Regulation of amyloid precursor mRNA expression by nerve growth factor in the PC12 cell line

1989 ◽  
Vol 1 (1-2) ◽  
pp. 151-151
Author(s):  
S. Ymer ◽  
P. H. Seeburg ◽  
B. D. Shivers
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Mrna Expression ◽  
Cell Line ◽  
Pc12 Cell ◽  
Nerve Growth ◽  
Pc12 Cell Line ◽  
Precursor Mrna

s-Methyl cysteine enhanced survival of nerve growth factor differentiated PC12 cells under hypoxic conditions

2014 ◽  
Vol 5 (6) ◽  
pp. 1125-1133 ◽  
Author(s):  
Chun-lin Liu ◽  
Te-chun Hsia ◽  
Mei-chin Yin
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Cell Line ◽  
Pc12 Cell ◽  
Glucose Deprivation ◽  
Oxygen Glucose Deprivation ◽  
Protective Effects ◽  
Nerve Growth ◽  
Pc12 Cell Line ◽  
Hypoxic Conditions

A nerve growth factor-differentiated PC12 cell line was used to investigate the protective effects ofs-methyl cysteine (SMC) at 1, 2, 4, and 8 μM under oxygen–glucose deprivation (OGD) conditions.

scholarly journals Induction of neurite outgrowth by v-src mimics critical aspects of nerve growth factor-induced differentiation.

1991 ◽  
Vol 11 (9) ◽  
pp. 4739-4750 ◽  
Author(s):  
S M Thomas ◽  
M Hayes ◽  
G D'Arcangelo ◽  
R C Armstrong ◽  
B E Meyer ◽  
...  
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Neurite Outgrowth ◽  
Cell Line ◽  
Pc12 Cells ◽  
Pc12 Cell ◽  
Temperature Sensitive ◽  
Nerve Growth ◽  
Pc12 Cell Line ◽  
Critical Aspects

PC12 cells treated with nerve growth factor (NGF) or infected with Rous sarcoma virus differentiate into sympathetic, neuronlike cells. To compare the differentiation programs induced by NGF and v-src, we have established a PC12 cell line expressing a temperature-sensitive v-src protein. The v-src-expressing PC12 cell line was shown to elaborate neuritic processes in a temperature-inducible manner, indicating that the differentiation process was dependent on the activity of the v-src protein. Further characterization of this cell line, in comparison with NGF-treated PC12 cells, indicated that the events associated with neurite outgrowth induced by these two agents shared features but could be distinguished by others. Both NGF- and v-src-induced neurite outgrowths were reversible. In addition, NGF and v-src could prime PC12 cells for NGF-induced neurite outgrowth, and representative early and late NGF-responsive genes were also induced by v-src. However, unlike NGF-induced neurite growth, v-src-induced neurite outgrowth was not blocked at high cell density. A comparison of phosphotyrosine containing-protein profiles showed that v-src and NGF each increase tyrosine phosphorylation of multiple cellular proteins. There was overlap in substrates; however, both NGF-specific and v-src-specific tyrosine phosphorylations were observed. One protein which was found to be phosphorylated in both the NGF- and v-src-induced PC12 cells was phospholipase C-gamma 1. Taken together, these results suggest that v-src's ability to function as an inducing agent may be a consequence of its ability to mimic critical aspects of the NGF differentiation program and raise the possibility that Src-like tyrosine kinases are involved in mediating some of the events triggered by NGF.

Induction of neurite outgrowth by v-src mimics critical aspects of nerve growth factor-induced differentiation

1991 ◽  
Vol 11 (9) ◽  
pp. 4739-4750
Author(s):  
S M Thomas ◽  
M Hayes ◽  
G D'Arcangelo ◽  
R C Armstrong ◽  
B E Meyer ◽  
...  
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Neurite Outgrowth ◽  
Cell Line ◽  
Pc12 Cells ◽  
Pc12 Cell ◽  
Temperature Sensitive ◽  
Nerve Growth ◽  
Pc12 Cell Line ◽  
Critical Aspects

PC12 cells treated with nerve growth factor (NGF) or infected with Rous sarcoma virus differentiate into sympathetic, neuronlike cells. To compare the differentiation programs induced by NGF and v-src, we have established a PC12 cell line expressing a temperature-sensitive v-src protein. The v-src-expressing PC12 cell line was shown to elaborate neuritic processes in a temperature-inducible manner, indicating that the differentiation process was dependent on the activity of the v-src protein. Further characterization of this cell line, in comparison with NGF-treated PC12 cells, indicated that the events associated with neurite outgrowth induced by these two agents shared features but could be distinguished by others. Both NGF- and v-src-induced neurite outgrowths were reversible. In addition, NGF and v-src could prime PC12 cells for NGF-induced neurite outgrowth, and representative early and late NGF-responsive genes were also induced by v-src. However, unlike NGF-induced neurite growth, v-src-induced neurite outgrowth was not blocked at high cell density. A comparison of phosphotyrosine containing-protein profiles showed that v-src and NGF each increase tyrosine phosphorylation of multiple cellular proteins. There was overlap in substrates; however, both NGF-specific and v-src-specific tyrosine phosphorylations were observed. One protein which was found to be phosphorylated in both the NGF- and v-src-induced PC12 cells was phospholipase C-gamma 1. Taken together, these results suggest that v-src's ability to function as an inducing agent may be a consequence of its ability to mimic critical aspects of the NGF differentiation program and raise the possibility that Src-like tyrosine kinases are involved in mediating some of the events triggered by NGF.

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