Instrumental liquid chromatography—A practical manual on high performance liquid chromatographic methods

A highly sensitive method for the separation and quantitative measurement of phospholipids containing primary amino groups, such as phosphatidylethanolamine, phosphatidylserine and lysophosphatidylethanolamine, is described. The method involves a simple and quantitative derivative formation of the phospholipids containing amino groups to their u.v.-absorbing biphenylcarbonyl derivatives. These have molar extinction coefficients of about 23,000 at 268nm. The phospholipid derivatives are then separated and non-destructively determined by high-performance liquid chromatography. The amino phospholipids containing vinyl ether bonds (plasmalogens) can be determined separately from the diacyl- and alkylacyl-amino phospholipids. The lower limit of detection by high-performance liquid-chromatographic analysis of the phospholipid derivatives is about 10-13pmol or 0.3-0.4ng of phospholipid P. The quantitative range of derivative formation and analysis by high-performance liquid chromatography of the phospholipids containing amino groups was shown to be 10-500nmol. The method was shown to be applicable to the analysis of phospholipids containing amino groups in tissue samples.

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Thin-layer and high performance liquid chromatographic methods for the analysis of aflatoxins in animal tissues and fluids

Diagnosis of Mycotoxicoses ◽

10.1007/978-94-009-4235-6_22 ◽

1986 ◽

pp. 257-269 ◽

Cited By ~ 3

Author(s):

Robert D. Stubblefield

Keyword(s):

Thin Layer ◽

High Performance ◽

High Performance Liquid Chromatographic ◽

Animal Tissues ◽

Chromatographic Methods ◽

Liquid Chromatographic

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Application of "high-performance" liquid chromatography to the study of sphingolipidoses.

Clinical Chemistry ◽

10.1093/clinchem/26.10.1499 ◽

1980 ◽

Vol 26 (10) ◽

pp. 1499-1503 ◽

Cited By ~ 15

Author(s):

M D Ullman ◽

R E Pyeritz ◽

H W Moser ◽

D A Wenger ◽

E H Kolodny

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Plasma Exchange ◽

Chromatographic Analysis ◽

High Performance ◽

High Performance Liquid Chromatographic ◽

Reversed Phase ◽

Urine Sediment ◽

Liquid Chromatographic Analysis ◽

Liquid Chromatographic

Abstract Quantitative high-performance liquid chromatographic analysis of perbenzoylated sphingolipids has been used to study the correlations of body chemistry to clinical phenomena. Plasma sphingolipids were isolated from 32 Gaucher (β-glucosidase deficiency) and six Fabry (α-galactosidase deficiency) patients by solvent partition and chromatographic separation on silicic acid columns. Plasma sphingolipids from a patient undergoing plasma-exchange were separated from interfering lipids with reversed-phase columns. Liquid-chromatographic analysis of sphingolipids provides useful supportive information for diagnoses because affected individuals are shown to possess increased circulating concentrations of the pathognomonic sphingolipid. We also used this technique to monitor sphingolipid concentrations in plasma and urine sediment during plasma exchange of a p atient with Fabry’s disease. Regular plasma exchanges produced and maintained decreased concentrations of sphingolipids in plasma, but near pre-exchange concentrations were observed within days after the therapy was terminated.

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