Characterization of the regulatory region ofAdra2c, the gene encoding the murine a2C adrenoceptor subtype

2004 ◽  
Vol 11 (6) ◽  
pp. 886-901
Author(s):  
Wen-Kwei Chen ◽  
Nan-Chi A. Chang ◽  
Yen-Hwa Chang ◽  
Kuo-Long Chang ◽  
Shinn-Chih Wu ◽  
...  
Keyword(s):  
Regulatory Region ◽  
Gene Encoding

Characterization of the Regulatory Region of Adra2c, the Gene Encoding the Murine α2C Adrenoceptor Subtype

2004 ◽  
Vol 11 (6) ◽  
pp. 886-901 ◽  
Author(s):  
Wen-Kwei Chen ◽  
Nan-Chi A. Chang ◽  
Yen-Hwa Chang ◽  
Kuo-Long Chang ◽  
Shinn-Chih Wu ◽  
...  
Keyword(s):  
Regulatory Region ◽  
Gene Encoding

Structural and functional characterization of the upstream regulatory region of the human gene encoding prostate apoptosis response factor-4

Gene ◽  
2002 ◽  
Vol 295 (1) ◽  
pp. 109-116 ◽  
Author(s):  
Shu-Chi Hsu ◽  
Ford Kirschenbaum ◽  
Judy Miller ◽  
Barbara Cordell ◽  
Justin V. McCarthy
Keyword(s):  
Human Gene ◽  
Functional Characterization ◽  
Regulatory Region ◽  
Response Factor ◽  
Upstream Regulatory Region ◽  
Gene Encoding

scholarly journals Molecular cloning and characterization of the structural gene for the major iron-regulated protein expressed by Neisseria gonorrhoeae.

1990 ◽  
Vol 171 (5) ◽  
pp. 1535-1546 ◽  
Author(s):  
S A Berish ◽  
T A Mietzner ◽  
L W Mayer ◽  
C A Genco ◽  
B P Holloway ◽  
...  
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Neisseria Gonorrhoeae ◽  
Biochemical Characterization ◽  
Iron Acquisition ◽  
Regulatory Region ◽  
Single Copy ◽  
Oligonucleotide Probes ◽  
Gene Encoding

This report describes the cloning and sequencing of the major iron-regulated protein (termed Fbp) of Neisseria gonorrhoeae strain F62. Attempts to identify recombinants expressing the Fbp using specific antibody proved unsuccessful. Therefore, an alternative cloning strategy using oligonucleotide probes derived from NH2-terminal and tryptic fragments of this protein was used to identify short fragments of the gene. Using this methodology, the gene encoding the precursor of Fbp was cloned on three separate overlapping fragments and sequenced, and the amino acid sequence was deduced. These data were unambiguously confirmed by the known NH2-terminal amino acid sequence and were supported by the sequences from tryptic fragments that lie outside of this region. Using oligonucleotide probes, we were unable to obtain clones encoding the potential regulatory region of this protein. Therefore, the technique of inverse polymerase chain reaction was used to amplify a fragment containing an additional 200 bp. This fragment was cloned and sequenced and found to contain a consensus ribosome binding site and potential -10 and -35 sequences. Hybridization analysis of genomic DNA from gonococcal strain F62 indicated that only a single copy of the Fbp gene exists per genome. These results complement the biochemical characterization of the Fbp expressed by gonococci and further suggest that it has a role in iron-acquisition.

Characterization of mutations that lie in the promoter-regulatory region for glnA, the structural gene encoding glutamine synthetase

1984 ◽  
Vol 197 (1) ◽  
pp. 150-160 ◽  
Author(s):  
Linda McCarter ◽  
Krystyna Krajewska-Grynkiewicz ◽  
Dzung Trinh ◽  
Grace Wei ◽  
Sydney Kustu
Keyword(s):  
Glutamine Synthetase ◽  
Structural Gene ◽  
Regulatory Region ◽  
Gene Encoding

Phylogeny Characterization of Seb Gene Encoding Enterotoxins in Staphylococcus aureus Isolated from Raw Milk and Cheese

2019 ◽  
Vol 9 (o3) ◽  
Author(s):  
Fatima N. Aziz ◽  
Laith Abdul Hassan Mohammed-Jawad
Keyword(s):  
Staphylococcus Aureus ◽  
Food Poisoning ◽  
Raw Milk ◽  
Staphylococcal Enterotoxin B ◽  
Human Pathogen ◽  
Conventional Pcr ◽  
Biochemical Tests ◽  
Specific Primers ◽  
Gene Encoding

Food poisoning due to the bacteria is a big global problem in economically and human's health. This problem refers to an illness which is due to infection or the toxin exists in nature and the food that use. Milk is considered a nutritious food because it contains proteins and vitamins. The aim of this study is to detect and phylogeny characterization of staphylococcal enterotoxin B gene (Seb). A total of 200 milk and cheese samples were screened. One hundred ten isolates of Staphylococcus aureus pre-confirmed using selective and differential media with biochemical tests. Genomic DNA was extracted from the isolates and the SEB gene detects using conventional PCR with specific primers. Three staphylococcus aureus isolates were found to be positive for Seb gene using PCR and confirmed by sequencing. Sequence homology showed variety range of identity starting from (100% to 38%). Phylogenetic tree analyses show that samples (6 and 5) are correlated with S. epidermidis. This study discovered that isolates (A6-RLQ and A5-RLQ) are significantly clustered in a group with non- human pathogen Staphylococcus agnetis.

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