Intestinal fat and iron transport, goblet cell mucus secretion, and cellular changes in protein deficiency observed with the electron microscope

1965 ◽  
Vol 10 (12) ◽  
pp. 1004-1023 ◽  
Author(s):  
James A. Freeman ◽  
Jack C. Geer
Keyword(s):  
Electron Microscope ◽  
Goblet Cell ◽  
Iron Transport ◽  
Protein Deficiency ◽  
Mucus Secretion ◽  
Cellular Changes ◽  
Cell Mucus

scholarly journals New developments in goblet cell mucus secretion and function

2015 ◽  
Vol 8 (4) ◽  
pp. 712-719 ◽  
Author(s):  
G M H Birchenough ◽  
M EV Johansson ◽  
J K Gustafsson ◽  
J H Bergström ◽  
G C Hansson
Keyword(s):  
Goblet Cell ◽  
Mucus Secretion ◽  
New Developments ◽  
And Function ◽  
Cell Mucus

scholarly journals NLRP6 Inflammasome Orchestrates the Colonic Host-Microbial Interface by Regulating Goblet Cell Mucus Secretion

Cell ◽  
2014 ◽  
Vol 156 (5) ◽  
pp. 1045-1059 ◽  
Author(s):  
Marta Wlodarska ◽  
Christoph A. Thaiss ◽  
Roni Nowarski ◽  
Jorge Henao-Mejia ◽  
Jian-Ping Zhang ◽  
...  
Keyword(s):  
Goblet Cell ◽  
Mucus Secretion ◽  
Cell Mucus

scholarly journals Novel Human NKCC1 Mutations Cause Defects in Goblet Cell Mucus Secretion and Chronic Inflammation

2020 ◽  
Vol 9 (2) ◽  
pp. 239-255 ◽  
Author(s):  
Rainelli Koumangoye ◽  
Salma Omer ◽  
Mustafa H. Kabeer ◽  
Eric Delpire
Keyword(s):  
Chronic Inflammation ◽  
Goblet Cell ◽  
Mucus Secretion ◽  
Cell Mucus

scholarly journals Malignant H1299 tumour cells preferentially internalize iron-bound inositol hexakisphosphate

2013 ◽  
Vol 33 (5) ◽  
Author(s):  
Christina Helmis ◽  
Christine Blechner ◽  
Hongying Lin ◽  
Michaela Schweizer ◽  
Georg W. Mayr ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Electron Microscope ◽  
Iron Uptake ◽  
Iron Transport ◽  
Bronchial Carcinoma ◽  
Bound State ◽  
Tumour Cells ◽  
Inositol Hexakisphosphate ◽  
Metal Free ◽  
Well Differentiated

In colon enterocytes and in well-differentiated colon cancer CaCo-2 cells, InsP6 (inositol hexakisphosphate) inhibits iron uptake by forming extracellular insoluble iron/InsP6 complexes. In this study, we confirmed that CaCo-2 cells are not able to take up iron/InsP6 but, interestingly, found that the cells are able to internalize metal-free and Cr3+-bound InsP6. Thus, the inability of CaCo-2 cells to take up iron/InsP6 complexes seems to be due to the iron-bound state of InsP6. Since recently we demonstrated that the highly malignant bronchial carcinoma H1299 cells internalize and process InsP6, we examined whether these cells may be able to take up iron/InsP6 complexes. Indeed, we found that InsP6 dose-dependently increased uptake of iron and demonstrated that in the iron-bound state InsP6 is more effectively internalized than in the metal-free or Cr3+-bound state, indicating that H1299 cells preferentially take up iron/InsP6 complexes. Electron microscope and cell fraction assays indicate that after uptake H1299 cells mainly stored InsP6/iron in lysosomes as large aggregates, of which about 10% have been released to the cytosol. However, this InsP6-mediated iron transport had no significant effects on cell viability. This result together with our finding that the well-differentiated CaCo-2 cells did not, but the malignant H1299 cells preferentially took up iron/InsP6, may offer the possibility to selectively transport cytotoxic substances into tumour cells.

scholarly journals SYNTHESIS OF THE CARBOHYDRATE OF MUCUS IN THE GOLGI COMPLEX AS SHOWN BY ELECTRON MICROSCOPE RADIOAUTOGRAPHY OF GOBLET CELLS FROM RATS INJECTED WITH GLUCOSE-H3

1966 ◽  
Vol 30 (1) ◽  
pp. 119-136 ◽  
Author(s):  
Marian Neutra ◽  
C. P. Leblond
Keyword(s):  
Electron Microscope ◽  
Goblet Cell ◽  
Golgi Complex ◽  
Apical Membrane ◽  
Goblet Cells ◽  
Complex Carbohydrate ◽  
Golgi Stack ◽  
Mucus Glycoprotein ◽  
Almost All

It is known that colonic goblet cells utilize glucose to synthesize the carbohydrate portion of mucus glycoprotein. To determine the intracellular site of this synthesis, glucose-H3 was injected into 10-g rats. At 5, 20, 40 min, 1, 1½, and 4 hr after injection, segments of colon were fixed and prepared for electron microscope radioautography. By 5 min after injection, label had been incorporated into substances present in the flattened saccules of the Golgi complex. At 20 min, both Golgi saccules and nearby mucigen granules were labeled. By 40 min, mucigen granules carried almost all detectable radioactivity. Between 1 and 4 hr, these labeled granules migrated from the supranuclear region to the apical membrane; here, they were extruded singly, retaining their limiting membrane. The evidence indicates that the Golgi saccule is the site where complex carbohydrate is synthesized and is added to immigrant protein to form the complete glycoprotein of mucus. The Golgi saccule, distended by this material, becomes mucigen granules. It is roughly estimated that one saccule is released by each Golgi stack every 2 to 4 min: a conclusion implying continuous renewal of Golgi stacks. It appears that the Golgi synthesis, intracellular migration, and release of mucus glycoprotein occur continually throughout the life of the goblet cell.

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