Cloning and characterization of aSchizophyllum gene withAβ6 mating-type activity

1996 ◽  
Vol 29 (2) ◽  
pp. 136-142 ◽  
Author(s):  
Guang-Ping Shen ◽  
Dong-Chul Park ◽  
Robert C. Ullrich ◽  
Charles P. Novotny
Keyword(s):  
Mating Type ◽  
Type Activity

Cloning and characterization of a Schizophyllum gene with Aβ6 mating-type activity

1996 ◽  
Vol 29 (2) ◽  
pp. 136-142 ◽  
Author(s):  
G.-P. Shen ◽  
Dong-Chul Park ◽  
Robert C. Ullrich ◽  
Charles P. Novotny
Keyword(s):  
Mating Type ◽  
Type Activity

scholarly journals Mating Type in Chlamydomonas Is Specified by mid, the Minus-Dominance Gene

Genetics ◽  
1997 ◽  
Vol 146 (3) ◽  
pp. 859-869 ◽  
Author(s):  
Patrick J Ferris ◽  
Ursula W Goodenough
Keyword(s):  
Transcription Factor ◽  
Mating Type ◽  
Codon Bias ◽  
Leucine Zipper ◽  
Laboratory Strain ◽  
Leucine Zipper Motif ◽  
Type Locus ◽  
Diploid Cells ◽  
Necessary And Sufficient

Diploid cells of Chlamydomonas reinhardtii that are heterozygous at the mating-type locus (mt  +/mt  –) differentiate as minus gametes, a phenomenon known as minus dominance. We report the cloning and characterization of a gene that is necessary and sufficient to exert this minus dominance over the plus differentiation program. The gene, called mid, is located in the rearranged (R) domain of the mt  – locus, and has duplicated and transposed to an autosome in a laboratory strain. The imp11 mt  – mutant, which differentiates as a fusion-incompetent plus gamete, carries a point mutation in mid. Like the fus1 gene in the mt  + locus, mid displays low codon bias compared with other nuclear genes. The mid sequence carries a putative leucine zipper motif, suggesting that it functions as a transcription factor to switch on the minus program and switch off the plus program of gametic differentiation. This is the first sex-determination gene to be characterized in a green organism.

scholarly journals Cloning and characterization of four SIR genes of Saccharomyces cerevisiae.

1986 ◽  
Vol 6 (2) ◽  
pp. 688-702 ◽  
Author(s):  
J M Ivy ◽  
A J Klar ◽  
J B Hicks
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Mating Type ◽  
Blot Analysis ◽  
Transcriptional Control ◽  
Genomic Library ◽  
Yeast Saccharomyces Cerevisiae ◽  
Mating Type Genes ◽  
Rna Blots

Mating type in the yeast Saccharomyces cerevisiae is determined by the MAT (a or alpha) locus. HML and HMR, which usually contain copies of alpha and a mating type information, respectively, serve as donors in mating type interconversion and are under negative transcriptional control. Four trans-acting SIR (silent information regulator) loci are required for repression of transcription. A defect in any SIR gene results in expression of both HML and HMR. The four SIR genes were isolated from a genomic library by complementation of sir mutations in vivo. DNA blot analysis suggests that the four SIR genes share no sequence homology. RNA blots indicate that SIR2, SIR3, and SIR4 each encode one transcript and that SIR1 encodes two transcripts. Null mutations, made by replacement of the normal genomic allele with deletion-insertion mutations created in the cloned SIR genes, have a Sir- phenotype and are viable. Using the cloned genes, we showed that SIR3 at a high copy number is able to suppress mutations of SIR4. RNA blot analysis suggests that this suppression is not due to transcriptional regulation of SIR3 by SIR4; nor does any SIR4 gene transcriptionally regulate another SIR gene. Interestingly, a truncated SIR4 gene disrupts regulation of the silent mating type loci. We propose that interaction of at least the SIR3 and SIR4 gene products is involved in regulation of the silent mating type genes.

scholarly journals Distribution and mating type characterization of chickpea blight (Didymella rabiei (Kov.) v. Arx) in Ethiopia

2021 ◽  
pp. 100220
Author(s):  
Gezahegne Getaneh ◽  
Tadele Tefera ◽  
Fikre Lemmessa ◽  
Seid Ahmed ◽  
Jandouwe Villinger
Keyword(s):  
Mating Type

scholarly journals Characterization of mating-type loci in rice false smut fungus Villosiclava virens

2015 ◽  
Vol 362 (9) ◽  
Author(s):  
Jun-jie Yu ◽  
Wen-xian Sun ◽  
Mi-na Yu ◽  
Xiao-le Yin ◽  
Xiang-kun Meng ◽  
...  
Keyword(s):  
Mating Type ◽  
Smut Fungus ◽  
Rice False Smut ◽  
Villosiclava Virens ◽  
False Smut

scholarly journals Characterization of Natural Isolates of Bipolaris maydis Associated with Mating Types, Genetic Diversity, and Pathogenicity in Fujian Province, China

Plant Disease ◽  
2020 ◽  
Vol 104 (2) ◽  
pp. 323-329
Author(s):  
Yuli Dai ◽  
Lin Gan ◽  
Hongchun Ruan ◽  
Niuniu Shi ◽  
Yixin Du ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Mating Type ◽  
Dna Polymorphism ◽  
Sweet Corn ◽  
Issr Markers ◽  
Bipolaris Maydis ◽  
Fujian Province ◽  
Field Isolates ◽  
Rational Distribution

Due to the natural destructiveness and persistence of the southern corn leaf blight (SCLB) fungus Bipolaris maydis (Nisikado et Miyake) Shoem, the characterization of B. maydis field isolates is essential to guide the rational distribution of resistant materials in corn-growing regions. In the present study, 102 field isolates collected from seven locations covering the entire region of Fujian Province, China, were assessed for mating type distribution, genetic diversity, and pathogenicity toward local sweet corn cultivars. Mating type detection via polymerase chain reaction indicated that 36.3 and 63.7% of isolates were MAT1-1 and MAT1-2, respectively; more than 80% of these isolates were confirmed using cross assays with known mating type isolates. Thirteen intersimple sequence repeat (ISSR) markers within and among two mating type populations revealed a high level of DNA polymorphism for all combined isolates and between MAT1-1 and MAT1-2 populations. The MAT1-2 population was more diverse based on DNA polymorphism than the MAT1-1 population. The value of GST was 0.0070, ranging from 0.0399 to 0.3044 based on analysis of combined isolates and individual regional populations, respectively, suggesting the presence of genetic differentiation in the two mating type populations from different locations. Pathogenicity assays revealed that both MAT1-1 and MAT1-2 populations were pathogenic to all 11 local sweet corn cultivars tested in this study. The potential of sexual reproduction, existence of genetic diversity in the two mating type populations, and pathogenicity suggest that B. maydis populations have independently clonally adapted under natural field conditions during corn cultivation.

scholarly journals Characterization of mating type genes in heterothallic Neonectria species, with emphasis on N. coccinea, N. ditissima, and N. faginata

Mycologia ◽  
2020 ◽  
Vol 112 (5) ◽  
pp. 880-894 ◽  
Author(s):  
Cameron M. Stauder ◽  
Jeff R. Garnas ◽  
Eric W. Morrison ◽  
Catalina Salgado-Salazar ◽  
Matt T. Kasson
Keyword(s):  
Mating Type ◽  
Mating Type Genes

scholarly journals Release of a Potent Polymorphonuclear Leukocyte Chemoattractant Is Regulated by White-Opaque Switching in Candida albicans

2004 ◽  
Vol 72 (2) ◽  
pp. 667-677 ◽  
Author(s):  
Jeremy Geiger ◽  
Deborah Wessels ◽  
Shawn R. Lockhart ◽  
David R. Soll
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Candida Albicans ◽  
Mating Type ◽  
Related Species ◽  
Polymorphonuclear Leukocytes ◽  
White Phase ◽  
Bona Fide ◽  
Human Polymorphonuclear Leukocytes ◽  
Cell Chemotaxis

ABSTRACT Previous studies employing transmembrane assays suggested that Candida albicans and related species, as well as Saccharomyces cerevisiae, release chemoattractants for human polymorphonuclear leukocytes (PMNs). Because transmembrane assays do not definitively distinguish between chemokinesis and chemotaxis, single-cell chemotaxis assays were used to confirm these findings and test whether mating-type or white-opaque switching affects the release of attractant. Our results demonstrate that C. albicans, C. dubliniensis, C. tropicalis, C. parapsilosis, and C. glabrata release bona fide chemoattractants for PMNs. S. cerevisiae, however, releases a chemokinetic factor but not a chemoattractant. Characterization of the C. albicans chemoattractant revealed that it is a peptide of approximately 1 kDa. Whereas the mating type of C. albicans did not affect the release of chemoattractant, switching did. White-phase cells released chemoattractant, but opaque-phase cells did not. Since the opaque phase of C. albicans represents the mating-competent phenotype, it may be that opaque-phase cells selectively suppress the release of chemoattractant to facilitate mating.

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