Formal integral of a divergence-free system

1995 ◽  
Vol 57 (6) ◽  
pp. 565-572 ◽  
Author(s):  
A. D. Bryuno ◽  
S. Yu. Sadov
Keyword(s):  
Free System ◽  
Formal Integral ◽  
Divergence Free

Shear-free perfect fluids with divergence free magnetic Weyl curvature

2008 ◽  
Vol 30 ◽  
pp. 241-244
Author(s):  
N. Van den Bergh ◽  
H. Reza Karimian
Keyword(s):  
Weyl Curvature ◽  
Perfect Fluids ◽  
Divergence Free

Combination of ribosome and phage display for fast selection of high affinity VHH antibody fragments

2019 ◽  
pp. 27-33
Author(s):  
YuE Kravchenko ◽  
SV Ivanov ◽  
DS Kravchenko ◽  
EI Frolova ◽  
SP Chumakov
Keyword(s):  
Phage Display ◽  
Selection Procedure ◽  
Free System ◽  
Phage Displays ◽  
High Affinity ◽  
Binding Domains ◽  
A Cell ◽  
Vhh Antibodies ◽  
Efficiency Of Selection ◽  
Selection Of

Selection of antibodies using phage display involves the preliminary cloning of the repertoire of sequences encoding antigen-binding domains into phagemid, which is considered the bottleneck of the method, limiting the resulting diversity of libraries and leading to the loss of poorly represented variants before the start of the selection procedure. Selection in cell-free conditions using a ribosomal display is devoid from this drawback, however is highly sensitive to PCR artifacts and the RNase contamination. The aim of the study was to test the efficiency of a combination of both methods, including pre-selection in a cell-free system to enrich the source library, followed by cloning and final selection using phage display. This approach may eliminate the shortcomings of each method and increase the efficiency of selection. For selection, alpaca VHH antibody sequences suitable for building an immune library were used due to the lack of VL domains. Analysis of immune libraries from the genes of the VH3, VHH3 and VH4 families showed that the VHH antibodies share in the VH3 and VH4 gene groups is insignificant, and selection from the combined library is less effective than from the VHH3 family of sequences. We found that the combination of ribosomal and phage displays leads to a higher enrichment of high-affinity fragments and avoids the loss of the original diversity during cloning. The combined method allowed us to obtain a greater number of different high-affinity sequences, and all the tested VHH fragments were able to specifically recognize the target, including the total protein extracts of cell cultures.

Selective Oxidation of Alcohols Catalyzed by a Transition Metal-Free System of NHPI/DDQ/NaNO2

2011 ◽  
Vol 32 (1) ◽  
pp. 118-122 ◽  
Author(s):  
Lipeng ZHOU ◽  
Chaofeng ZHANG ◽  
Tao FANG ◽  
Bingbing ZHANG ◽  
Ying WANG ◽  
...  
Keyword(s):  
Transition Metal ◽  
Selective Oxidation ◽  
Free System ◽  
Metal Free ◽  
Oxidation Of Alcohols

Isolation of globin mRNA from spleen of anemic rabbits and its translation in wheat embryo cell-free system

1978 ◽  
Vol 43 (4) ◽  
pp. 1184-1189
Author(s):  
Ota Fuchs ◽  
Jitka Borová ◽  
Přemysl Poňka ◽  
Jan Neuwirt
Keyword(s):  
Embryo Cell ◽  
Globin Mrna ◽  
Free System ◽  
Wheat Embryo ◽  
Cell Free System
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