RP-HPLC as an evaluation method for the behaviour of urinary trypsin inhibitors

1988 ◽  
Vol 26 (1) ◽  
pp. 369-371 ◽  
Author(s):  
G. Raspi ◽  
A. Lo Moro ◽  
M. Spinetti ◽  
M. Molinari
Keyword(s):  
Evaluation Method ◽  
Trypsin Inhibitors ◽  
Rp Hplc ◽  
Urinary Trypsin Inhibitors

Separation and quantitation of human plasma acid stable trypsin inhibitors by RP-HPLC

1989 ◽  
Vol 335 (4) ◽  
pp. 398-401
Author(s):  
Giorgio Raspi ◽  
Antonio Lo Moro ◽  
Maria Spinetti ◽  
Marica Molinari
Keyword(s):  
Human Plasma ◽  
Trypsin Inhibitors ◽  
Rp Hplc ◽  
Acid Stable

Immunological evaluation of urinary trypsin inhibitors in blood and urine: Role of N- & O-linked glycoproteins

2006 ◽  
Vol 24 (1) ◽  
pp. 5-15 ◽  
Author(s):  
Michael J. Pugia ◽  
Saeed A. Jortani ◽  
Manju Basu ◽  
Ronald Sommer ◽  
Hai-Hang Kuo ◽  
...  
Keyword(s):  
Trypsin Inhibitors ◽  
Immunological Evaluation ◽  
Urinary Trypsin Inhibitors

scholarly journals Characterization of Purified Urinary Trypsin Inhibitors

1977 ◽  
Author(s):  
A. Takada ◽  
Y. Takada ◽  
K. Minakata ◽  
H. Sumi
Keyword(s):  
Trypsin Inhibitor ◽  
Human Urine ◽  
Trypsin Inhibitors ◽  
High Concentration ◽  
No Inhibition ◽  
Urinary Trypsin Inhibitors ◽  
Inhibition Of Thrombin

Human urine was passed through Celite 545 column, and the non adsorbed eluate was adsorbed to Bentnite, then eluted with 2 % pyridine. The eluate was precipitated with ammonia sulfate, and the precipitate was dissolved, then passed through DEAE-Sephadex A-50, trypsin-Sepharose and Sephadex G-200. There were three types of urinary trypsin inhibitors (UTI) of m.w. of 69,000, 45,000, and 25,000 respectively. No difference in inhibitory spectra was observed among these three UTI’s. Purified UTI inhibited trypsin, chymotrypsin, and kallikrein. The inhibition of chymotrypsin did not exceed 50even at the high concentration of UTI. UTI inhibited plasmin to a lesser extent but no inhibition of thrombin, Cls, Clr or urokinase was observed.UTI had 7% carbohydrate and no HIS, PRO, or TRP was found in aminoacid analysis. UTI was not identical to α1A but related to inter α- trypsin inhibitor immunologically. Some persons excreted both UTI and α1A in the urine although most people did only UTI. Since α1A is not found in the urine of most people, UTI may not be the same as inter α-trypsin inhibitor which is filtered into the urine.

Urinary Trypsin Inhibitors Afford Cardioprotective Effects through Activation of PI3K-Akt and ERK Signal Transduction and Inhibition of p38 MAPK and JNK

Cardiology ◽  
2009 ◽  
Vol 114 (4) ◽  
pp. 264-270 ◽  
Author(s):  
Seok J. Kim ◽  
Kyung Y. Yoo ◽  
Cheol W. Jeong ◽  
Woong M. Kim ◽  
Hyung K. Lee ◽  
...  
Keyword(s):  
Signal Transduction ◽  
P38 Mapk ◽  
Trypsin Inhibitors ◽  
Cardioprotective Effects ◽  
Urinary Trypsin Inhibitors

Effect of Growth of Walker 256 Carcinosarcoma on the Fibrinolytic System of the Rat Plasma and Urine

1970 ◽  
Vol 56 (2) ◽  
pp. 71-81
Author(s):  
Gerda Mootse ◽  
Domenico Agostino ◽  
Eugene E. Cliffton
Keyword(s):  
Fibrinolytic Activity ◽  
Rat Plasma ◽  
Trypsin Inhibitors ◽  
Fibrinolytic System ◽  
Plasma Fibrinogen ◽  
Walker 256 Carcinosarcoma ◽  
Walker 256 ◽  
Significant Change ◽  
Urinary Trypsin Inhibitors

Growth of a transplanted Walker 256 carcinosarcoma in rats was accompanied by: 1) An increase in plasma fibrinogen and urinary trypsin inhibitors; 2) A significant decrease in plasma plasminogen and hematocrit; 3) A decrease in urokinase activity in urine; 4) A decrease in fibrinolytic activity of the euglobulin fraction of plasma and serum. There was no significant change in the plasma inhibitors for trypsin or bovine fibrinolysin.

Pathophysiology and diagnostic value of urinary trypsin inhibitors

2005 ◽  
Vol 43 (1) ◽  
pp. 1-16 ◽  
Author(s):  
Michael J. Pugia ◽  
John A. Lott
Keyword(s):  
Myocardial Infarction ◽  
Tissue Injury ◽  
Kidney Diseases ◽  
Acute Infection ◽  
Trypsin Inhibitors ◽  
Important Indicator ◽  
Inflammatory Agent ◽  
Markers Of Inflammation ◽  
Anti Inflammatory ◽  
Urinary Trypsin Inhibitors

AbstractInflammation is an important indicator of tissue injury. In the acute form, there is usually accumulation of fluids and plasma components in the affected tissues. Platelet activation and the appearance in blood of abnormally increased numbers of polymorphonucleocytes, lymphocytes, plasma cells and macrophages usually occur. Infectious disorders such as sepsis, meningitis, respiratory infection, urinary tract infection, viral infection, and bacterial infection usually induce an inflammatory response. Chronic inflammation is often associated with diabetes mellitus, acute myocardial infarction, coronary artery disease, kidney diseases, and certain auto-immune disorders, such as rheumatoid arthritis, organ failures and other disorders with an inflammatory component or etiology. The disorder may occur before inflammation is apparent. Markers of inflammation such as C-reactive protein (CRP) and urinary trypsin inhibitors have changed our appraisal of acute events such as myocardial infarction; the infarct may be a response to acute infection and (or) inflammation.We describe here the pathophysiology of an anti-inflammatory agent termed urinary trypsin inhibitor (uTi). It is an important anti-inflammatory substance that is present in urine, blood and all organs. We also describe the anti-inflammatory agent bikunin, a selective inhibitor of serine proteases. The latter are important in modulating inflammatory events and even shutting them down.

Evaluation method for imaging plate resolution by means of phase contrast transfer function

1995 ◽  
Vol 53 ◽  
pp. 662-663 ◽  
Author(s):  
T. Oikawa ◽  
H. Kosugi ◽  
F. Hosokawa ◽  
D. Shindo ◽  
M. Kersker
Keyword(s):  
Transfer Function ◽  
Phase Contrast ◽  
Evaluation Method ◽  
Amorphous Film ◽  
Imaging Plate ◽  
X Rays ◽  
Special Shape ◽  
Contrast Transfer Function ◽  
Contrast Image ◽  
Specimen Shape

Evaluation of the resolution of the Imaging Plate (IP) has been attempted by some methods. An evaluation method for IP resolution, which is not influenced by hard X-rays at higher accelerating voltages, was proposed previously by the present authors. This method, however, requires truoblesome experimental preperations partly because specially synthesized hematite was used as a specimen, and partly because a special shape of the specimen was used as a standard image. In this paper, a convenient evaluation method which is not infuenced by the specimen shape and image direction, is newly proposed. In this method, phase contrast images of thin amorphous film are used.Several diffraction rings are obtained by the Fourier transformation of a phase contrast image of thin amorphous film, taken at a large under focus. The rings show the spatial-frequency spectrum corresponding to the phase contrast transfer function (PCTF). The envelope function is obtained by connecting the peak intensities of the rings. The evelope function is offten used for evaluation of the instrument, because the function shows the performance of the electron microscope (EM).

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