Hydrogen peroxide-enhanced transanal ultrasound in the assessment of fistula-in-ano

1998 ◽  
Vol 41 (9) ◽  
pp. 1147-1152 ◽  
Author(s):  
A. C. Poen ◽  
R. J. F. Felt-Bersma ◽  
Q. A. J. Eijsbouts ◽  
M. A. Cuesta ◽  
S. G. M. Meuwissen
Keyword(s):  
Hydrogen Peroxide ◽  
Fistula In Ano ◽  
Transanal Ultrasound

4571 Hydrogen peroxide enhanced transanal ultrasound in fistula-in-ano. difference in track between primary and secondary perianal fistula.

2000 ◽  
Vol 51 (4) ◽  
pp. AB171 ◽  
Author(s):  
Cornelius E. Sloots ◽  
Alexander C. Poen ◽  
Richelle J. Felt ◽  
Miguel A. Cuesta ◽  
Stephan G. Meuwissen
Keyword(s):  
Hydrogen Peroxide ◽  
Perianal Fistula ◽  
Fistula In Ano ◽  
Transanal Ultrasound

Intraoperative Physical Diagnosis in the Management of Anal Fistula

2006 ◽  
Vol 72 (1) ◽  
pp. 11-15 ◽  
Author(s):  
Claudia Gonzalez-Ruiz ◽  
Andreas M. Kaiser ◽  
Petar Vukasin ◽  
Robert W. Beart ◽  
Adrian E. Ortega
Keyword(s):  
Hydrogen Peroxide ◽  
Methylene Blue ◽  
Medical Center ◽  
Physical Diagnosis ◽  
Fistula In Ano ◽  
Period Data ◽  
Hanley Procedure ◽  
Postoperative Incontinence ◽  
Prospective Database

This report reviews a prospective database applying a systematic fistulomy technique in 101 patients requiring surgery for fistula in ano at LAC+USC Medical Center during a 15-month period. Data were collected for the reliability of primary crypt palpation, success of tract injection with peroxide/methylene blue, and the accuracy of Goodsall's rule. Time to healing, recurrence, and incontinence according to type of procedure were also recorded. Palpation of the primary crypt was possible in 93 per cent. Hydrogen peroxide/methylene blue injection successfully delineated the tract in 83 per cent. Goodsall's rule was correct in 81 per cent. Each fistula was categorized as intersphincteric (n = 72), transphincteric (n = 33), extrasphincteric (n = 1), or sub-mucosal (n = 6). At a mean follow-up period of 44 weeks, 89.2 per cent of patients were cured. Reasons for recurrence included wound bridging (n = 6), misdiagnosis of the tract (n = 3), and two blind-ended fistulae (n = 2). Time to healing in weeks was (mean, range): simple fistulotomy (12, 3–21), seton (16, 4–28), Hanley procedure (28, 8–48). Patients with a marsupialized tract healed at an average of 6 weeks (range 4–8). Four (3.9%) patients reported postoperative incontinence (1 gas, 3 liquid, 0 solids).

Value of Hydrogen Peroxide Enhancement of Three-Dimensional Endoanal Ultrasound in Fistula-in-Ano

2005 ◽  
Vol 48 (1) ◽  
pp. 141-147 ◽  
Author(s):  
Gordon N. Buchanan ◽  
Clive I. Bartram ◽  
Andrew B. Williams ◽  
Steve Halligan ◽  
Richard C. G. Cohen
Keyword(s):  
Hydrogen Peroxide ◽  
Three Dimensional ◽  
Endoanal Ultrasound ◽  
Fistula In Ano

Peroxidase Localization in Hartmanella Trophozoites

1971 ◽  
Vol 29 ◽  
pp. 346-347 ◽  
Author(s):  
George E. Childs ◽  
Joseph H. Miller
Keyword(s):  
Hydrogen Peroxide ◽  
Ultrastructural Localization ◽  
Pathogenic Strain ◽  
Oxidative Enzymes ◽  
Differential Centrifugation ◽  
Electron Microscopic ◽  
Microscopic Studies ◽  
The Subject ◽  
Axenic Cultures

Biochemical and differential centrifugation studies have demonstrated that the oxidative enzymes of Acanthamoeba sp. are localized in mitochondria and peroxisomes (microbodies). Although hartmanellid amoebae have been the subject of several electron microscopic studies, peroxisomes have not been described from these organisms or other protozoa. Cytochemical tests employing diaminobenzidine-tetra HCl (DAB) and hydrogen peroxide were used for the ultrastructural localization of peroxidases of trophozoites of Hartmanella sp. (A-l, Culbertson), a pathogenic strain grown in axenic cultures of trypticase soy broth.

Fluorescent proteins for in vivo imaging, where's the biliverdin?

2020 ◽  
Vol 48 (6) ◽  
pp. 2657-2667
Author(s):  
Felipe Montecinos-Franjola ◽  
John Y. Lin ◽  
Erik A. Rodriguez
Keyword(s):  
Hydrogen Peroxide ◽  
In Vivo Imaging ◽  
Near Infrared ◽  
Fluorescent Protein ◽  
Fluorescent Proteins ◽  
Fluorescent Imaging ◽  
Infrared Light ◽  
Red Fluorescent Protein ◽  
Color Palette

Noninvasive fluorescent imaging requires far-red and near-infrared fluorescent proteins for deeper imaging. Near-infrared light penetrates biological tissue with blood vessels due to low absorbance, scattering, and reflection of light and has a greater signal-to-noise due to less autofluorescence. Far-red and near-infrared fluorescent proteins absorb light >600 nm to expand the color palette for imaging multiple biosensors and noninvasive in vivo imaging. The ideal fluorescent proteins are bright, photobleach minimally, express well in the desired cells, do not oligomerize, and generate or incorporate exogenous fluorophores efficiently. Coral-derived red fluorescent proteins require oxygen for fluorophore formation and release two hydrogen peroxide molecules. New fluorescent proteins based on phytochrome and phycobiliproteins use biliverdin IXα as fluorophores, do not require oxygen for maturation to image anaerobic organisms and tumor core, and do not generate hydrogen peroxide. The small Ultra-Red Fluorescent Protein (smURFP) was evolved from a cyanobacterial phycobiliprotein to covalently attach biliverdin as an exogenous fluorophore. The small Ultra-Red Fluorescent Protein is biophysically as bright as the enhanced green fluorescent protein, is exceptionally photostable, used for biosensor development, and visible in living mice. Novel applications of smURFP include in vitro protein diagnostics with attomolar (10−18 M) sensitivity, encapsulation in viral particles, and fluorescent protein nanoparticles. However, the availability of biliverdin limits the fluorescence of biliverdin-attaching fluorescent proteins; hence, extra biliverdin is needed to enhance brightness. New methods for improved biliverdin bioavailability are necessary to develop improved bright far-red and near-infrared fluorescent proteins for noninvasive imaging in vivo.

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