The preparation of analytically stable, immobilized stationary phase coatings on colums packing materials

1984 ◽  
Vol 18 (1) ◽  
pp. 11-14 ◽  
Author(s):  
L. Ghaoui ◽  
H. Shanfield ◽  
A. Zlatkis
Keyword(s):  
Stationary Phase ◽  
Packing Materials ◽  
Immobilized Stationary Phase

Effect of stationary phase structure on retention and selectivity of restricted-access reversed-phase packing materials

1991 ◽  
Vol 558 (1) ◽  
pp. 19-30 ◽  
Author(s):  
Kazuhiro Kimata ◽  
Ken Hosoya ◽  
Nobuo Tanaka ◽  
Takeo Araki ◽  
Riyou Tsuboi ◽  
...  
Keyword(s):  
Stationary Phase ◽  
Phase Structure ◽  
Reversed Phase ◽  
Packing Materials ◽  
Restricted Access ◽  
Reversed Phase Packing

Immobilized Stationary Phase (Material) (In Chromatography)

2019 ◽  
Author(s):  
Tatiana A. Maryutina ◽  
Elena Yu. Savonina ◽  
Petr S. Fedotov ◽  
Roger M. Smith ◽  
Heli Siren ◽  
...  
Keyword(s):  
Stationary Phase ◽  
Immobilized Stationary Phase

scholarly journals Synthesis and application of stationary phase for DNA-affinity chromatographic analysis of unmodified and antisense oligonucleotide

2021 ◽  
Author(s):  
Sylwia Studzińska ◽  
Ewelina Zawadzka ◽  
Szymon Bocian ◽  
Michał Szumski
Keyword(s):  
Liquid Chromatography ◽  
Stationary Phase ◽  
Affinity Chromatography ◽  
Antisense Oligonucleotide ◽  
Antisense Oligonucleotides ◽  
Stationary Phases ◽  
Exchange Chromatography ◽  
Oligonucleotide Hybridization ◽  
Immobilized Stationary Phase ◽  
Pentanedioic Acid

AbstractThe goal of the research was the synthesis and application of an oligonucleotide immobilized stationary phase for the analysis of unmodified and antisense oligonucleotides. The method for attaching these molecules to aminopropyl silica modified with pentanedioic acid was developed. Each step of the synthesis was carefully controlled with the application of spectroscopic, elemental, and chromatographic analyses. The oligonucleotide-based stationary phase was applied for the retention studies. Unmodified oligonucleotides of different complementarity to the molecule attached as a stationary phase, as well as antisense oligonucleotides, were tested. The comparative study upon complex optimization of oligonucleotide analysis in different liquid chromatography modes was performed. Results have shown that this stationary phase may be applied for oligonucleotide analysis in hydrophilic interaction liquid chromatography and ion exchange chromatography, but no unique sequence-based selectivity was obtained. Contrary results were observed for affinity chromatography, which allowed for specific separation of the complementary strands based on hydrogen bonding and stacking interactions, where the temperature was the main factor influencing the selectivity of the separation. Furthermore, the oligonucleotide-based stationary phase may be applied for comparative antisense oligonucleotide hybridization studies to a specific RNA sequence. All of the results have shown that affinity chromatography with oligonucleotide-based stationary phases is a powerful technique for the specific base recognition of polynucleotides. Graphical abstract

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