Extraction chromatography of common anions in liquid-liquid anion exchange systems. Part. III. Monobasic aliphatic organic acids and their sodium salts as eluants

1979 ◽  
Vol 12 (9) ◽  
pp. 587-594 ◽  
Author(s):  
S. Przeszlakowski ◽  
R. Kocjan
Keyword(s):  
Organic Acids ◽  
Anion Exchange ◽  
Extraction Chromatography ◽  
Sodium Salts

Analysis of Organic Acids in Fruit Products by Anion Exchange Isolation and Gas Chromatographic Determination

1973 ◽  
Vol 56 (5) ◽  
pp. 1257-1263 ◽  
Author(s):  
David W Baker
Keyword(s):  
Organic Acids ◽  
Formic Acid ◽  
Anion Exchange ◽  
Exchange Resin ◽  
Trimethylsilyl Ether ◽  
Cation Exchange Resin ◽  
Chromatographic Determination ◽  
Alcoholic Extract ◽  
Resin Column ◽  
Acidic Components

Abstract The method described involves passing an alcoholic extract of fruit product through a cation exchange resin column and then through an anion exchange resin column. Neutral fruit components are washed from the columns with alcohol and acetone solutions. Acidic components trapped on the anion column are eluted using 6N formic acid in acetone. An aliquot of the eluate is evaporated to dryness after the addition of benzene to remove the excess formic acid as an azeotrope. The residue is allowed to react with bis(trimethylsilyl)acetamide or bis(trimethylsilyl)trifluoroacetamide to form trimethylsilyl ether-ester derivatives which are detected by gas chromatography on a nonpolar (OV-1) column. Good recoveries were obtained for some common organic acids (succinic, fumarie, malic, tartaric, trans- aconitic, and citric acids) from fruit juice.

Proflavine binding and release by sensitive and resistant Bacillus subtilis

1970 ◽  
Vol 16 (10) ◽  
pp. 973-981
Author(s):  
Gy. Barabas ◽  
B. M. Mehta ◽  
D. J. Kushner
Keyword(s):  
Escherichia Coli ◽  
Bacillus Subtilis ◽  
Organic Acids ◽  
Resistant Strain ◽  
Binding Capacity ◽  
Sensitive Strain ◽  
Growth Media ◽  
Sodium Salts ◽  
Salts Of Organic Acids ◽  
Resistant Cells

Proflavine binding of a sensitive strain of Bacillus subtilis and of a resistant strain derived from it was compared. Proflavine was bound very rapidly and more was bound at 0 °C than at 37 °C. Boiling increased the proflavine-binding capacity at 37 °C of sensitive but not of resistant cells. The binding capacity of sensitive and resistant cells suspended in buffer was the same; this was also true in various growth media. If cells were able to grow in the presence of proflavine their proflavine content decreased.Bound proflavine was released when cells were treated with growth media or with the salts of growth media. Sodium salts of organic acids also caused a release. This effect seemed due to their Na+ content, and was somewhat higher for resistant than for sensitive cells. The mechanism of proflavine resistance in B. subtilis is probably different from that of Escherichia coli, which is thought to depend on an energy-driven release of bound proflavine.

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