Separation of 30S ribosomal proteins from sulfolobus acidocaldarius by ion exchange and gel permeation chromatography

1986 ◽  
Vol 22 (7-12) ◽  
pp. 432-432 ◽  
Author(s):  
J. R. Grün ◽  
R. -M. Kamp ◽  
R. Reinhardt
Keyword(s):  
Ion Exchange ◽  
Ribosomal Proteins ◽  
Gel Permeation Chromatography ◽  
Sulfolobus Acidocaldarius ◽  
Gel Permeation

scholarly journals Separation of a series of chromophores and fluorophores present in elastin

1975 ◽  
Vol 147 (2) ◽  
pp. 215-219 ◽  
Author(s):  
D P Thornhill
Keyword(s):  
Optical Properties ◽  
Ion Exchange ◽  
Fluorescence Spectra ◽  
Cation Exchanger ◽  
Gel Permeation Chromatography ◽  
Protein Molecule ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Intact Protein ◽  
Gel Permeation

Purified elastin was hydrolysed with HCl and manipulated under conditions that minimized oxidation. Gel-permeation chromatography on polyacrylamide gel and ion-exchange chromatography on dextran cation-exchanger each resulted in the separation of a series of yellow fluorescent fractions. These hitherto unreported ampholytes have fluorescence spectra that approximate to that of the intact protein, and account for its characteristic optical properties. Since the coloured fluorophores are confined to enzyme-resistant regions of the protein molecule they appear to have important structural implications.

Chitinases of Bacillus licheniformis B-6839: isolation and properties

1996 ◽  
Vol 42 (4) ◽  
pp. 307-315 ◽  
Author(s):  
Lesya A. Trachuk ◽  
Lyudmila P. Revina ◽  
Tatyana M. Shemyakina ◽  
Galina G. Chestukhina ◽  
Valentin M. Stepanov
Keyword(s):  
Ion Exchange ◽  
Bacillus Licheniformis ◽  
Gel Permeation Chromatography ◽  
The Other ◽  
Colloidal Chitin ◽  
Bacillus Circulans ◽  
Temperature Optimum ◽  
Chitinase A ◽  
Gel Permeation ◽  
Molecular Masses

Five chitinases were isolated from culture filtrates of Bacillus licheniformis B-6839 R and S variants by combination of hydrophobic, ion-exchange, and gel permeation chromatography. The enzymes had molecular masses of 66, 62, 53, 49, and 42 kDa. The chitinases revealed two activity optima against colloidal chitin at pH 4.5–5.5 and 9.0–9.5 and they were rather stable at pH 4.0–9.5. The temperature optimum of activity was 90 °C for the 62-kDa chitinase and 70 °C for the other enzymes. The 66-, 53-, and 42-kDa chitinases showed pronounced similarities in their N-terminal sequences and apparently belonged to the same group, which might be related to Bacillus circulans chitinase A1. The 49- and 62-kDa enzymes did not reveal structural similarities with other chitinases produced by the studied B. licheniformis strain. No relationship was found with the 89- and 76-kDa chitinases isolated earlier from B. licheniformis X-7u.Key words: Bacillus licheniformis, chitinase, multiplicity.

scholarly journals Retained folates in the rat

1977 ◽  
Vol 164 (3) ◽  
pp. 601-605 ◽  
Author(s):  
P A Barford ◽  
R J Staff ◽  
J A Blair
Keyword(s):  
Folic Acid ◽  
Ion Exchange ◽  
Gel Permeation Chromatography ◽  
Exchange Chromatography ◽  
Chromatographic Behaviour ◽  
Gel Permeation

The retention of radioactivity after doses of 14C- and 3H-labelled folic acid is described. Radioactivity was retained in liver, kidney and gut of rats for some time after administration of the dose. The retained radioactivity could not be displaced by large doses of unlabelled folic acid or unlabelled 5-methyltetrahydrofolate. 14C- and 3H-labbelled folates showed similar chromatographic behaviour onion-exchange chromatography to 5-methyltetrahydrofolate, and on ion-exchange and gel-permeation chromatography to synthetic pteroylhepta-gamma-glutamate.

scholarly journals Proteoglycans from pig aorta. Comparative study of their interactions with lipoproteins

1986 ◽  
Vol 235 (3) ◽  
pp. 823-831 ◽  
Author(s):  
J Wegrowski ◽  
M Moczar ◽  
L Robert
Keyword(s):  
Hyaluronic Acid ◽  
Ion Exchange ◽  
Chondroitin Sulphate ◽  
Gel Permeation Chromatography ◽  
Low Density Lipoproteins ◽  
Low Density ◽  
Hydrodynamic Size ◽  
Guanidinium Chloride ◽  
Protein Core ◽  
Gel Permeation

Different proteoglycans (PGs) were isolated from pig aorta for aggregation studies with hyaluronic acid and human low-density lipoproteins (LDL). Extraction of the intima-media with 4M-guanidinium chloride and digestion of the residue with collagenase solubilized 91% of aortic hexuronic acid content. From the guanidinium chloride extract two PGs were isolated by ion-exchange and gel-permeation chromatography: proteochondroitin sulphate (PGI) with a protein-core apparent Mr of 250 000 and proteodermatan-chondroitin sulphate (PGII) with a protein-core apparent Mr of 55 000. Only PGI forms high-Mr aggregates with hyaluronic acid. From the collagenase digest two other PGs were isolated: proteoheparan sulphate and proteochondroitin sulphate (PGIII and PGIV respectively). PGIV had a smaller hydrodynamic size than PGI. PGI and PGII formed insoluble complexes with human LDL in the presence of Ca2+. PGIII or PGIV did not form precipitates with the LDL. PGI and PGII, but neither PGIII nor PGIV, were bound to LDL-Sepharose. The main peaks of PGI and PGII were eluted from LDL-Sepharose with 60 mM- and 90 mM-NaCl respectively. The results indicate that aortic PGs have different interacting potentials with lipoproteins, depending on their Mr and their glycosaminoglycan composition.

Quantitative fractionation of casein mixtures by fast protein liquid chromatography

1987 ◽  
Vol 54 (3) ◽  
pp. 369-376 ◽  
Author(s):  
D. Thomas Davies ◽  
Andrew J. R. Law
Keyword(s):  
Liquid Chromatography ◽  
Ion Exchange ◽  
Deae Cellulose ◽  
Gel Permeation Chromatography ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Fast Protein Liquid Chromatography ◽  
Good Resolution ◽  
Gel Permeation

SummaryAlkylation of whole casein samples by reaction with cysteamine and cystamine in a bis-tris-propane–urea buffer (pH 7·0) followed by fast protein liquid chromatography (FPLC) at 20°C on a Mono Q HR5/5 column in the same buffer and using a NaCl gradient led to good resolution of the whole casein into fractions representing (i) γ2- plus γ3-caseins, (ii) κ-caseins, (iii) β-casein, (iv) αs2-caseins and (v) αsl-caseins, together with small amounts of unidentified materials. Quantitatively the FPLC values agreed well with those for αs1-, β-, αs2- and γ2- plus γ3-caseins obtained by ion-exchange chromatography on DEAE cellulose, Whatman DE52 and with those for º-caseins obtained by gel-permeation chromatography on Sephadex G–150.

Separation and Gel Permeation Analysis of natural Emulsion Stabilizers

1968 ◽  
Vol 8 (03) ◽  
pp. 253-259 ◽  
Author(s):  
C.A. Stout ◽  
S.W. Nicksic
Keyword(s):  
Molecular Weight ◽  
Ion Exchange ◽  
Crude Oil ◽  
Southern California ◽  
Average Molecular Weight ◽  
Gel Permeation Chromatography ◽  
Crude Oils ◽  
Ionic Character ◽  
Number Average Molecular Weight ◽  
Gel Permeation

Abstract The materials that stabilize emulsions of some Southern California crude oils were isolated from the produced crude. These substances were separated into acidic and nonionic fractions by ion exchange chromatography. Each fraction was further broken into groups of homogeneous molecular weight composition by gel permeation chromatography. Both ionic character and molecular weight of the individual fractions are reflected in the properties of the original crude oil emulsion. Specifically, the more acidic and the higher molecular weight fractions appear to be the most effective stabilizers. Introduction The treatment of oilfield emulsions is a continuing problem for the oil industry. In almost all cases, these are water-in-oil emulsions. Much more is known about the formation and stabilization of oil-in-water (O/W) emulsions than of the water-in-oil (W/O) type. To understand W/O emulsions, more information is needed on the materials responsible for their formation and stabilization. This is especially true in the case of oilfield emulsions where the stabilizing materials are complex mixtures of large asphaltene-like molecules. To study the chemical and physical properties of these emulsion stabilizers, it is necessary first to separate them from crude oil. This separation was achieved in the case of two Southern California crude oils. The materials that were isolated appear at first to be little different from an asphaltene mixture. However, if a mixture of distilled water and mineral oil containing about 1 percent of the natural emulsion stabilizers is passed through a colloid mill, the W/O emulsion formed may be stored in the laboratory for many months without any sign of breaking. This could not be done using undifferentiated asphaltenes from the same wells, indicating that there is a real difference between these materials and ordinary asphaltenes. The colloid mill used to generate the emulsion was required because the natural emulsion stabilizers had very little effect on oil-water interfacial tension. The one characteristic - complexity of composition - that emulsion stabilizers share with asphaltenes makes virtually impossible the exact structure determination of the components of the mixture. For this reason, it was decided to investigate first the influence of molecular weight on the ability of these materials to stabilize emulsions. The emulsion stabilizers were first separated into acidic and neutral fractions according to whether or not the material was retained on a weakly basic ion exchange column. The extraction procedure was not designed to remove basic components from the oils, and no attempt to isolate such a fraction was made. Following this separation selected fractions were analyzed by gel permeation chromatography. Gel permeation separates according to molecular size, largely independently of chemical or polar characteristics. A heterogeneous mixture is separated into a number of fractions having much more homogeneous molecular weight distribution. A number average molecular weight of each fraction is therefore close to the true molecular weight of the principal component of that fraction. The fraction weights, expressed as weight percent of the sample, and the number average molecular weight of a representative selection of the fractions, will be referred to in this paper as the molecular weight profile of the original sample. DESCRIPTION OF GEL PERMEATION ANALYSIS The first reported gel permeation analyses were for separating partially hydrolyzed protein molecules in an aqueous system using expanded starch granules. Most of the theoretical work on gel permeation has been done by biochemists. SPEJ P. 253ˆ

Investigations into the chemoattraction of the potato cyst nematodes Globodera rostochiensis and G. pallida towards fractionated potato root leachate

Nematology ◽  
2003 ◽  
Vol 5 (1) ◽  
pp. 65-75 ◽  
Author(s):  
Ken Devine ◽  
Peter Jones
Keyword(s):  
Ion Exchange ◽  
Gel Permeation Chromatography ◽  
Globodera Rostochiensis ◽  
Potato Cyst Nematodes ◽  
Cyst Nematodes ◽  
Potato Root ◽  
Second Stage ◽  
Gel Permeation ◽  
Common Fractions

AbstractThe behaviour of stimulated second stage juveniles (J2) (i.e., hatched in root leachate from potato cv. Cara) and unstimulated J2 (spontaneously hatched in water) of Globodera rostochiensis and G. pallida in response to fractionated and unfractionated potato root leachate (PRL) was investigated in attraction assays. In PRL, fractionated by combined ion-exchange-gel permeation chromatography on Sephadex G-10, three classes of semiochemicals with activity towards J2 were distinguished: i) chemoattractants; ii) chemostats, and iii) chemorepellents. The motility of PRL-hatched G. rostochiensis J2 in one fraction (12) at 10 days after their removal from the root leachate was significantly greater than that of water-hatched J2 apparently due to sensitisation of PRL-hatched J2. PRL-hatched J2 of G. pallida were attracted to different fractions than those of G. rostochiensis, whereas the water-hatched J2 from the two species were attracted to common fractions, indicating that sensitisation by exposure to PRL was species selective. The attraction of PRL-hatched PCN J2 to unfractionated PRL appeared to be dependent on the ratio of chemoattractant to chemorepellent semiochemicals in the leachate. For both species there was no detectable correlation between hatching activity and either attractiveness of root leachates from 12 potato genotypes or chemoattraction in PRL fractions, indicating that hatching factors were not active chemoattractants.

Sign in / Sign up

Export Citation Format

Share Document