Assay of extracellular proteinases using a colorimetric collagen substrate for the differentiation ofSerratia in the tribe Klebsielleae

1985 ◽  
Vol 174 (3) ◽  
pp. 115-118
Author(s):  
S. C. Edberg ◽  
M. K. Edberg
Keyword(s):  
Collagen Substrate ◽  
Extracellular Proteinases

Electron microscopy of keratinocytes cultured on a collagen substrate used as an allograft for the treatment of chronic wounds

1992 ◽  
Vol 50 (2) ◽  
pp. 1104-1105
Author(s):  
Debby A. Jennings ◽  
Michael J. Morykwas ◽  
Louis C. Argenta
Keyword(s):  
Electron Microscopy ◽  
Cell Suspension ◽  
Single Cell ◽  
Chronic Wounds ◽  
Mechanical Stability ◽  
Uv Light ◽  
Type I ◽  
Single Cell Suspension ◽  
Collagen Substrate ◽  
Dermal Collagen

Grafts of cultured allogenic or autogenic keratlnocytes have proven to be an effective treatment of chronic wounds and burns. This study utilized a collagen substrate for keratinocyte and fibroblast attachment. The substrate provided mechanical stability and augmented graft manipulation onto the wound bed. Graft integrity was confirmed by light and transmission electron microscopy.Bovine Type I dermal collagen sheets (100 μm thick) were crosslinked with 254 nm UV light (13.5 Joules/cm2) to improve mechanical properties and reduce degradation. A single cell suspension of third passage neonatal foreskin fibroblasts were plated onto the collagen. Five days later, a single cell suspension of first passage neonatal foreskin keratinocytes were plated on the opposite side of the collagen. The grafts were cultured for one month.The grafts were fixed in phosphate buffered 4% formaldehyde/1% glutaraldehyde for 24 hours. Graft pieces were then washed in 0.13 M phosphate buffer, post-fixed in 1% osmium tetroxide, dehydrated, and embedded in Polybed 812.

scholarly journals SPR imaging study of DNA wrapped single wall carbon nanotube (ssDNA-SWCNT) adsorption on a model biological (collagen) substrate

Soft Matter ◽  
2010 ◽  
Vol 6 (21) ◽  
pp. 5581 ◽  
Author(s):  
Jung Jin Park ◽  
Jeffrey A. Fagan ◽  
Ji Yeon Huh ◽  
Kalman B. Migler ◽  
Alamgir Karim ◽  
...  
Keyword(s):  
Carbon Nanotube ◽  
Imaging Study ◽  
Single Wall Carbon Nanotube ◽  
Single Wall Carbon ◽  
Collagen Substrate ◽  
Spr Imaging

Self-Assembly of Collagen on Flat Surfaces: The Interplay of Collagen–Collagen and Collagen–Substrate Interactions

Langmuir ◽  
2014 ◽  
Vol 30 (5) ◽  
pp. 1343-1350 ◽  
Author(s):  
Badri Narayanan ◽  
George H. Gilmer ◽  
Jinhui Tao ◽  
James J. De Yoreo ◽  
Cristian V. Ciobanu
Keyword(s):  
Self Assembly ◽  
Substrate Interactions ◽  
Flat Surfaces ◽  
Collagen Substrate

Long-term growth of chicken fibroblasts on a collagen substrate

1974 ◽  
Vol 84 (1-2) ◽  
pp. 63-71 ◽  
Author(s):  
G.O. Gey ◽  
M. Svotelis ◽  
M. Foard ◽  
F.B. Bang
Keyword(s):  
Collagen Substrate

Secretion of Extracellular Proteinases Active against Fibrillar Proteins by Micromycetes

2017 ◽  
Vol 72 (4) ◽  
pp. 206-210
Author(s):  
E. A. Popova ◽  
D. M. Bednenko ◽  
A. A. Osmolovskiy ◽  
V. G. Kreyer ◽  
I. B. Kotova ◽  
...  
Keyword(s):  
Extracellular Proteinases

Type VI collagen induces cardiac myofibroblast differentiation: implications for postinfarction remodeling

2006 ◽  
Vol 290 (1) ◽  
pp. H323-H330 ◽  
Author(s):  
Jennifer E. Naugle ◽  
Erik R. Olson ◽  
Xiaojin Zhang ◽  
Sharon E. Mase ◽  
Charles F. Pilati ◽  
...  
Keyword(s):  
Cardiac Fibrosis ◽  
Type I Collagen ◽  
In Vivo Model ◽  
Type Iii Collagen ◽  
Type I ◽  
Myofibroblast Differentiation ◽  
Type Vi Collagen ◽  
Collagen Substrate ◽  
Type Iii

Cardiac fibroblast (CF) proliferation and differentiation into hypersecretory myofibroblasts can lead to excessive extracellular matrix (ECM) production and cardiac fibrosis. In turn, the ECM produced can potentially activate CFs via distinct feedback mechanisms. To assess how specific ECM components influence CF activation, isolated CFs were plated on specific collagen substrates (type I, III, and VI collagens) before functional assays were carried out. The type VI collagen substrate potently induced myofibroblast differentiation but had little effect on CF proliferation. Conversely, the type I and III collagen substrates did not affect differentiation but caused significant induction of proliferation (type I, 240.7 ± 10.3%, and type III, 271.7 ± 21.8% of basal). Type I collagen activated ERK1/2, whereas type III collagen did not. Treatment of CFs with angiotensin II, a potent mitogen of CFs, enhanced the growth observed on types I and III collagen but not on the type VI collagen substrate. Using an in vivo model of myocardial infarction (MI), we measured changes in type VI collagen expression and myofibroblast differentiation after post-MI remodeling. Concurrent elevations in type VI collagen and myofibroblast content were evident in the infarcted myocardium 20-wk post-MI. Overall, types I and III collagen stimulate CF proliferation, whereas type VI collagen plays a potentially novel role in cardiac remodeling through facilitation of myofibroblast differentiation.

scholarly journals Heterogeneity of metallo and serine extracellular proteinases in oral clinical isolates ofCandida albicansin HIV-positive and healthy children from Rio de Janeiro, Brazil

2003 ◽  
Vol 38 (2) ◽  
pp. 173-180 ◽  
Author(s):  
Edja Maria Melo de Brito Costa ◽  
André Luis Souza Santos ◽  
Abel Silveira Cardoso ◽  
Maristela Barbosa Portela ◽  
Celina Monteiro Abreu ◽  
...  
Keyword(s):  
Rio De Janeiro ◽  
Clinical Isolates ◽  
Hiv Positive ◽  
Healthy Children ◽  
Extracellular Proteinases

scholarly journals Inter- and Intracellular luminal formation in porcine thyroid tissues cultured in a collagen substrate.

1989 ◽  
Vol 52 (2) ◽  
pp. 109-114 ◽  
Author(s):  
Keisuke YAMASHITA ◽  
Hisao FUJITA ◽  
Yoshio NISHII
Keyword(s):  
Collagen Substrate

Extracellular proteinases from the phytopathogenic fungus Fusarium culmorum

2006 ◽  
Vol 42 (3) ◽  
pp. 298-303 ◽  
Author(s):  
E. V. Ievleva ◽  
T. A. Revina ◽  
N. N. Kudryavtseva ◽  
A. V. Sof’in ◽  
T. A. Valueva
Keyword(s):  
Fusarium Culmorum ◽  
Phytopathogenic Fungus ◽  
Extracellular Proteinases
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