stationary state physiological conditions, represent a potentially interesting specimen for high resolution electron microscopy. Isolation of these crystalline membrane patches first requires removal of the cell wall and the formation of osmotically fragile yeast protoplasts. In developing a procedure for the isolation of these crystalline membrane patches, we have found that the intramembraneous particles form much larger crystalline patches in protoplasts than in intact yeast cells. We have performed deep etch experiments and have found that the crystalline array of particles is not expressed on the extracellular surface of the plasma membrane.

ELECTRON MICROSCOPY OF VIRUS-INFECTED YEAST CELLS

1962 ◽  
Vol 83 (6) ◽  
pp. 1363-1364 ◽  
Author(s):  
Tadashi Hirano ◽  
Carl C. Lindegren ◽  
Yong Nyu Bang
Keyword(s):  
Electron Microscopy ◽  
Yeast Cells

Observations on two infectious agents found within the rootlets of the parasitic barnacle, Sacculina carcini

2000 ◽  
Vol 80 (2) ◽  
pp. 373-374 ◽  
Author(s):  
J.D. Russell ◽  
G. Walker ◽  
R. Woollen
Keyword(s):  
Electron Microscopy ◽  
Carcinus Maenas ◽  
Infectious Agent ◽  
Yeast Cells ◽  
Shore Crab ◽  
Infectious Agents ◽  
Transmission Electron ◽  
Sacculina Carcini ◽  
The Common ◽  
Respective Host

Two types of infectious agent within rootlet cells of the parasitic barnacle, Sacculina carcini have been recognized by transmission electron microscopy. The rootlets were dissected from the common shore crab, Carcinus maenas, collected from two locales—Plymouth and Pwllheli. Yeast cells were identified within cells of S. carcini rootlets from crabs collected at both locations and an iridovirus was also found, but only in rootlets from Plymouth crabs. These infectious agents were never found co-occurring in the rootlets from Plymouth crabs. Both agents, when present in rootlets, were also present in the respective host crab tissues. It is therefore concluded that S. carcini rootlets are susceptible to invasion from natural infectious agents of the host crab.

scholarly journals Effects ofMentha suaveolensEssential Oil Alone or in Combination with Other Drugs inCandida albicans

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Annarita Stringaro ◽  
Elisabetta Vavala ◽  
Marisa Colone ◽  
Federico Pepi ◽  
Giuseppina Mignogna ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Candida Albicans ◽  
Fungal Infections ◽  
Antifungal Drugs ◽  
Yeast Cells ◽  
Cellular Damage ◽  
Prolonged Treatment ◽  
Microbial Biofilms ◽  
Transmission Electron ◽  
Morphological Differences

Candidosis is the most important cause of fungal infections in humans. The yeastCandida albicanscan form biofilms, and it is known that microbial biofilms play an important role in human diseases and are very difficult to treat. The prolonged treatment with drugs has often resulted in failure and resistance. Due to the emergence of multidrug resistance, alternatives to conventional antimicrobial therapy are needed. This study aims to analyse the effects induced by essential oil ofMentha suaveolensEhrh (EOMS) onCandida albicansand its potential synergism when used in combination with conventional drugs. Morphological differences between control and EOMS treated yeast cells or biofilms were observed by scanning electron microscopy and transmission electron microscopy (SEM and TEM resp.,). In order to reveal the presence of cell cycle alterations, flow cytometry analysis was carried out as well. The synergic action of EOMS was studied with the checkerboard method, and the cellular damage induced by different treatments was analysed by TEM. The results obtained have demonstrated both the effects of EOMS onC. albicansyeast cells and biofilms and the synergism of EOMS when used in combination with conventional antifungal drugs as fluconazole (FLC) and micafungin (MCFG), and therefore we can hypothesize on its potential use in therapy. Further studies are necessary to know its mechanism of action.

Sign in / Sign up

Export Citation Format

Share Document