Determination of thiamine and thiamine phosphates as thiochrome derivatives by reversed-phase chromatography on polystyrene packing materials

1984 ◽  
Vol 18 (8) ◽  
pp. 424-426 ◽  
Author(s):  
J. Bontemps ◽  
L. Bettendorff ◽  
J. Lombet ◽  
G. Dandrifosse ◽  
E. Schoffeniels ◽  
...  
Keyword(s):  
Reversed Phase ◽  
Reversed Phase Chromatography ◽  
Packing Materials ◽  
Thiamine Phosphates

Determination of isotope fractionation of Cr(iii) during oxidation by LC/low-resolution MC-ICPMS

2020 ◽  
Vol 35 (3) ◽  
pp. 560-566
Author(s):  
Jakub Karasiński ◽  
Cuc Thi Nguyen-Marcińczyk ◽  
Marcin Wojciechowski ◽  
Ewa Bulska ◽  
Ludwik Halicz
Keyword(s):  
Inductively Coupled Plasma ◽  
Isotope Fractionation ◽  
Isotopic Analysis ◽  
Reversed Phase ◽  
Ion Pair ◽  
Reversed Phase Chromatography ◽  
Inductively Coupled ◽  
Plasma Mass Spectrometry ◽  
Sensitive Method

A robust and sensitive method for the precise isotopic analysis of 53Cr/50Cr during Cr(iii) oxidation by coupling ion pair reversed-phase chromatography and Multicollector Inductively Coupled Plasma Mass Spectrometry (MC-ICPMS) is presented.

scholarly journals Determination of DiazaCon in Quail Feed and Quail Serum by Ion Pair Reversed-Phase Chromatography

2001 ◽  
Vol 84 (3) ◽  
pp. 634-639 ◽  
Author(s):  
John J Johnston ◽  
Margaret J Goodall ◽  
Jerome C Hurley ◽  
Christi A Yoder ◽  
Lowell A Miller
Keyword(s):  
Aqueous Solution ◽  
Ion Pairing ◽  
Reversed Phase ◽  
Ion Pair ◽  
Butyl Chloride ◽  
Reversed Phase Chromatography ◽  
Limits Of Detection ◽  
The Mean ◽  
Liquid Chromatographic

Abstract Liquid chromatographic (LC) methods were developed for quantitating the potential avian contraceptive DiazaCon in quail feed and serum. DiazaCon was extracted from ground quail feed with basic n-butyl chloride. The n-butyl chloride extract was evaporated to dryness. The DiazaCon residues were dissolved in an aqueous methanolic ion pairing solution and quantitated by LC at 206 nm. Avian sera was combined with an equal volume of a pH 4 aqueous solution of ion pairing reagent and filtered to remove interfering proteins. DiazaCon was quantitated by LC. Mean recoveries for 500 and 2000 ppm fortified feed were 89.1 and 91.0%, respectively. The mean recovery for sera fortified at 5 levels ranging from 35 to 2000 ppm was 84.9%. Method limits of detection were approximately 14 and 13 ppm for feed and sera, respectively.

scholarly journals Chromatographic Methods for the Determination of Primula Acid 1 Content in Primulae extractum fluidum

2020 ◽  
Keyword(s):  
Calibration Curve ◽  
Mobile Phase ◽  
Active Component ◽  
Reversed Phase ◽  
Hplc Method ◽  
Reversed Phase Chromatography ◽  
Triterpenoid Saponins ◽  
Simple Preparation ◽  
Primula Veris

Primula veris L. (Primulaceae)is healing plant, whose root is officially used to treat cough associated with cold. Other reported indications are respiratory, thoracic and mediastinal disorders. These effects are result of high contents of triterpenoid saponins and phenolic glycosides. Primula acid 1 (PA 1, also primulasaponin 1) is main active component in Primula elatior L. This paper presents an optimized high pressure liquid chromatography (HPLC) method for the determination of primula acid 1 content in Primulae extractum fluidum. TLC was performed to check for the presence of the substance of interest. The determination was performed by reversed phase chromatography using C18 as a stationary phase. The mobile phase used for separation consisted of 0.2% H3PO4 and acetonitrile. This method was validated through different parameters. The detection limit for primula acid 1 was LD=10.41 µg/ml, and the quantification limitwas LQ=34.69 µg/ml. In order to determine the content of primula acid 1, a calibration curve was constructed, and the content of primula acid 1 was calculated by the equation of the calibration curve and was 0.2793 mg per gram of extract. The results and simple preparation of sample show that HPLC is the method of choice for this type of analysis.

Determination of Pyridoxine in Dietary Supplements by Liquid Chromatography with UV, Fluorescence, and Mass Spectrometric Detection: Single-Laboratory Validation

2013 ◽  
Vol 96 (2) ◽  
pp. 265-275 ◽  
Author(s):  
Robert J Goldschmidt ◽  
Wayne R Wolf
Keyword(s):  
Dietary Supplements ◽  
Vitamin B6 ◽  
Internal Standard ◽  
Reversed Phase ◽  
Reversed Phase Chromatography ◽  
Uv Fluorescence ◽  
Ms Detection ◽  
Different Types ◽  
Single Laboratory

Abstract A single-laboratory validation was performed for a method that determines pyridoxine, one of the B6 vitamers, in dietary supplements using LC and UV, fluorescence, or MS detection. The method was adapted for use with either HPLC or ultra-performance LC (UPLC). Pyridoxine is extracted from samples using 0.1 M formic acid, and specific conditions are adjusted for each of the different types of supplement materials examined. Reversed-phase chromatography with C18-based columns is used in both HPLC and UPLC. Fluorescence detection, often used in chromatographic analyses of vitamin B6 in foods, was successfully used here, but offered no great advantages over UV detection in the supplement materials tested. MS detection was also satisfactory, although use of an internal standard was required. Accuracy of the method was demonstrated in several ways, including use of a standard reference material. Precision and repeatability of the method were found acceptable by analysis of variance and HorRat repeatability calculations.

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