Efficiency in packed column supercritical fluid chromatography using a modified mobile phase

1991 ◽  
Vol 31 (11-12) ◽  
pp. 529-534 ◽  
Author(s):  
T. A. Berger ◽  
J. F. Deye
Keyword(s):  
Supercritical Fluid ◽  
Supercritical Fluid Chromatography ◽  
Mobile Phase ◽  
Packed Column

Supercritical argon as a mobile phase for the flame photometric detection of sulfur

2003 ◽  
Vol 81 (10) ◽  
pp. 1051-1056 ◽  
Author(s):  
Kevin B Thurbide ◽  
Brad W Cooke
Keyword(s):  
Carbon Dioxide ◽  
Supercritical Carbon Dioxide ◽  
Supercritical Fluid ◽  
Supercritical Fluid Chromatography ◽  
Mobile Phase ◽  
Packed Column ◽  
Flow Rates ◽  
Photometric Detection ◽  
Flame Photometric Detection ◽  
Background Emission

The background emission properties of supercritical argon and supercritical carbon dioxide mobile phases in packed column supercritical fluid chromatography (pSFC) with flame photometric detection (FPD) were compared. As column flow rates were increased toward common values used in pSFC, the carbon dioxide background emission grew enormously. The resulting emission spectrum displayed dominant features at wavelengths between 325 and 525 nm, consisting of a complex series of overlapping molecular emission band systems partly attributed to CO* and CH*. By comparison, when using the same flow rates with a supercritical argon mobile phase, the background emission was identical to that of the FPD flame without column effluent. In terms of intensity, when using a column flow rate of 2 mL/min, supercritical carbon dioxide contributes a background emission in the FPD that is about 3 × 105 times larger than that produced by supercritical argon. This difference leads to an improvement of two orders of magnitude in the pSFC-FPD signal-to-noise ratio for sulfur when a supercritical argon mobile phase is used. Results indicate that supercritical argon could also be advantageous for the pSFC-FPD analysis of other elements.Key words: supercritical fluid chromatography, packed column, flame photometric detection, supercritical argon.

scholarly journals Determination of Antifungals in Rodent Diet by Supercritical Fluid Extraction Followed by Packed Column Supercritical Fluid Chromatography with Ultraviolet Detection

1997 ◽  
Vol 80 (1) ◽  
pp. 7-13 ◽  
Author(s):  
John R Dean ◽  
Ian A Fowlis ◽  
Stephen M Hitchen ◽  
Sharmin Khundker ◽  
Edwin Ludkin ◽  
...  
Keyword(s):  
Supercritical Fluid Extraction ◽  
Supercritical Fluid ◽  
Supercritical Fluid Chromatography ◽  
Mobile Phase ◽  
Packed Column ◽  
Fluid Extraction ◽  
Ultraviolet Detection ◽  
Silica Column ◽  
Column Pressure

Abstract Supercritical fluid extraction (SFE) followed by packed column supercritical fluid chromatography with ultraviolet detection was evaluated as a quantitative method for determining 4 antifungals (fluconazole, tioconazole, hexaconazole, and UK- 47,265) in rodent diet. Chromatography was achieved with a cyano-bonded silica column, UV detection at 210 nm, and methanol-modified supercritical carbon dioxide as mobile phase. The effects of modifier concentration, temperature, and column pressure on antifungal retention time was studied. Off-line SFE was optimized at 2 spike levels, ranging from 0.5 to 10 g/kg, for each of the 4 antifungals. Average recoveries ranged from 79.0% for UK-47,265 to 96.5% for hexaconazole. Overall, the procedure provides a suitable method for analyzing antifungals in spiked rodent diet.

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