Pore formation by the sea anemone cytolysin equinatoxin II in red blood cells and model lipid membranes

1993 ◽  
Vol 131 (1) ◽  
pp. 11-22 ◽  
Author(s):  
Giovanna Belmonte ◽  
Cecilia Pederzolli ◽  
Peter Maček ◽  
Gianfranco Menestrina
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Lipid Membranes ◽  
Pore Formation ◽  
Sea Anemone ◽  
Model Lipid Membranes ◽  
Equinatoxin Ii

Corrigendum to “Stycholysin II, a cytolysin from the sea anemone Stichodactyla helianthus promotes higher hemolysis in aged red blood cells” [Toxicon 51 (2008) 1383–1390]

Toxicon ◽  
2008 ◽  
Vol 52 (1) ◽  
pp. 194
Author(s):  
Gloria Celedón ◽  
Gustavo González ◽  
Daniela Barrientos ◽  
Jose Pino ◽  
Fabiola Venegas ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Sea Anemone

Stycholysin II, a cytolysin from the sea anemone Stichodactyla helianthus promotes higher hemolysis in aged red blood cells

Toxicon ◽  
2008 ◽  
Vol 51 (8) ◽  
pp. 1383-1390 ◽  
Author(s):  
Gloria Celedón ◽  
Gustavo González ◽  
Daniela Barrientos ◽  
Jose Pino ◽  
Fabiola Venegas ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Sea Anemone

scholarly journals The Lipid-anchored Ectodomain of Influenza Virus Hemagglutinin (GPI-HA) Is Capable of Inducing Nonenlarging Fusion Pores

2000 ◽  
Vol 11 (4) ◽  
pp. 1143-1152 ◽  
Author(s):  
Ruben M. Markosyan ◽  
Fredric S. Cohen ◽  
Grigory B. Melikyan
Keyword(s):  
Influenza Virus ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Pore Formation ◽  
Transmembrane Domain ◽  
Fusion Pore ◽  
Influenza Virus Hemagglutinin ◽  
Initial Membrane ◽  
Optimal Fusion ◽  
Pass Through

GPI-linked hemagglutinin (GPI-HA) of influenza virus was thought to induce hemifusion without pore formation. Cells expressing either HA or GPI-HA were bound to red blood cells, and their fusion was compared by patch-clamp capacitance measurements and fluorescence microscopy. It is now shown that under more optimal fusion conditions than have been used previously, GPI-HA is also able to induce fusion pore formation before lipid dye spread, although with fewer pores formed than those induced by HA. The GPI-HA pores did not enlarge substantially, as determined by the inability of a small aqueous dye to pass through them. The presence of 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate or octadecylrhodamine B in red blood cells significantly increased the probability of pore formation by GPI-HA; the dyes affected pore formation to a much lesser degree for HA. This greater sensitivity of pore formation to lipid composition suggests that lipids are a more abundant component of a GPI-HA fusion pore than of an HA pore. The finding that GPI-HA can induce pores indicates that the ectodomain of HA is responsible for all steps up to the initial membrane merger and that the transmembrane domain, although not absolutely required, ensures reliable pore formation and is essential for pore growth. GPI-HA is the minimal unit identified to date that supports fusion to the point of pore formation.

scholarly journals ЗМІНИ ВЛАСТИВОСТЕЙ ЕРИТРОЦИТІВ У СОБАК

2016 ◽  
Vol 18 (3(71)) ◽  
pp. 3-6
Author(s):  
M.V. Anforova ◽  
V.I. Golovakha ◽  
O.V. Piddubnyak ◽  
M.J. Tyshkivskyy
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Lipid Membranes ◽  
Acid Resistance ◽  
Small Animals ◽  
Hemoglobin Content ◽  
Population Composition ◽  
Rapid Destruction ◽  
Healthy Dogs ◽  
Composition Changes

One of the most important indicators is the assessment of erythroсytopoezis is acid resistance of erythrocytes and their population composition. Changes in these parameters are the most studied in humans, cattle and horses. In small animals, including dogs, the issue is not highlighted enough. Therefore, the study of changes of erytroсytopoezis by using these tests is important. The study erythroсytopoezis parameters was performed in dogs of all ages (1.5–year, 3 – 6 years and 7 and older years of age). It was found that in clinically healthy dogs generall erythroсytopoezis indicators (total number of red blood cells, hemoglobin content, hematocrit value, the index «red» blood – МСН and МСV), regardless of age, did not significantly change. Yet in dogs older than 7 years there were found some changes on the the part of erythroсytopoezis. This was pointed by the changes of the population composition of red blood cells and their acid resistance. In particular, the animals showed an increased number of «old» populations of red blood cells and some changes of their hemolytic resistance. Particularly, the height of the erytrohram peak was 15% higher than in younger animals. The very peak was sharp, indicating the rapid destruction of the «young» red cells and degeneration of their lipid membranes. 

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