Ultrastructural view of central catecholaminergic transmission: immunocytochemical localization of synthesizing enzymes, transporters and receptors

1996 ◽  
Vol 25 (1) ◽  
pp. 843-856 ◽  
Author(s):  
Virginia M. Pickel ◽  
Melissa J. Nirenberg ◽  
Teresa A. Milner
Keyword(s):  
Immunocytochemical Localization ◽  
Catecholaminergic Transmission

Immunocytochemical localization of p65 with one-nanometer gold particles following silver development

1989 ◽  
Vol 47 ◽  
pp. 1042-1043
Author(s):  
Richard W. Burry ◽  
Diane M. Hayes
Keyword(s):  
Plasma Membrane ◽  
Bovine Serum Albumin ◽  
Calf Serum ◽  
Specific Protein ◽  
Immunocytochemical Localization ◽  
Electron Microscopic ◽  
Gold Particles ◽  
Pass Through ◽  
Label Distribution ◽  
Phosphate Buffered Saline

Electron microscopic (EM) immunocytochemistry localization of the neuron specific protein p65 could show which organelles contain this antigen. Antibodies (Ab) labeled with horseradish peroxidase (HRP) followed by chromogen development show a broad diffuse label distribution within cells and restricting identification of organelles. Particulate label (e.g. 10 nm colloidal gold) is highly desirable but not practical because penetration into cells requires destroying the plasma membrane. We report pre-embedding immunocytochemistry with a particulate marker, 1 nm gold, that will pass through membranes treated with saponin, a mild detergent.Cell cultures of the rat cerebellum were fixed in buffered 4% paraformaldehyde and 0.1% glutaraldehyde (Glut.). The buffer for all incubations and rinses was phosphate buffered saline with: 1% calf serum, 0.2% saponin, 0.1% gelatin, 50 mM glycine 1 mg/ml bovine serum albumin, and (not in the HRP labeled cultures) 0.02% sodium azide. The monoclonal #48 to p65 was used with three label systems: HRP, 1 nm avidin gold with IntenSE M development, and 1 nm avidin gold with Danscher development.

Immunocytochemical localization of desmin and vimentin in chick embryonic myocardial cells

1990 ◽  
Vol 48 (3) ◽  
pp. 448-449
Author(s):  
Yukiko Sugi
Keyword(s):  
Sodium Methoxide ◽  
Intermediate Filament Protein ◽  
Chick Embryos ◽  
Immunocytochemical Localization ◽  
Myocardial Cells ◽  
Immunofluorescence Study ◽  
Immunofluorescent Localization ◽  
Rabbit Igg ◽  
Semithin Sections ◽  
Filament Protein

In cultured skeletal muscle cells of chick, one intermediate filament protein, vimentin, is primarily formed and then synthesis of desmin follows. Coexistence of vimentin and desmin has been immunocytochemically confirmed in chick embryonic skeletal musclecells. Immunofluorescent localization of vimentin and desmin has been described in developing myocardial cells of hamster. However, initial localization of desmin and vimentin in early embryonic heart has not been reported in detail. By quick-freeze deep-etch method a loose network of intermediate filaments was revealed to exist surrounding myofibrils. In this report, immunocytochemical localization of desmin and vimentin is visualized in early stages of chick embryonic my ocardium.Chick embryos, Hamburger-Hamilton (H-H) stage 8 to hatch, and 1 day old postnatal chicks were used in this study. For immunofluorescence study, each embryo was fixed with 4% paraformaldehyde and embedded in Epon 812. De-epoxinized with sodium methoxide, semithin sections were stained with primary antibodies (rabbit anti-desmin antibody and anti-vimentin antibody)and secondary antibody (RITC conjugated goat-anti rabbit IgG).

Immunocytochemical Localization of 5α-Reductase in Rat Brain

1994 ◽  
Vol 5 (5) ◽  
pp. 394-399 ◽  
Author(s):  
Georges Pelletier ◽  
Van Luu-The ◽  
Fernand Labrie
Keyword(s):  
Rat Brain ◽  
Immunocytochemical Localization

Immunocytochemical localization of the GABAB2 receptor subunit in the glomeruli of the mouse main olfactory bulb

2006 ◽  
Vol 402 (1-2) ◽  
pp. 121-125 ◽  
Author(s):  
Igor Kratskin ◽  
Natalia Kenigfest ◽  
Jean Paul Rio ◽  
Chakib Djediat ◽  
Jacques Repérant
Keyword(s):  
Olfactory Bulb ◽  
Immunocytochemical Localization ◽  
Receptor Subunit ◽  
Main Olfactory Bulb

Biochemical and Immunocytochemical Localization of Heat-Shock Proteins in Drosophila Cultured Cells

1985 ◽  
Vol 455 (1 Intermediate) ◽  
pp. 712-714 ◽  
Author(s):  
ROBERT M. TANGUAY ◽  
JEAN-LOUP DUBAND ◽  
FRANCINE LETTRE ◽  
JEAN-PAUL VALET ◽  
ANDRÉ PATRICK ARRIGO ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Proteins ◽  
Cultured Cells ◽  
Immunocytochemical Localization ◽  
Shock Proteins

Immunocytochemical Localization of Thyroid Hormone Receptors in the Adult Rat Brain

Thyroid ◽  
1991 ◽  
Vol 1 (2) ◽  
pp. 173-184 ◽  
Author(s):  
J. PUYMIRAT ◽  
M. MIEHE ◽  
R. MARCHAND ◽  
L. SARLIEVE ◽  
J.H. DUSSAULT
Keyword(s):  
Thyroid Hormone ◽  
Rat Brain ◽  
Hormone Receptors ◽  
Thyroid Hormone Receptors ◽  
Immunocytochemical Localization ◽  
Adult Rat ◽  
Adult Rat Brain
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