Determination of the micelle-solute association constants of some benzene and naphthalene derivatives by micellar high performance liquid chromatograph

1989 ◽  
Vol 28 (7-8) ◽  
pp. 379-384 ◽  
Author(s):  
M. L. Marina ◽  
S. Vera ◽  
A. R. Rodriguez
Keyword(s):  
High Performance ◽  
Association Constants ◽  
High Performance Liquid Chromatograph ◽  
Naphthalene Derivatives ◽  
Liquid Chromatograph ◽  
Benzene And Naphthalene Derivatives

Efficient high performance liquid chromatograph/ultraviolet method for determination of diclofenac and 4′-hydroxydiclofenac in rat serum

2006 ◽  
Vol 830 (2) ◽  
pp. 231-237 ◽  
Author(s):  
Lata Kaphalia ◽  
Bhupendra S. Kaphalia ◽  
Santosh Kumar ◽  
Mary F. Kanz ◽  
Mary Treinen-Moslen
Keyword(s):  
High Performance ◽  
High Performance Liquid Chromatograph ◽  
Rat Serum ◽  
Liquid Chromatograph

Determination of thiamin and thiamin phosphate esters in blood by liquid chromatography with post-column derivatization.

1983 ◽  
Vol 29 (12) ◽  
pp. 2073-2075 ◽  
Author(s):  
M Kimura ◽  
Y Itokawa
Keyword(s):  
High Performance ◽  
Sodium Hydroxide Solution ◽  
Potassium Ferricyanide ◽  
Phosphate Esters ◽  
High Performance Liquid Chromatograph ◽  
Liquid Chromatograph ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic ◽  
Thiamin Pyrophosphate

Abstract We demonstrate a liquid-chromatographic method involving post-column derivatization for determining the concentration of thiamin and its phosphate esters in human blood. Blood, erythrocytes, or plasma is deproteinized and centrifuged. Aliquots of the samples are applied to a mu Bondapak C18 column attached to a "high-performance" liquid chromatograph. Addition of potassium ferricyanide/sodium hydroxide solution to the column effluent with a proportioning pump converts thiamin phosphates into fluorophores, the intensities of which are measured with a spectrofluorophotometer. Thiamin, thiamin monophosphate, thiamin pyrophosphate, and thiamin triphosphate eluted as single peaks; no coeluting substances were detected. Thiamin pyrophosphate was the ester present in greatest concentration, followed by thiamin triphosphate; thiamin monophosphate and thiamin were present in slight amounts. This method allows easy determination of thiamin and its phosphate esters in 0.1 mL of blood.

scholarly journals Simultaneous Determination of Four Marker Compounds in Lobelia chinensis Lour. Extract by HPLC-PDA

2021 ◽  
Vol 11 (24) ◽  
pp. 12080
Author(s):  
Beom-Geun Jo ◽  
Young-Hun Park ◽  
Ki Hyun Kim ◽  
Su-Nam Kim ◽  
Min Hye Yang
Keyword(s):  
Formic Acid ◽  
High Performance ◽  
Reverse Phase ◽  
High Performance Liquid Chromatograph ◽  
Liquid Chromatograph ◽  
Snake Bites ◽  
Antiviral Activities ◽  
Photodiode Array ◽  
Marker Compounds

Lobelia chinensis Lour. (L. chinensis) has traditionally been used as a treatment for snake bites, high fever, jaundice, edema, and diarrhea, and modern studies have reported its anti-inflammatory, antioxidant, and antiviral activities. L. chinensis contains various compounds, such as flavonoids and coumarins, and its flavonoid components have been identified in many studies. In this study, a high-performance liquid chromatograph equipped with a photodiode array (PDA) detector and an Aegispak C18-L reverse-phase column (4.6 mm × 250 mm i.d., 5 μm) was used to simultaneously analyze four marker components in L. chinensis for standardization purposes. HPLC-PDA (detection at 340 nm), performed using a 0.1% formic acid-water/0.1% formic acid-acetonitrile gradient, separated the four marker compounds: luteolin-7-O-β-d-glucuronopyranosyl (1→2)-O-β-d-glucuronopyranoside, clerodendrin, chrysoeriol-7-O-diglucuronide, and diosmin. The developed analytical method showed excellent linearity values (r2 > 0.9991), limits of detection (LODs: 0.376–2.152 μg/mL), limits of quantification (LOQs: 1.147–6.521 μg/mL), intra- and inter-day precisions (RSD < 1.96%), and analyte recoveries (96.83–127.07%; RSD < 1.73%); thus, it was found to be suitable for the simultaneous analysis of these four marker compounds in L. chinensis.

Determination of Coumaphos and Its Oxygen Analog in Eggs and Milk by Using a Multiresidue Method with Liquid Chromatographic Quantitation and Capillary Gas Chromatographic/Mass Spectrometric Confirmation

1983 ◽  
Vol 66 (6) ◽  
pp. 1353-1357
Author(s):  
Richard T Krause ◽  
Zhao Min ◽  
Sharona H Shotkin
Keyword(s):  
High Performance ◽  
Mass Spectrometric ◽  
Fluorescence Detector ◽  
High Performance Liquid Chromatograph ◽  
Liquid Chromatograph ◽  
Multiresidue Method ◽  
Oxygen Analog ◽  
Liquid Chromatographic ◽  
Chromatographic Mass

Abstract A multiresidue method for carbamate insecticides was adapted for the determination of coumaphos and its oxygen analog in eggs and milk. Eggs were extracted with acetonitrile and milk was extracted with acetone. Co-extractives were removed using liquid partitioning and charcoal column procedures described in the carbamate method. Coumaphos and its oxygen analog were determined by using a high performance liquid chromatograph equipped with a fluorescence detector. Recovery studies were performed for the 2 compounds at levels of 0.01 and 0.10 ppm in eggs and 0.01 and 0.02 ppm in milk. Overall average recovery was 100% (range 95-109%). In a trial of the method by another laboratory, the recovery of coumaphos and its oxygen analog from milk averaged 87 and 96%, respectively. Data are presented on the capillary gas chromatographic/mass spectrometric confirmation of coumaphos residues.

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