Tetracycline-regulated gene expression following direct gene transfer into mouse skeletal muscle

1995 ◽  
Vol 21 (4) ◽  
pp. 233-240 ◽  
Author(s):  
Jyotsna Dhawan ◽  
Thomas A. Rando ◽  
Sarah L. Elson ◽  
Hermann Bujard ◽  
Helen M. Blau
Keyword(s):  
Gene Expression ◽  
Skeletal Muscle ◽  
Gene Transfer ◽  
Direct Gene Transfer ◽  
Mouse Skeletal Muscle ◽  
Direct Gene ◽  
Regulated Gene Expression

Plasmid DNA Is Superior to Viral Vectors for Direct Gene Transfer into Adult Mouse Skeletal Muscle

1993 ◽  
Vol 4 (6) ◽  
pp. 733-740 ◽  
Author(s):  
Heather L. Davis ◽  
Barbara A. Demeneix ◽  
Béatrice Quantin ◽  
Josée Coulombe ◽  
Robert G. Whalen
Keyword(s):  
Skeletal Muscle ◽  
Gene Transfer ◽  
Plasmid Dna ◽  
Viral Vectors ◽  
Adult Mouse ◽  
Direct Gene Transfer ◽  
Mouse Skeletal Muscle ◽  
Direct Gene

Direct gene transfer in skeletal muscle: plasmid DNA-based immunization against the hepatitis B virus surface antigen

Vaccine ◽  
1994 ◽  
Vol 12 (16) ◽  
pp. 1503-1509 ◽  
Author(s):  
Heather L Davis ◽  
Marie-Louise Michel ◽  
Maryline Mancini ◽  
Martin Schleef ◽  
Robert G Whalen
Keyword(s):  
Skeletal Muscle ◽  
Hepatitis B Virus ◽  
Gene Transfer ◽  
Hepatitis B ◽  
Surface Antigen ◽  
Plasmid Dna ◽  
Direct Gene Transfer ◽  
Virus Surface ◽  
B Virus ◽  
Direct Gene

Analysis of recombinant gene expression and function in arteries following direct gene transfer

1994 ◽  
Vol 109 (1-2) ◽  
pp. 336
Author(s):  
E.G. Nabel ◽  
D. Gordon ◽  
R. Derynck ◽  
C. Haudenschild ◽  
T. Maciag ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Transfer ◽  
Direct Gene Transfer ◽  
Recombinant Gene Expression ◽  
Recombinant Gene ◽  
Direct Gene ◽  
And Function

Skeletal muscle gene transfer: regeneration-associated deregulation of fast troponin I fiber type specificity

2000 ◽  
Vol 278 (6) ◽  
pp. C1266-C1274 ◽  
Author(s):  
Patricia L. Hallauer ◽  
George Karpati ◽  
Kenneth E. M. Hastings
Keyword(s):  
Skeletal Muscle ◽  
Gene Transfer ◽  
Plasmid Dna ◽  
Troponin I ◽  
Recombinant Adenovirus ◽  
Fiber Type ◽  
Direct Gene Transfer ◽  
Adult Muscle ◽  
Direct Gene ◽  
Muscle Gene

Direct gene transfer into skeletal muscle in vivo presents a convenient experimental approach for studies of adult muscle gene regulatory mechanisms, including fast vs. slow fiber type specificity. Previous studies have reported preferential expression of fast myosin heavy chain and slow myosin light chain and troponin I (TnIslow) gene constructs in muscles enriched in the appropriate fiber type. We now report a troponin I fast (TnIfast) direct gene transfer study. We injected into the mouse soleus muscle plasmid DNA or recombinant adenovirus carrying a TnIfast/ β-galactosidase (β-gal) reporter construct that had previously been shown to be expressed specifically in fast fibers in transgenic mice. Surprisingly, microscopic histochemical analysis 1 and 4 wk postinjection showed similar TnIfast/β-gal expression in fast and slow fibers. A low but significant level of muscle fiber segmental regeneration was evident in muscles 1 wk postinjection, and TnIfast/β-gal expression was preferentially targeted to regenerating fiber segments. This finding can explain why TnIfast constructs are deregulated with regard to fiber type specificity, whereas the myosin constructs previously studied are not. The involvement of regenerating fiber segments in transduction by plasmid DNA and recombinant adenoviruses injected into intact normal adult muscle is an unanticipated factor that should be taken into account in the planning and interpretation of direct gene transfer experiments.

Polyethylene glycol-mediated direct gene transfer in Nicotiana spp.

1992 ◽  
Vol 85 (2) ◽  
pp. 345-351 ◽  
Author(s):  
Gokarna B. Gharti-Chhetri ◽  
Wichai Cherdshewasart ◽  
Jocelyne Dewulf ◽  
Michel Jacobs ◽  
loan Negrutiu
Keyword(s):  
Polyethylene Glycol ◽  
Gene Transfer ◽  
Direct Gene Transfer ◽  
Direct Gene
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