Absence of mitochondrial terminal respiratory enzymes in cartilage matrix vesicles

1977 ◽  
Vol 24 (1) ◽  
pp. 37-39 ◽  
Author(s):  
P. Person ◽  
S. Papierman ◽  
H. Zipper ◽  
R. M. Libbin
Keyword(s):  
Matrix Vesicles ◽  
Cartilage Matrix ◽  
Respiratory Enzymes

Effects of EDTA, Hyaluronidase and Collagenase on Epiphyseal Cartilage Matrix

1968 ◽  
Vol 26 ◽  
pp. 56-57
Author(s):  
H. Clarke Anderson ◽  
Priscilla R. Coulter
Keyword(s):  
Light And Electron Microscopy ◽  
Matrix Vesicles ◽  
Cartilage Matrix ◽  
Collagen Fibrils ◽  
Epiphyseal Cartilage ◽  
Dense Matrix ◽  
Type Iv ◽  
Bovine Testicular Hyaluronidase ◽  
The Matrix ◽  
Testicular Hyaluronidase

Epiphyseal cartilage matrix contains fibrils and particles of at least 5 different types: 1. Banded collagen fibrils, present throughout the matrix, but not seen in the lacunae. 2. Non-periodic fine fibrils <100Å in diameter (Fig. 1), which are most notable in the lacunae, and may represent immature collagen. 3. Electron dense matrix granules (Fig. 1) which are often attached to fine fibrils and collagen fibrils, and probably contain protein-polysaccharide although the possibility of a mineral content has not been excluded. 4. Matrix vesicles (Fig. 2) which show a selective distribution throughout the epiphysis, and may play a role in calcification. 5. Needle-like apatite crystals (Fig. 2).Blocks of formalin-fixed epiphysis from weanling mice were digested with the following agents in 0.1M phosphate buffer: a) 5% ethylenediaminetetraacetate (EDTA) at pH 8.3, b) 0.015% bovine testicular hyaluronidase (Sigma, type IV, 750 units/mg) at pH 5.5, and c) 0.1% collagenase (Worthington, chromatograhically pure, 200 units/mg) at pH 7.4. All digestions were carried out at 37°C overnight. Following digestion tissues were examined by light and electron microscopy to determine changes in the various fibrils and particles of the matrix.

scholarly journals From Matrix Vesicles to Miniature Rocks: Evolution of Calcium Deposits in Calf Costochondral Junctions

Cartilage ◽  
2020 ◽  
pp. 194760352094122
Author(s):  
Jakub Jaroszewicz ◽  
Piotr Bazarnik ◽  
Anna Osiecka-Iwan ◽  
Anna Hyc ◽  
Emilia Choinska ◽  
...  
Keyword(s):  
Matrix Vesicles ◽  
Cartilage Matrix ◽  
X Ray Diffraction ◽  
3 Dimensional ◽  
Force Microscopy ◽  
Proliferative Zone ◽  
Atomic Force ◽  
Calcified Cartilage ◽  
Zone Of Provisional Calcification ◽  
Calcium Deposits

Objective Initial stages of cartilage matrix calcification depend on the activity of matrix vesicles. The purpose of the study was to describe how calcified matrix vesicles join into larger structures, to present their up-to-date undescribed 3-dimensional image, and to observe how calcified matrix relates to chondrocyte lacunae. Design Calcified cartilage was obtained from the zone of provisional calcification of calf costochondral junctions, then enzymatically isolated and studied by microtomography, scanning electron microscopy, atomic force microscopy and X-ray diffraction, and Fourier transform infrared spectroscopy. Results Hyaluronidase digestion released packets of granules surrounded by the cartilage matrix. Further digestion, with collagenase and trypsin, removed matrix and exposed granules with dimensions within 50 to 150 nm range, which we consider as equivalent of calcified matrix vesicles. Granules joined into larger groups with dimensions of 0.5 to 2 μm, which we call globular units. Certain matrix vesicles appeared well connected but contained globular units that had spaces filled with electron lucent material, presumably matrix or chondrocyte remnants. Globular units were organized into massive structures taking the shape of oval plates. Comparison of these plates with lacunae containing isogenous groups of chondrocytes from proliferative zone of costochondral junction suggests that the cells from a single lacuna were responsible for the formation of one plate. The plates were connected with each other and extended over provisional calcification zone. Conclusions The outcome showed how particular calcified matrix vesicles associate into globular units, which organize into massive structures assuming the shape of oval plates and eventually cover large areas of cartilage matrix.

Pyrophosphatase and ATPase of isolated cartilage matrix vesicles

1977 ◽  
Vol 22 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Rolf Felix ◽  
Herbert Fleisch
Keyword(s):  
Matrix Vesicles ◽  
Cartilage Matrix

scholarly journals 175 ATP-DEPENDENT MINERALIZATION OF HYALINE ARTICULAR CARTILAGE MATRIX VESICLES

1988 ◽  
Vol 24 (1) ◽  
pp. 140-140
Author(s):  
John W Rachow ◽  
Majeedul H Chowdhurry ◽  
Robert L Wortmann
Keyword(s):  
Articular Cartilage ◽  
Matrix Vesicles ◽  
Cartilage Matrix ◽  
Hyaline Articular Cartilage
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