Characterization of centromere arrangements and test for random distribution in G0, G1, S, G2, G1, and early S′ phase in human lymphocytes

1992 ◽  
Vol 88 (6) ◽  
pp. 673-682 ◽  
Author(s):  
R. Weimer ◽  
T. Haaf ◽  
J. Krüger ◽  
M. Poot ◽  
M. Schmid
Keyword(s):  
Random Distribution ◽  
Human Lymphocytes ◽  
S Phase

Characterization of S-Phase Specific BRCA1-Containing Complex

2002 ◽  
Author(s):  
Natsuko Chiba ◽  
Jeffrey D. Parvin
Keyword(s):  
S Phase

Characterization of Different Deoxyribonucleases in Human Lymphocytes

1975 ◽  
Vol 30 (11-12) ◽  
pp. 781-784 ◽  
Author(s):  
E. Jürgen Zöllner ◽  
Hans Störger ◽  
Hans-Joachim Breter ◽  
Rudolf Zahn
Keyword(s):  
Human Lymphocytes ◽  
Disc Electrophoresis ◽  
The Other ◽  
Lower Activity ◽  
Nuclear Fraction ◽  
Polyacrylamide Gels ◽  
Cytoplasmic Fraction ◽  
Acid Deoxyribonuclease ◽  
Deoxyribonuclease Activity

Abstract Deoxyribonucleases, Disc Electrophoresis, Lymphocytes Four groups of deoxyribonuclease activities from human lymphocytes have been characterized by deoxyribonuclease assay in DNA-containing polyacrylamide gels following their separation by disc-electrophoresis. All activities hydrolyse DNA endonucleolytically. One neutral deoxyribo­ nuclease found in the cytoplasmic fraction prefers native or UV-irradiated DNA over denatured DNA as substrate and is a 5′-monoester former. Two groups of acid deoxyribonuclease activities are detectable in the nuclear fraction. Both are 3′-monoester formers. One is as well active with denatured DNA as with native DNA, the other one shows the same activity with native and UV-irradiated DNA but lower activity with denatured DNA. An alkaline deoxyribonuclease activity, also localized in the nucleus, is a 5′ -monoester DNA as substrate.

Diversity metrics, species turnovers and nestedness of bird assemblages in a deep karst sinkhole

2017 ◽  
Vol 63 (2) ◽  
pp. 8-16 ◽  
Author(s):  
Corrado Battisti ◽  
Marco Giardini ◽  
Francesca Marini ◽  
Lorena Di Rocco ◽  
Giuseppe Dodaro ◽  
...  
Keyword(s):  
Species Richness ◽  
Random Distribution ◽  
Diversity Index ◽  
Species Turnover ◽  
Floristic Composition ◽  
Bird Assemblages ◽  
Β Diversity ◽  
Stick Model ◽  
Karst Sinkhole

We reported a study on breeding birds occurring inside an 80 m-deep karst sinkhole, with the characterization of the assemblages recorded along its semi-vertical slopes from the upper edge until the bottom. The internal sides of the sinkhole have been vertically subdivided in four belts about 20 m high. The highest belt (at the upper edge of the cenote) showed the highest values in mean number of bird detections, mean and normalized species richness, and Shannon diversity index. The averaged values of number of detections and species richness significantly differ among belts. Species turnover (Cody’s β-diversity) was maximum between the highest belts. Whittaker plots showed a marked difference among assemblages shaping from broken-stick model to geometric series, and explicited a spatial progressive stress with a disruption in evenness towards the deepest belts. Bird assemblages evidenced a nested subset structure with deeper belts containing successive subsets of the species occurring in the upper belts. We hypothesize that, at least during the daytime in breeding season, the observed non-random distribution of species along the vertical stratification is likely due to (i) the progressive simplification both of the floristic composition and vegetation structure, and (ii) the paucity of sunlight as resources from the upper edge to the inner side of the cenote.

scholarly journals Characterization of the Pre-meiotic S Phase through Incorporation of BrdU during Spermatogenesis in the Rat

2013 ◽  
Vol 61 (9) ◽  
pp. 680-689 ◽  
Author(s):  
Israel Muñoz-Velasco ◽  
Rosario Ortíz ◽  
Olga M. Echeverría ◽  
María L. Escobar ◽  
Gerardo H. Vázquez-Nin
Keyword(s):  
S Phase

scholarly journals Molecular and epidemiological characterization of recurrent Mycobacterium ulcerans infections in Benin

2021 ◽  
Vol 15 (12) ◽  
pp. e0010053
Author(s):  
Ronald Gnimavo ◽  
Alban Besnard ◽  
Horace Degnonvi ◽  
Juliana Pipoli Da Fonseca ◽  
Marie Kempf ◽  
...  
Keyword(s):  
Random Distribution ◽  
Buruli Ulcer ◽  
Mycobacterium Ulcerans ◽  
Neglected Tropical Disease ◽  
Contaminated Water ◽  
Ulcer Recurrence ◽  
Paradoxical Reaction ◽  
Environmental Mycobacterium ◽  
Epidemiological Characterization

Background Buruli ulcer is a neglected tropical disease caused by Mycobacterium ulcerans, an environmental mycobacterium. Although transmission of M. ulcerans remains poorly understood, the main identified risk factor for acquiring Buruli ulcer is living in proximity of potentially contaminated water sources. Knowledge about the clinical features of Buruli ulcer and its physiopathology is increasing, but little is known about recurrence due to reinfection. Methodology/Principal findings We describe two patients with Buruli ulcer recurrence due to reinfection with M. ulcerans, as demonstrated by comparisons of DNA from the strains isolated at the time of the first diagnosis and at recurrence. Based on the spatial distribution of M. ulcerans genotypes in this region and a detailed study of the behavior of these two patients with respect to sources of water as well as water bodies and streams, we formulated hypotheses concerning the sites at which they may have been contaminated. Conclusions/Significance Second episodes of Buruli ulcer may occur through reinfection, relapse or a paradoxical reaction. We formally demonstrated that the recurrence in these two patients was due to reinfection. Based on the sites at which the patients reported engaging in activities relating to water, we were able to identify possible sites of contamination. Our findings indicate that the non-random distribution of M. ulcerans genotypes in this region may provide useful information about activities at risk.

scholarly journals Flow Cytometric Characterization of S-phase Fraction and Ploidy in Lymph Node Aspirates from Dogs with Lymphoma

2018 ◽  
Vol 161 ◽  
pp. 34-42 ◽  
Author(s):  
B. Miniscalco ◽  
A. Poggi ◽  
V. Martini ◽  
E. Morello ◽  
M. Sulce ◽  
...  
Keyword(s):  
Lymph Node ◽  
S Phase ◽  
Phase Fraction ◽  
Flow Cytometric

Application of the Solution of the Inverse Light Scattering Problem for Characterization of Mononuclear Cells

2009 ◽  
Vol 4 (2) ◽  
pp. 61-68
Author(s):  
Dmitriy Strokotov ◽  
Yuriy Pichugin ◽  
Maxim Yurkin ◽  
Mariya Gridina ◽  
Oleg Serov ◽  
...  
Keyword(s):  
Stem Cells ◽  
Light Scattering ◽  
Mononuclear Cells ◽  
Human Lymphocytes ◽  
Scattering Problem ◽  
Refractive Indices ◽  
Single Particles ◽  
Differentiation Status

In this manuscript we propose two methods to solve inverse light scattering problem for single particles, which can be described as a coated sphere. The efficiency of the methods is illustrated by characterization of lymphocytes and stem cells using light scattering patterns obtained with scanning flow cytometer. Both methods, spectral and global optimization, were used to obtain diameters and refractive indices of the cytoplasm and the nucleus of mice embryo stem cells and human lymphocytes. These results agree with data obtained from other studies. Determination of these parameters is important for diagnostics of pathological states of lymphocytes and differentiation status of embryo stem cells. Moreover, methods described in this manuscript are applicable to all mononuclear cells. We also considered limitations of these methods and their possible improvements.

Expression of A2B adenosine receptors in human lymphocytes: their role in T cell activation

1999 ◽  
Vol 112 (4) ◽  
pp. 491-502
Author(s):  
M. Mirabet ◽  
C. Herrera ◽  
O.J. Cordero ◽  
J. Mallol ◽  
C. Lluis ◽  
...  
Keyword(s):  
T Cell ◽  
T Cell Activation ◽  
Cell Activation ◽  
Human Peripheral Blood ◽  
Human Lymphocytes ◽  
Interleukin 2 ◽  
Cell Receptor ◽  
Physiological Characterization ◽  
A2a Receptors

Extracellular adenosine has a key role in the development and function of the cells of the immune system. Many of the adenosine actions seem to be mediated by specific surface receptors positively coupled to adenylate cyclase: A2A and A2B. Despite the fact that A2A receptors (A2ARs) can be easily studied due to the availability of the specific agonist CGS21680, a pharmacological and physiological characterization of adenosine A2B receptors (A2BRs) in lymphocytes has not been possible due to the lack of suitable reagents. Here we report the generation and characterization of a polyclonal antipeptide antibody raised against the third extracellular loop of the A2BR human clone which is useful for immunocytochemical studies. This antibody has permitted the detection of A2BR+ cells in lymphocyte samples isolated from human peripheral blood. The pharmacology of cAMP-producing compounds is consistent with the presence of functional A2BRs but not of A2A receptors in these human cells. The percentage of A2BR-expressing cells was similar in the CD4(+) or CD8(+) T cell subpopulations. Interestingly activation signals delivered by either phytohemagglutinin or anti-T cell receptor/CD3 complex antibodies led to a significant increase in both the percentage of cells expressing the receptor and the intensity of the labeling. These receptors are functional since interleukin-2 production in these cells is reduced by NECA but not by R-PIA or CGS21680. These results show that A2BR expression is regulated in T cell activation and suggest that the role of adenosine in lymphocyte deactivation is mediated by A2BRs.

scholarly journals Inhibition of human lymphocyte proliferation by monoclonal antibody to transferrin receptor

Blood ◽  
1983 ◽  
Vol 62 (4) ◽  
pp. 821-826 ◽  
Author(s):  
J Mendelsohn ◽  
I Trowbridge ◽  
J Castagnola
Keyword(s):  
Cell Cycle ◽  
Monoclonal Antibody ◽  
Lymphocyte Proliferation ◽  
Human Lymphocytes ◽  
Nitrilotriacetic Acid ◽  
S Phase ◽  
Inhibitory Effects ◽  
Mitogenic Response ◽  
Cell Cycle Inhibition ◽  
Partial Reversal

Abstract A monoclonal antibody, 42/6, which blocks the binding of transferrin to its receptor on the cell membrane, inhibits proliferation of human lymphocytes stimulated by phytohemagglutinin. Anti-receptor antibody B3/25, which does not block transferrin binding, does not alter the mitogenic response. Addition of soluble iron, in the form of ferric nitrilotriacetic acid, results in partial reversal of inhibition. Lymphocytes in the quiescent phase of the cell cycle at the time of 42/6 antibody addition are unable to traverse S phase, whereas cells actively proliferating when antibody is added are sensitive to its inhibitory effects throughout all phases of the cell cycle. Inhibition is static rather than cidal, since it can be reversed by removal of antibody after up to 48 hr of exposure.

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