Tetrasomy 18p de novo: Identification by FISH with conventional and microdissection probes and analysis of parental origin and formation by short sequence repeat typing

1996 ◽  
Vol 97 (5) ◽  
pp. 568-572 ◽  
Author(s):  
Thomas Eggermann ◽  
Hartmut Engels ◽  
Barbara Moskalonek ◽  
Markus M. Nöthen ◽  
Jutta Müller-Navia ◽  
...  
Keyword(s):  
De Novo ◽  
Parental Origin ◽  
Short Sequence ◽  
Sequence Repeat ◽  
Short Sequence Repeat ◽  
Tetrasomy 18P

Tetrasomy 18p de novo: identification by FISH with conventional and microdissection probes and analysis of parental origin and formation by short sequence repeat typing

1996 ◽  
Vol 97 (5) ◽  
pp. 568-572 ◽  
Author(s):  
Thomas Eggermann ◽  
H. Engels ◽  
Barbara Moskalonek ◽  
Markus M. Nöthen ◽  
Jutta Müller-Navia ◽  
...  
Keyword(s):  
De Novo ◽  
Parental Origin ◽  
Short Sequence ◽  
Sequence Repeat ◽  
Short Sequence Repeat ◽  
Tetrasomy 18P

Applications of microsatellite markers in studies of genetics and breeding of fish

2006 ◽  
Vol 3 (2) ◽  
pp. 83-87 ◽  
Author(s):  
Zhang Xiao-Gu ◽  
Tong Jin-Gou ◽  
Xiong Bang-Xi
Keyword(s):  
Genetic Diversity ◽  
Genetic Structure ◽  
Population Genetic ◽  
Germplasm Conservation ◽  
Genomic Mapping ◽  
Short Sequence ◽  
Sequence Repeat ◽  
Short Sequence Repeat ◽  
Food Fish ◽  
Polymorphic Microsatellite

AbstractThe microsatellite, or short sequence repeat (SSR), is a powerful genetic marker, useful in many areas of fish genetics and breeding. Polymorphic microsatellite loci have been frequently applied to the analysis of genetic diversity, population genetic structure, and genomic mapping. These co-dominant markers have also been applied to the classification and systematics, parentage identification, germplasm conservation, and breeding programme of food fish.

scholarly journals RepeatAnalyzer: a tool for analysing and managing short-sequence repeat data

BMC Genomics ◽  
2016 ◽  
Vol 17 (1) ◽  
Author(s):  
Helen N. Catanese ◽  
Kelly A. Brayton ◽  
Assefaw H. Gebremedhin
Keyword(s):  
Short Sequence ◽  
Sequence Repeat ◽  
Short Sequence Repeat

Tetrasomy 18p de novo: Parental Origin and Different Mechanisms of Formation

1996 ◽  
Vol 4 (3) ◽  
pp. 160-167 ◽  
Author(s):  
Merete Bugge ◽  
Elisabeth Blennow ◽  
Ursula Friedrich ◽  
Michael B. Petersen ◽  
Florence Pedeutour ◽  
...  
Keyword(s):  
De Novo ◽  
Parental Origin ◽  
Tetrasomy 18P ◽  
Mechanisms Of Formation

scholarly journals Simple Sequence Repeat Markers Distinguish among Morphotypes of Sphaeropsis sapinea

2001 ◽  
Vol 67 (1) ◽  
pp. 354-362 ◽  
Author(s):  
Treena Burgess ◽  
Michael J. Wingfield ◽  
Brenda W. Wingfield
Keyword(s):  
Pcr Amplification ◽  
Fungal Endophyte ◽  
Polymorphic Markers ◽  
Short Sequence ◽  
Sequence Repeat ◽  
Short Sequence Repeat ◽  
Geographic Origins ◽  
Natural Range ◽  
Botryosphaeria Obtusa ◽  
Sphaeropsis Sapinea

ABSTRACT Sphaeropsis sapinea is a fungal endophyte ofPinus spp. that can cause disease following predisposition of trees by biotic or abiotic stresses. Four morphotypes of S. sapinea have been described from within the natural range of the fungus, while only one morphotype has been identified on exotic pines in the Southern Hemisphere. The aim of this study was to develop robust polymorphic markers that could be used in both taxonomic and population studies. Inter-short-sequence-repeat primers containing microsatellite sequences and degenerate anchors at the 5′ end were used to target microsatellite-rich areas in an S. sapinea isolate. PCR amplification using an annealing temperature of 49°C resulted in profiles containing 5 to 10 bands. These bands were cloned and sequenced, and new short-sequence-repeat (SSR) primer pairs were designed that flanked microsatellite-rich regions. Eleven polymorphic SSR markers were tested on 40 isolates of S. sapinearepresenting different morphotypes as well as on 2 isolates of the closely related species Botryosphaeria obtusa. The putative I morphotype was found to be identical to B. obtusa. Otherwise, the markers clearly distinguished the remaining three morphotypes and, furthermore, showed that the C morphotype was more closely related to the A than the B morphotype. The B morphotype was the most genetically diverse, and the isolates could be further divided based on their geographic origins. Sequencing of different alleles from each locus showed that the most polymorphic markers had mutations within a microsatellite sequence.

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