Single step LC method for determination of folypolyglutamate synthetase activity and separation of polyglutamates

1993 ◽  
Vol 37 (1-2) ◽  
pp. 73-78 ◽  
Author(s):  
C. Montero ◽  
P. Llorente
Keyword(s):  
Single Step ◽  
Synthetase Activity

scholarly journals A single-step purification method for the precise determination of the antimony isotopic composition of environmental, geological and biological samples by HG-MC-ICP-MS

2021 ◽  
Author(s):  
Ferrari Colin ◽  
Resongles Eléonore ◽  
Freydier Rémi ◽  
Casiot Corinne
Keyword(s):  
Isotopic Composition ◽  
Biological Samples ◽  
Precise Determination ◽  
Single Step ◽  
Functionalized Silica ◽  
Purification Method ◽  
Silica Powder ◽  
Icp Ms ◽  
Single Step Purification

Thiol-functionalized silica powder allowed single-step purification of antimony for exploring stable Sb isotope signatures in the environment.

scholarly journals Acyl-CoA synthetase and the peroxisomal enzymes of β-oxidation in human liver. Quantitative analysis of their subcellular localization

1984 ◽  
Vol 224 (3) ◽  
pp. 709-720 ◽  
Author(s):  
M Bronfman ◽  
N C Inestrosa ◽  
F O Nervi ◽  
F Leighton
Keyword(s):  
Human Liver ◽  
Fatty Acyl ◽  
Single Step ◽  
Synthetase Activity ◽  
Beta Oxidation ◽  
Analysis Of Results ◽  
Fractionation Method ◽  
Hydroxyacyl Coa Dehydrogenase ◽  
Acyl Coa Oxidase ◽  
Rate Limiting

The presence of acyl-CoA synthetase (EC 6.2.1.3) in peroxisomes and the subcellular distribution of beta-oxidation enzymes in human liver were investigated by using a single-step fractionation method of whole liver homogenates in metrizamide continuous density gradients and a novel procedure of computer analysis of results. Peroxisomes were found to contain 16% of the liver palmitoyl-CoA synthetase activity, and 21% and 60% of the enzyme activity was localized in mitochondria and microsomal fractions respectively. Fatty acyl-CoA oxidase was localized exclusively in peroxisomes, confirming previous results. Human liver peroxisomes were found to contribute 13%, 17% and 11% of the liver activities of crotonase, beta-hydroxyacyl-CoA dehydrogenase and thiolase respectively. The absolute activities found in peroxisomes for the enzymes investigated suggest that in human liver fatty acyl-CoA oxidase is the rate-limiting enzyme of the peroxisomal beta-oxidation pathway, when palmitic acid is the substrate.

Single step method for the accurate concentration determination of polysorbate 80

1997 ◽  
Vol 786 (1) ◽  
pp. 99-106 ◽  
Author(s):  
Travis H. Tani ◽  
Jamie M. Moore ◽  
Thomas W. Patapoff
Keyword(s):  
Single Step ◽  
Polysorbate 80 ◽  
Step Method ◽  
Concentration Determination

scholarly journals Simultaneous determination of iron and copper in children's sera by FAAS

2011 ◽  
Vol 61 (1) ◽  
pp. 93-102 ◽  
Author(s):  
Svjetlana Luterotti ◽  
Tončica Kordić ◽  
Slavica Dodig
Keyword(s):  
Atomic Absorption ◽  
Simultaneous Determination ◽  
Sample Pretreatment ◽  
Single Step ◽  
Design Experiment ◽  
New Approach ◽  
Flame Atomic Absorption ◽  
Reference Methods ◽  
Atomic Absorption Spectrometric

Simultaneous determination of iron and copper in children's sera by FAASA new and simple flame atomic-absorption spectrometric (FAAS) method is proposed for simultaneous determination of iron and copper in children's sera. It is based on single-step sample pretreatment (deproteinization with 3 mol L-1HCl, ratio 1:1) and single-step calibration using 1.5 mol L-1HCl standard. During method's optimization a short multifactorial design experiment was used. The proposed method assures accuracy, sensitivity and precision comparable to that of the reference methods. The new approach is simple and time-, labour- and serum-saving, the latter being especially important in pediatric diagnostics.

scholarly journals On the determination of step energies. Theoretical considerations and application to an anisotropic Kossel model

2006 ◽  
Vol 39 (4) ◽  
pp. 563-570 ◽  
Author(s):  
M. A. Deij ◽  
J. H. Los ◽  
H. Meekes ◽  
E. Vlieg
Keyword(s):  
Lattice Models ◽  
Single Step ◽  
Spiral Growth ◽  
Growth Theory ◽  
Two Dimensional ◽  
Island Growth ◽  
Growth Step ◽  
Step Flow ◽  
Theoretical Considerations

Steps on surfaces are important in crystal growth theory, as the step free energy determines the two-dimensional nucleation rate, island growth, step flow and spiral growth. In this paper, it is illustrated that in general in lattice models the step energy of a single step cannot be determined directly by counting broken bonds. A new method is proposed that uses the geometry of a step together with the bonding topology, allowing for a straightforward determination of single-step energies for any case. The method is applied to an anisotropic Kossel model.

scholarly journals Correction: A single-step purification method for the precise determination of the antimony isotopic composition of environmental, geological and biological samples by HG-MC-ICP-MS

2021 ◽  
Author(s):  
Colin Ferrari ◽  
Eléonore Resongles ◽  
Rémi Freydier ◽  
Corinne Casiot
Keyword(s):  
Isotopic Composition ◽  
Biological Samples ◽  
Precise Determination ◽  
Single Step ◽  
Purification Method ◽  
Icp Ms ◽  
Single Step Purification

Correction for ‘A single-step purification method for the precise determination of the antimony isotopic composition of environmental, geological and biological samples by HG-MC-ICP-MS’ by Colin Ferrari et al., J. Anal. At. Spectrom., 2021, 36, 776–785, DOI: "https://doi.org/10.1039/D0JA00452A">10.1039/D0JA00452A.

Gas-chromatographic determination of cholesterol sulfate in plasma and erythrocytes, for the diagnosis of recessive X-linked ichthyosis.

1983 ◽  
Vol 29 (7) ◽  
pp. 1404-1407 ◽  
Author(s):  
F A Muskiet ◽  
G Jansen ◽  
B G Wolthers ◽  
A Marinkovic-Ilsen ◽  
P C van Voorst Vader
Keyword(s):  
Internal Standard ◽  
Dehydroepiandrosterone Sulfate ◽  
Chromatographic Determination ◽  
Anion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Single Step ◽  
Flame Ionization Detection ◽  
Cholesterol Sulfate ◽  
Flame Ionization

Abstract We describe a rapid method for determining cholesterol sulfate in plasma and erythrocytes. After its single-step isolation by means of anion-exchange chromatography cholesterol sulfate is hydrolyzed, trimethylsilylated, and determined by gas chromatography with flame ionization detection. 5 beta-Cholestan-3 alpha-ol sulfate is used as internal standard. The method enables simultaneous determination of dehydroepiandrosterone sulfate in plasma. We applied it for the diagnosis of seven patients with recessive X-linked ichthyosis. Concentrations are given for plasma and erythrocytes from four unaffected relatives of patients with X-linked ichthyosis, a patient with placental sulfatase deficiency, two patients with other types of ichthyoses, and 20 controls. The method may also be of use for the rapid isolation of other organic sulfates from biological material, as illustrated by a comparison of gas chromatograms of urine from a normal pregnant woman and that from a patient with placental sulfatase deficiency.

scholarly journals Determination of Insecticides in Honey by Matrix Solid-Phase Dispersion and Gas Chromatography with Nitrogen–Phosphorus Detection and Mass Spectrometric Confirmation

2002 ◽  
Vol 85 (1) ◽  
pp. 128-133 ◽  
Author(s):  
Consuelo Sánchez-Brunete ◽  
Beatriz Albero ◽  
Ester Miguel ◽  
José Luis Tadeo
Keyword(s):  
Solid Phase ◽  
Single Step ◽  
Organophosphorus Insecticides ◽  
Matrix Solid Phase Dispersion ◽  
Phase Dispersion ◽  
Relative Standard ◽  
The Matrix ◽  
Low Volume ◽  
Nitrogen Phosphorus

Abstract A multiresidue method was developed for the determination of 12 organophosphorus insecticides (diazinon, parathion methyl, fenitrothion, pirimiphosmethyl, malathion, fenthion, chlorpyrifos, quinalphos, methidathion, ethion, azinphosmethyl, coumaphos), one carbamate (pirimicarb), and one amidine (amitraz) in unifloral and multifloral honeys. The analytical procedure was based on the matrix solid-phase dispersion of honey on a mixture of Florisil and anhydrous sodium sulfate in small glass columns and subsequent extraction with a low volume of hexane–ethyl acetate (90 + 10, v/v), assisted by sonication. The insecticide residues were determined by capillary chromatography with nitrogen–phosphorus detection and confirmed by mass spectrometry. Average recoveries at the 0.05–0.5 μg/g levels were >80% for organophosphorus insecticides and about 60% for the other insecticides, pirimicarb and amitraz, with relative standard deviations <10%. The detection limit for the different insecticides ranged between 6 and 15 μg/kg. The main advantages of the proposed method are that extraction and cleanup are performed in a single step with a low volume of organic solvent. The method is simple, rapid, and less laborious than conventional methods. Several Spanish honeys were analyzed with the proposed method and no residues of the studied insecticides were found.

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